FIGURE 7: Reciprocal hemizygosity analysis (RHA) and allele exchange of the CEM1 allele. Ethyl acetate production in semi-anaerobic fermentations after 96 h. (A) RHA of CEM1 in the TDA1(4)/S288c hybrid strain. (B) Allele replacement of CEM1^S288c by CEM1^TDA1(4) in the S288c strain. (C) Allele replacement of both CEM1^S288c and PMA1^S288c by CEM1^TDA1(4) and PMA1^TDA1(4), respectively, in the S288c strain. (D) Allele replacement of CEM1^TDA1(4) by CEM1^S288c in the TDA1(4) strain. (E) Allele replacement of CEM1^TDA1(4) by CEM1^S288c in the five segregants of TDA1(4)/S288c with the lowest ethyl acetate production. The results were analysed with an unpaired, two-sided Student t-test with p-value ≤0.05 (*) or ≤0.01 (**).