Back to article: Decreasing cytosolic translation is beneficial to yeast and human Tafazzin-deficient cells

FIGURE 2: Genetic ablation of REI1 or RPL6B in taz1Δ yeast does not restore cardiolipin remodeling. Lipids were extracted from mitochondria isolated from WT (black bars), taz1Δ (open bars), taz1Δ rei1Δ (grey bars) and taz1Δ rpl6bΔ (striped bars) cells grown in CSM 0.5% galactose + 2% ethanol at 36°C until a density of 2-3 OD600nm. (A) Relative contents of PE (phosphatidylethanolamine), CL (cardiolipin), PI (phosphatidylinositol) and PC (phosphatidylcholine) within each strain. (B) Relative fatty acid chain composition of CL within each strain (16:0, palmitic acid; 16:1, palmitoleic acid; 18:0, stearic acid; 18:1: oleic acid). Statistical analysis was done with Kruskal-Wallis test followed by Dunn’s multiple comparison test (*P<0.05; **P<0.01; ***P<0.001). Data are expressed as mean ± s.d. (n=4). The data for WT and taz1Δ strains were reported previously [60].

60. de Taffin de Tilques M, Tribouillard-Tanvier D, Tetaud E, Testet E, di Rago JP, Lasserre JP (2017). Overexpression of mitochondrial oxodicarboxylate carrier (ODC1) preserves oxidative phosphorylation in a yeast model of Barth syndrome. Dis Model Mech 10(4): 439-450.

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