Research, Research Articles

Type II-Metacaspases are involved in cell stress but not in cell death in the unicellular green alga Dunaliella tertiolecta

M. Teresa Mata1,&, Armando Palma1, Candela García-Gómez1,#, María López-Parages1, Víctor Vázquez1, Iván Cheng-Sánchez2, Francisco Sarabia2, Félix López-Figueroa1, Carlos Jiménez1 and María Segovia1

This article shows that in the green alga Dunaliella tertiolecta, Type-II metacaspases are involved in the stress response to ultraviolet radiation but are not linked to cell death, suggesting their role in survival strategies under stressful environmental conditions.

Transcriptomic and chemogenomic analyses unveil the essential role of Com2-regulon in response and tolerance of Saccharomyces cerevisiae to stress induced by sulfur dioxide

Patrícia Lage1,2, Belém Sampaio-Marques3,4, Paula Ludovico3,4, Nuno P. Mira5 and Ana Mendes-Ferreira1,2

This article shows that in the presence of sulfur dioxide (SO2), the transcription factor Com2 plays a critical role in the tolerance and response of Saccharomyces cerevisiae, affecting the expression of a majority of SO2-activated genes and contributing to the protection against stress induced by SO2 at an enologically relevant pH.

Proline metabolism regulates replicative lifespan in the yeast Saccharomyces cerevisiae

Yukio Mukai1, Yuka Kamei1, Xu Liu1, Shan Jiang1, Yukiko Sugimoto2, Noreen Suliani binti Mat Nanyan2, Daisuke Watanabe2 and Hiroshi Takagi2

This article shows that intracellular proline levels in the budding yeast Saccharomyces cerevisiae are correlated with its replicative lifespan, suggesting a protective role of proline against cellular senescence due to various stresses.

Network dynamics of the yeast methyltransferome

Guri Giaever1, Elena Lissina1 and Corey Nislow1

This article presents a systematic genetic analysis of methyltransferases (MTases) under normal and stress conditions, uncovering the complex and adaptive nature of the methyltransferome and discovering a potential connection between phospholipid methylation and histone methylation, suggesting interplay between lipid homeostasis and epigenetic regulation.

Functional link between mitochondria and Rnr3, the minor catalytic subunit of yeast ribonucleotide reductase

Isaac Corcoles-Saez1, Jean-Luc Ferat2, Michael Costanzo3, Charles M. Boone3 and Rita S. Cha1

This article shows that the carbon source affects the abundance of ribonucleotide reductase (RNR) subunits in yeast, with a novel Mec1 signaling axis regulating Rnr3 independently of known DNA damage response pathways, and reveals Rnr3's unexpected role in mitochondrial function.

Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release

Alexandre Légiot1, Claire Céré1, Thibaud Dupoiron1, Mohamed Kaabouni1, Nadine Camougrand1 and Stéphen Manon1

This study investigated the role of Mitochondria-Associated Membranes (MAMs) in the regulation of apoptosis by analyzing the localization and function of the pro-apoptotic protein Bax in yeast, finding that disruption of MAMs by deletion of the MDM34 gene affects Bax's mitochondrial localization and the release of cytochrome c.

Chlamydia pneumoniae is present in the dental plaque of periodontitis patients and stimulates an inflammatory response in gingival epithelial cells

Cássio Luiz Coutinho Almeida-da-Silva1, Tamer Alpagot2, Ye Zhu1, Sonho Sierra Lee3,4, Brian P. Roberts5, Shu-Chen Hung1, Norina Tang1,2 and David M. Ojcius1

This study found that Chlamydia pneumoniae is present more frequently in the dental plaque of individuals with periodontal disease, can invade human gingival epithelial cells causing inflammatory responses, and may therefore be a contributing factor to periodontal disease and a potential indicator of risk.

Simultaneous profiling of sexually transmitted bacterial pathogens, microbiome, and concordant host response in cervical samples using whole transcriptome sequencing analysis

Catherine M. O’Connell1,#, Hayden Brochu2,#, Jenna Girardi1, Erin Harrell2, Aiden Jones2, Toni Darville1, Arlene C. Seña3 and Xinxia Peng2,4

This study used total RNA sequencing to analyze cervical samples from women at high risk for STIs, revealing that host transcriptional profiles can be linked to microbiome composition and STI infections, with implications for advancing our understanding of PID and identifying potential biomarkers.

