FIGURE 2: Expression of sfGFP^m does not disturb mitochondrial function. (A) Wild type (WT) and sfGFP^m were streaked out on fermentable (Glucose) and non-fermentable (Glycerol) medium. (B) Doubling time during exponential growth phase in glucose (Glc) and glycerol (Gly). Data represent the mean of three independent experiments +/- SD. (C) Steady state levels of OXPHOS subunits in WT and sfGFP^m during exponential phase in glucose (Glc), galactose (Gal) or glycerol (Gly). Whole cell extracts were separated on SDS-PAGE and analyzed with Western Blotting using the indicated antibodies. (D) Isolated mitochondria of WT and sfGFP^m were lysed in digitonin and protein complexes were separated by blue-native PAGE. Supercomplexes (III2IV and III₂IV₂) were either visualized by Coomassie staining or complex IV in-gel activity assay.