FIGURE 6. Effect of acetate on the capability of L. gasseri to induce loss of C. glabrata cellular viability in mixed biofilms. Biofilms formed by L. gasseri and C. glabrata after 24 h of single or co-cultivation in MRS medium with increasing amounts of acetate at 37ºC and 25 rpm were imaged by fluorescence confocal microscopy to distinguish between live and dead cells, as detailed in materials and methods. The pictures presented are representative of a cohort obtained in three independent replicas and that were used to quantify the number of dead C. glabrata cells (labelled in red) compared to the total number of C. glabrata cells in the field (labelled in green and distinguishable from the bacterial cells based on their yeast-like morphology). All scale bars corresponds to 100 μm. The result of the quantification is shown in the chart presented at the right. The data presented concerning the number of C. glabrata cells in mixed biofilms formed in MRS having 60 mM acetate is the one shown in Fig.3. Statistical significance of the differences obtained in the number of dead yeast cells in the different conditions was calculated using one-way ANOVA (*p-value below 0.05; **p-value below 0.01; ***p-value below 0.001; ****p-value below 0.0001).