Research, Research Articles
Microbiota and metabolome dynamics induced by Shiga toxin-producing E. coli in an in vitro model of an infant’s colon
Mariana Izquierdo1,a, Deborah O’Sullivan2,a, Ophélie Uriot2, Morgane Brun2, Claude Durif2, Sylvain Denis2, Pablo Gallardo1, Cormac G M Gahan3-5, Lucie Etienne-Mesmin2, Stéphanie Blanquet-Diot2,b and Mauricio J. Farfan1.b
This study provides new evidence of the impact of EHEC in the microbiota and metabolome dynamics in an in vitro gut model that could be useful in understanding their physiopathology in this at-risk population, considering inter-individual variabilities in gut microbiota.
Ampicillin treatment in persister cell studies may cause non-physiological artifacts
Michel Fasnacht1,2, Hena Comic1,2, Isabella Moll1,2
This study shows at the example of L2 how insufficient purification of ampicillin persister cells can lead to the generation of non-physiological artifacts and provides a novel tool to improve the removal of residual cell debris.
Clostridium scindens promotes gallstone formation by inducing intrahepatic neutrophil extracellular traps through CXCL1 produced by colonic epithelial cells
Wenchao Yao1,a, Yuanhang He2,3,a, Zhihong Xie2,3, Qiang Wang2,3, Yang Chen2,4, Jingjing Yu2,3, Xuxu Liu2,3, Dongbo Xue2,3 , Liyi Wang2,3 and Chenjun Hao2,3
Through in vivo and in vitro experiments, we validated the reliability of C. scindens stimulating colonic epithelial cells to produce TLR2, activating the NF-κB signaling pathway, promoting CXCL1 expres-sion, and inducing intrahepatic neutrophil NETosis, which may be associated with gallstone formation.
Integrative Omics reveals changes in the cellular landscape of peroxisome-deficient pex3 yeast cells
Tjasa Kosir1,a, Hirak Das2,a, Marc Pilegaard Pedersen1, Ann-Kathrin Richard2, Marco Anteghini3,4, Vitor Martins dos Santos4,5, Silke Oeljeklaus2, Ida J. van der Klei1 and Bettina Warscheid2
To uncover the consequences of peroxisome deficiency, we compared Saccharomyces cerevisiae wild-type with pex3 cells, which lack peroxisomes, employing quantitative proteomics and transcriptomics technologies.
Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy
Moritz Mayera, Christina Schuga, Stefan Geimer, Till Klecker and Benedikt Westermann
Budding yeast Saccharomyces cerevisiae is widely used as a model organism to study the biogenesis and architecture of organellar membranes, which can be visualized by transmission electron microscopy (TEM).
A complex remodeling of cellular homeostasis distinguishes RSV/SARS-CoV-2 co-infected A549-hACE2 expressing cell lines
Claudia Vanetti1, Irma Saulle1,2, Valentina Artusa1,2, Claudia Moscheni1, Gioia Cappelletti1, Silvia Zecchini1, Sergio Strizzi1, Micaela Garziano1,2, Claudio Fenizia1,2, Antonella Tosoni1, Martina Broggiato1, Pasquale Ogno1, Manuela Nebuloni1, Mario Clerici2,3, Daria Trabattoni1, Fiona Limanaqi1 and Mara Biasin1
Given the common tropism of SARS-CoV-2 and RSV, and the unclear consequences of their mutual influence, we developed an in vitro lung epithelial cell model to study the molecular mechanisms and cellular pathways modulated in viral co-infection.
RidA proteins contribute to fitness of S. enterica and E. coli by reducing 2AA stress and moderating flux to isoleucine biosynthesis
Ronnie L. Fulton, Bryce R. Sawyer and Diana M Downs
This study solidifies the established role of RidA in removing 2AA, while also presenting evidence for a role of RidA in enhancing flux towards isoleucine biosynthesis in E. coli. Overall, these data emphasize that metabolic networks can generate distinct responses to perturbation, even when the individual components are conserved.
Fecal gelatinase does not predict mortality in patients with alcohol-associated hepatitis
Yongqiang Yang1,a, Philipp Hartmann2,3,a and Bernd Schnabl1,4
This study aimed to investigate the significance of fecal gelatinase on clinical outcomes in patients with alcohol-associated hepatitis. In conclusion, in our cohort, fecal gelatinase does not predict mortality and does not indicate higher disease severity in patients with alcohol-associated hepatitis.
Direct detection of stringent alarmones (pp)pGpp using malachite green
Muriel Schicketanz1, Magdalena Petrová2, Dominik Rejman2, Margherita Sosio3, Stefano Donadio3 and Yong Everett Zhang1
In this study, we demonstrate the surprising discovery of a commercially available, low-cost malachite green (MG) detection kit, originally designed for orthophosphate (Pi) detection, for detecting (p)ppGpp and its analogues, especially pGpp
At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations
May 20, 2014
The data presented in this work indicate that in H. polymorpha at neutral pH the chronological lifespan invariably extends upon increasing the carbon source concentration.
Salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA
March 3, 2014
In this work, the authors report that salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA (ρ0) and that this damage is related to elevated levels of SCW11 and salt stress-induced reactive oxygen species.
Protein oxidation in the intermembrane space of mitochondria is substrate-specific rather than general
March 3, 2014
In this work, the authors suggest that in Saccharomyces cerevisiae, the Mia40-dependent oxidation of proteins in the intermembrane space only takes place in specific proteins and presumably relies on the presence of Mia40-binding sites.
Reduced TORC1 signaling abolishes mitochondrial dysfunctions and shortened chronological lifespan of Isc1p-deficient cells
January 5, 2014
Overall, this article shows that the TORC1-Sch9p axis is deregulated in Isc1p-deficient Saccharomyces cerevisiae cells, contributing to mitochondrial dysfunction, enhanced oxidative stress sensitivity and premature aging of isc1Δ cells.
Tracking autophagy during proliferation and differentiation of Trypanosoma brucei
December 26, 2013
This article provides insights into the function of autophagy, a cellular degradation and recycling pathway, in the protozoan parasite Trypanosoma brucei.