FIGURE 5: Acidification-independent impact of spent medium composition on lifespan.

Cells were grown on 0.1% and 0.4% glycerol (A) as well as 0.2% and 0.6% glucose (B) for 20 hours. The spent medium from cultures grown on one concentration of carbon source was replaced by the spent medium originating from cultures containing other concentration of that carbon source with pH adjusted to 6.5. Data represent mean viability ± SD from 3 independent cultures. Representative data of two experiments with similar results are presented.