FIGURE 8: Ste11 is functionally redundant with Bck1 following 1.2 mM H2O2 stress.

(A) Myc-cyclin C levels were monitored as described previously in ste11∆ (RSY1983) and ste11 bck1∆ strain (RSY1985) exposed to 1.2 mM H2O2 for the times indicated. Tub1 levels were used as a loading control.

(B) Quantification of the results obtained in (A).

(C) Ste11 is required for normal cyclin C nuclear release following 1.2 mM stress. Fluorescence microscopy was used to monitor cyclin C-YFP localization in ste11∆ (BLY21) and bck1Δ ste11∆ (BLY478) cells before (0 h) and following (2 h) 1.2 mM H2O2 treatment. Representative images (collapsed de-convolved 0.2 µm slices) of the results obtained are shown. Perinuclear and cytoplasmic localization of cyclin C-YFP are indicated by the red and yellow arrows, respectively.

(D) Quantification of the results in (C). The percent of the population displaying at least 3 cyclin C-YFP foci in the cytoplasm is given (mean ± s.e.m.). At least 200 cells were counted per time point from 3 individual isolates.

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