FIGURE 5: t6A deficient strains accumulate aggregated proteins and Advanced Glycated End-products (AGEs).

(A) Protein aggregation in BY4742 and mutant yeast cells. Yeast were grown in YPD to an OD600 = 0.8. Soluble and aggregated proteins were separated by SDS–PAGE and visualized by Coomassie blue staining.

(B) AGEs were visualized by silver staining.

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