Back to article: Single telomere length analysis in Ustilago maydis, a high-resolution tool for examining fungal telomere length distribution and C-strand 5’-end processing
FIGURE 6: Characterization of the telomere C-strand 5’ end nucleotide in blm∆ and trt1∆. (A) (Top) DNA from blm∆ was ligated separately to each of six individual telorette oligo, amplified using the UT4/5-F and teltail primers, and then subjected to Southern analysis. (Bottom) DNA from trt1∆ was ligated separately to each of six individual telorette oligo, amplified using the UT4/5-F and teltail primers, and then subjected to Southern analysis. (B) The number of STELA products generated by each telorette oligo was determined for the blm∆ and trt1∆ DNA, and plotted as the percentage of total STELA products. (C) The predominant 5’ end nucleotides for the various U. maydis strains are indicated.