FIGURE 5: Up-regulated Osh6 works through the TGN-LE trafficking pathway. (A) The Pma1-mCherry distribution in the indicated strains. The respective control for each indicated mutant is shown on the left with Pma1-mCherry. P_ERG6–OSH6 side means P_ERG6–OSH6 Pma1-mCherry carrying the indicated mutations listed on the left. Early log phase cells grown in YEPD were photographed. (B) Comparison of growth on metal-containing media. Five μl of 10-fold serially diluted cells (starting from the left 0.1 OD₆₀₀/ml) were spotted on YEPD or YEPD with the indicated salts and grown at 30°C for two days.