FIGURE 3: Up-regulation of Osh6 delays the secretion of Pma1. (A) Comparison of the localization of Pma1-mCherry in wild type (QAY559) and P_ERG6–OSH6 (FTY518). Cells were grown to early log phase and photographed. The arrow points to vacuole-like structures of intracellular Pma1. The arrowhead points to a bud without normal Pma1 on the PM. (B). Zygote assay. A MATα strain of Pma1-GFP in wild type (HHY103) was mated with a MATa strain of Pma1-mCherry in P_ERG6–OSH6 (FTY517) and the resulting zygotes were photographed. Zygotes with different sizes of buds were presented from the top row (smallest bud) to the bottom row (largest bud). Arrows point to buds.