Genome-wide analysis of yeast expression data based on a priori generated co-regulation cliques

Siyuan Sima1, Lukas Schmauder1 and Klaus Richter1

The study demonstrates the use of predefined genome-wide expression cliques, derived from extensive microarray data, to effectively analyze and visualize the complete gene expression response across various cellular conditions in yeast.

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Clonal spread and environmental persistence of carbapenem-resistant high-risk Pseudomonas aeruginosa in critical-care units of a Chilean national referral center for burn and trauma patients (2022)

July 20, 2026

This study highlight the circulation of high-risk P. aeruginosa clones in Chile and underscore the importance of molecular epidemiology in guiding infection control, optimizing antimicrobial therapy, and mitigating the clinical and economic burden of CRPA.

The transcriptome of dendritic cells redraws the boundaries between pathogenicity and commensalism in yeast

July 7, 2026

Our immune system meets many fungi, but reacts strongly to only a few. The comparison of key fungal species showed that responses differ mainly in timing and not just genes, highlighting the need to rethink what makes fungi harmful vs. harmless.

Yippee-like protein Moh1 links gene expression to metabolism and selective stress resistance in Saccharomyces cerevisiae

June 29, 2026

This study demonstrates that the conserved yeast protein Moh1 links metabolism, gene expression, and cell structure, thereby altering cell envelope permeability and leading to selective stress responses.

Fungistatic effect of sorbic acid on yeast cells via translational repression involving eIF2α phosphorylation and formation of Ded1- and eIF2B-granules

June 17, 2026

The findings of this study provide new insights into the physiological effects of sorbic acid on yeast cells, specifically regarding the regulation of translation-related factors.

Occlusal types shape oral microbiome stomatotypes and metabolic landscapes: A multi-omics perspective on host-microbe interaction

June 16, 2026

This study reveals how occlusal types shape oral microbiome “stomatotypes” and metabolic profiles in adolescents. It offers fresh insights that host anatomy drives microecology which may be associated with personalized oral health.

Genomic epidemiology of carbapenemase-producing Klebsiella pneumoniae circulating in a Chilean tertiary-care hospital (2021–2022): Molecular characterization, resistance-virulence convergence, and clinical associations

May 21, 2026

This study characterized carbapenem-resistant Kp (CR-Kp) strains isolated at the Hospital Clínico Universidad de Chile (HCUCH) 2021-2022 and explored associations with clinical characteristics.

Sugar-induced cell death (SICD) in Saccharomyces cerevisiae: insights into nitrogen-mediated rescue and apoptotic cell death pathways

May 11, 2026

This study examined mechanisms through which yeast sugar-induced cell death can be prevented. High concentrations of glucose induced a catastrophic response that was only rescued by highly preferred nitrogen sources and by preventing nuclear localization of specific cell death proteins.

TOR-dependent regulation of the yeast homolog of the juvenile Batten Disease-associated gene CLN3

March 11, 2026

This study identifies conditions and genes that induce BTN1 expression in yeast. We show that BTN1 expression is regulated by translational control and by the mTOR1 pathway. An understanding of when and why BTN1 expression will aid in understanding the expression of CLN3, which may be helpful in the treatment of this devastating disease.

Metagenomic and microbiological analyses of historical manuscripts for bacterial community profiling and bacteria-related biodeterioration assessment

March 1, 2026

By documenting both culturable and non-culturable taxa, this work provides a foundational dataset for understanding bacterial contributions to manuscript stability and offers a methodological framework for future research on biodeterioration dynamics in Islamic and global documentary heritage.

Overcoming phagocytosis resistance of hypervirulent Klebsiella pneumoniae by directly targeting capsules

February 16, 2026

This study highlights a promising strategy for disarming hypervirulent K. pneumoniae by directly targeting its key virulence factors and provides novel insights into antibacterial therapeutic approaches against this clinically significant pathogen.

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