Research Articles

Rewiring yeast acetate metabolism through MPC1 loss of function leads to mitochondrial damage and decreases chronological lifespan

Ivan Orlandi^1,2, Damiano Pellegrino Coppola² and Marina Vai^1,2

This work shows that MPC1-deficient cells make up for their impairment in mitochondrial pyruvate with a metabolic rewiring which involves several intermediates of the mitochondrially localized TCA cycle and the cytosolic glyoxylate shunt but ultimately results in a pro-aging process.

Overexpression of the transcription factor Yap1 modifies intracellular redox conditions and enhances recombinant protein secretion

Marizela Delic^1,2, Alexandra B. Graf^2,3, Gunda Koellensperger^1,4, Christina Haberhauer-Troyer^1,4, Stephan Hann^1,4, Diethard Mattanovich^1,2, Brigitte Gasser^1,2

This article investigates the role of Yap1 during the production of recombinant secretory proteins in glucose based growth conditions in Pichia pastoris, and reports a novel role of Yap1 during ER-resident oxidative protein folding.

Functional analysis of lipid metabolism genes in wine yeasts during alcoholic fermentation at low temperature

María López-Malo^1,2, Estéfani García-Ríos¹, Rosana Chiva¹ and José Manuel Guillamon¹

This study confirms the importance of specific genes in growth and fermentation activity of Saccharomyces cerevisiae at low temperature.

Angiotensin II type 1 receptor blockers increase tolerance of cells to copper and cisplatin

Pieter Spincemaille^1,+, Gursimran Chandhok^2,+, Andree Zibert², Hartmut Schmidt², Jef Verbeek³, Patrick Chaltin^4,5, Bruno P.A. Cammue^1,6,#, David Cassiman³, Karin Thevissen^1,#

This study reports the identification of the drug class of Angiotensin II Type 1 receptor blockers (ARBs) and shows that specific ARBs increase yeast tolerance to Cu and Cp, and affect markers of Cu-induced apoptosis. Likewise, this study finds that specific ARBs increase human cell line tolerance to Cu and decrease the prevalence of apoptotic markers.

An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids

Harriet Allison¹, Amanda J. O’Reilly¹, Jeremy Sternberg² and Mark C. Field¹

This work describes a novel family of type I membrane proteins ("invariant glycoproteins") and proposes them as trypanosomatid-specific ER-localised glycoproteins, with potential contributions to life cycle progression and immunity, that utilise oligomerisation as an ER retention mechanism.

Time resolved DNA occupancy dynamics during the respiratory oscillation uncover a global reset point in the yeast growth program

Cornelia Amariei, Rainer Machné, Viktor Stolc, Tomoyoshi Soga, Masaru Tomita and Douglas B. Murray

Using multiple approaches, this work implies a nucleosome focusing event as a key step that resets transcription during the respiratory oscillation.

Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae

António Rego^#, Ana Marta Duarte^#, Flávio Azevedo^#, Maria João Sousa, Manuela Côrte-Real and Susana R. Chaves

This work characterizes the involvement of MAPK signaling pathways in cell death induced by acetic acid in Saccharomyces cerevisiae.

Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death in Neurospora crassa

A. Pedro Gonçalves^1,2, João Monteiro², Chiara Lucchi², David J. Kowbel³, J. Miguel Cordeiro^1,4, Paulo Correia-de-Sá^1,4, Daniel J. Rigden⁵, N. Louise Glass³, Arnaldo Videira^1,2

The results presented here reveal that in Neurospora crassa, extracellular Ca2+ modulates cell death and the transcriptional alterations induced by staurosporine, and lead to the identification of two novel putative Ca2+-binding proteins, encoded by the NCU08524 and NCU06607 genes.

Multiple metabolic requirements for size homeostasis and initiation of division in Saccharomyces cerevisiae

Shivatheja Soma, Kailu Yang, Maria I. Morales and Michael Polymenis

This article reveals an unexpected diversity in the G1 cell cycle phenotypes of metabolic and biosynthetic Saccharomyces cerevisiae mutants, suggesting that growth requirements for cell division are multiple, distinct and imposed throughout the G1 phase of the cell cycle.

Previous Next

Two distinct and competitive pathways confer the cellcidal actions of artemisinins

January 1, 2015

The biological actions of artemisinin (ART), an antimalarial drug derived from Artemisia annua, remain poorly understood and controversial. This article concludes that ARTs are endowed with two major and distinct types of properties: a potent and specific mitochondria-dependent reaction and a more general and less specific heme-mediated reaction. The competitive nature of these two actions could be explained by their shared source of the consumable ARTs, so that inhibition of the heme-mediated degradation pathway would enable more ARTs to be available for the mitochondrial action. These properties of ARTs can be used to interpret the divergent antimalarial and anticancer actions of ARTs.

Measurement of apoptosis by SCAN©, a system for counting and analysis of fluorescently labelled nuclei

November 26, 2014

This work reports on a system for analyses of apoptosis-like programmed cell death in fungal hyphae that is composed of several modules, which enable automatic quantification of nuclei with chromatin condensation and DNA strand break in large datasets according to nuclei-associated fluorescent markers.