Research, Research Articles
A time-resolved high-throughput screening of fission yeast deletion mutants for oxidative stress resistance
Mohammadtaha Pirsalehi, Rowshan Ara Islam, Kristal Ng, Olga Xintarakou, Peter Harold Thorpe and Charalampos Rallis
By capturing dynamic trajectories rather than static outcomes, this study exposes hidden layers of growth under oxidative stress and identifies new genetic determinants of cellular resilience in fission yeast.
Clonal spread and environmental persistence of carbapenem-resistant high-risk Pseudomonas aeruginosa in critical-care units of a Chilean national referral center for burn and trauma patients (2022)
Camila Ibarra1, Gustavo Araya2, Alhejandra Alvarez2, Leonardo Enríquez2, Carolina Arellano2, Fabricio Alarcón1, Constanza Fernández1, Claudio Vargas1, Rodrigo Vera1 and Roberto M. Vidal2
This study highlight the circulation of high-risk P. aeruginosa clones in Chile and underscore the importance of molecular epidemiology in guiding infection control, optimizing antimicrobial therapy, and mitigating the clinical and economic burden of CRPA.
The transcriptome of dendritic cells redraws the boundaries between pathogenicity and commensalism in yeast
Lisa Rizzetto1, Irene Stefanini2, Stefano Nenciarini3, Monica Di Paola3, Samantha Riccadonna1, Ivo G. Gut4,5, Marta Gut4,5, Simon Heath4,5 and Duccio Cavalieri3
Our immune system meets many fungi, but reacts strongly to only a few. The comparison of key fungal species showed that responses differ mainly in timing and not just genes, highlighting the need to rethink what makes fungi harmful vs. harmless.
Yippee-like protein Moh1 links gene expression to metabolism and selective stress resistance in Saccharomyces cerevisiae
Çağla Ece Olgun1,a, Gizem Turan Duman1,a, Gizem Güpür1, Hamit İzgi1, Mariam Huda2, Demet Çetin3, Zekiye Suludere4, Fatma Küçük Baloğlu5, Ayşe Koca Çaydaşı2 and Mesut Muyan1
This study demonstrates that the conserved yeast protein Moh1 links metabolism, gene expression, and cell structure, thereby altering cell envelope permeability and leading to selective stress responses.
Fungistatic effect of sorbic acid on yeast cells via translational repression involving eIF2α phosphorylation and formation of Ded1- and eIF2B-granules
Haruka Yoshiyama1, Wataru Nomura2 and Shingo Izawa1
The findings of this study provide new insights into the physiological effects of sorbic acid on yeast cells, specifically regarding the regulation of translation-related factors.
Occlusal types shape oral microbiome stomatotypes and metabolic landscapes: A multi-omics perspective on host-microbe interaction
Yufeng Duan1,a, Zheng Liu2,3,a, Wenting Lu1,a, Ning Zhao1, Lingjun Yuan1, Zhenxia Li1, Ting Zhou1, Shengzhao Xiao1, Dian Jing1, Xiaowen Zheng1, Wentao Shi4, Chao Liu1,*, Haixia Lu5,*, Qiang Feng6,* and Bing Fang1,*
This study reveals how occlusal types shape oral microbiome “stomatotypes” and metabolic profiles in adolescents. It offers fresh insights that host anatomy drives microecology which may be associated with personalized oral health.
Genomic epidemiology of carbapenemase-producing Klebsiella pneumoniae circulating in a Chilean tertiary-care hospital (2021–2022): Molecular characterization, resistance-virulence convergence, and clinical associations
Gustavo Araya1,2, Alhejandra Álvarez1, Carolina Arellano1, Rodrigo Bravo3, Abraham Gajardo4,5, Boris Barrera6, Francisco Silva6 and Roberto M. Vidal1
This study characterized carbapenem-resistant Kp (CR-Kp) strains isolated at the Hospital Clínico Universidad de Chile (HCUCH) 2021-2022 and explored associations with clinical characteristics.
Sugar-induced cell death (SICD) in Saccharomyces cerevisiae: insights into nitrogen-mediated rescue and apoptotic cell death pathways
Raveena Parbhudayal and Hai-Ping Cheng
This study examined mechanisms through which yeast sugar-induced cell death can be prevented. High concentrations of glucose induced a catastrophic response that was only rescued by highly preferred nitrogen sources and by preventing nuclear localization of specific cell death proteins.
TOR-dependent regulation of the yeast homolog of the juvenile Batten Disease-associated gene CLN3
Vijaykumar Pillalamarri1, Samuel W.M. Gatesy1, Amanda E. Grassel1, Lucienna Wolf1, Justin P. Whalley2 and David M. Mueller1,*
This study identifies conditions and genes that induce BTN1 expression in yeast. We show that BTN1 expression is regulated by translational control and by the mTOR1 pathway. An understanding of when and why BTN1 expression will aid in understanding the expression of CLN3, which may be helpful in the treatment of this devastating disease.
Fecal gelatinase does not predict mortality in patients with alcohol-associated hepatitis
August 26, 2024
This study aimed to investigate the significance of fecal gelatinase on clinical outcomes in patients with alcohol-associated hepatitis. In conclusion, in our cohort, fecal gelatinase does not predict mortality and does not indicate higher disease severity in patients with alcohol-associated hepatitis.
Promoter methylation and increased expression of PD-L1 in patients with active tuberculosis
July 29, 2024
The PD-1/PD-L1 pathway plays a pivotal role in T cell activity and is involved in the pathophysiology of tuberculosis. Here we show that PD-L1 expression is increased in patients with active tuberculosis and is correlated with treatment outcomes.
Quantification methods of Candida albicans are independent irrespective of fungal morphology
July 26, 2024
Our study demonstrated that the quantification methods of C. albicans (cells/mL, CFU/mL, and vPCR) did not agree, regardless of the fungal morphology/growth, even though a significant and strong correlation is observed.
Pathogenic Escherichia coli change the adhesion between neutrophils and endotheliocytes in the experimental bacteremia model
July 22, 2024
In this work, we have demonstrated that in the model of experimental septicemia there is a disruption of adhesion contacts between neutrophils and endothelial cells, manifested by a decrease in adhesion force and work upon exposure to E. coli.
Arsenite treatment induces Hsp90 aggregates distinct from conventional stress granules in fission yeast
July 19, 2024
Given the conserved role of Hsp90 as a molecular chaperone protein, our findings presented in this study may suggest a novel type of arsenite-induced biological condensates, wherein Hsp90 plays a key role in maintaining its integrity.
Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome
May 22, 2024
In this investigation, we unveiled a novel functional role of the major nuclear 3′→5′ exoribonuclease, Rrp6p, and its cofactor Rrp47p in the degradation of polyadenylated versions of several mature sncRNAs, including 5S, 5.8S rRNAs, all sn- and some select snoRNAs in the baker’s yeast S. cerevisiae.
Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches
May 16, 2024
In this work we set out to explore the relationship between the subcellular localization and regulation of kinases in the context of carbon source signaling. The data presented in this paper reinforce the notion that not only the activation/inactivation of kinases but also their subcellular localization and that of their targets influence fate decisions in response to environmental changes.
A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae
April 30, 2024
Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs. We established a MoClo toolkit and exemplified its application for the production of recombinant proteins in L. tarentolae.