Back to article: Transcriptomic and chemogenomic analyses unveil the essential role of Com2-regulon in response and tolerance of Saccharomyces cerevisiae to stress induced by sulfur dioxide

FIGURE 1. Comparison of the susceptibility to SO2 of the Saccharomyces cerevisiae BY4741 parental strain and the deletion mutants Δmsn2, Δmsn4, Δhaa1 and Δcom2 by spot assays (A) or by cultivation in MMB liquid medium (pH 3.5) (▪) or in this medium supplemented with 0.5 mM of SO2 (□) (B). In (A) the cells used to prepare the spots were grown in MMB liquid medium until mid-exponential phase and then inoculated (at an OD of 0.05) in MMB (pH 3.5) agarized medium supplemented with the indicated concentrations of SO2. Lanes (b) and (c) are, respectively, 1:5 and 1:10 dilutions of the suspension used in lane (a). In (B) growth was followed by measuring culture OD600 and the concentration of viable cells was assessed as the number of colony forming units per ml of cell culture (CFU ml-1). All the results presented are representative of at least three independent experiments that gave the same pattern of results.

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