FIGURE 6: Reciprocal hemizygosity analysis (RHA) and allele exchange of the PMA1 and TPS1 al-leles. Ethyl acetate production in semi-anaerobic fermentations after 96 h. (A) RHA of PMA1 in the TDA1(4)/S288c hybrid strain. (B) Allele replacement of PMA1S288c by PMA1TDA1(4) in the S288c strain. (C) RHA of TPS1 in the ER7A/S288c hybrid strain. (D) Allele replacement of TPS1ER7A by TPS1S288c in the ER7A strain. The results were analysed with an unpaired, two-sided Student t-test with p-value ≤0.001 (***).

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