FIGURE 6: Real-time PCR analysis of mRNA levels in yeast cells.

(A) Analysis of PAP mRNA in yeast cells expressing AtBI-1.

(B) Analysis of AtBI-1 mRNA in yeast cells expressing PAP. Cells were grown on galactose for the hours indicated.

The mRNA levels for the genes were normalized to G6PD mRNA using the ΔΔCT method from Applied Biosystems. The results represent three independent experiments. Columns are statistically different according to ANOVA (P < 0.001) followed by a post-hoc Fisher’s Least Significant Difference (LSD) test.

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