FIGURE 10: Ultrastructural analysis of IGP48 RNAi cells reveals defects in the ER. Transmission electron micrographs of IGP48 RNAi cell lines.

Top left: Representative uninduced IGP48 RNAi cell with major secretory pathway organelles indicated. Other panels are induced cells after 24 hours induction.

Top centre: Distorted ER with apparent lumenal inclusion.

Top right: ER tubules with apparent normal morphology.

Lower left: Extensive vesicles associated with the Golgi complex. Based on observations that the Golgi complex is concave towards the trans-face (see top left panel), these vesicles are likely ER to Golgi transport intermediates corresponding to a structure similar to the ERGIC, i.e. ER-GIC-like.

Lower right: Examples of extensive clusters of vesicles in close association with ER tubules. Scale bars are 500 nm.

Abbreviations: ER, endoplasmic reticulum; ERGIC, ER-Golgi intermediate compartment; TGN, trans-Golgi network.

For figures in higher resolution please refer to PDF version of the article.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.