Microreviews, Review

VAMP8 mucin exocytosis attenuates intestinal pathogenesis by Entamoeba histolytica

Steve Cornick1, France Moreau1, Herbert Y. Gaisano2, Kris Chadee1

This article comments on work published by Cornick et al. (mBio, 2017), which nominates SNARE-mediated exocytosis as the putative mechanism responsible for pathogen-induced mucus secretion from goblet cells.

Shutdown of interferon signaling by a viral-hijacked E3 ubiquitin ligase

Kaitlin A. Davis1 and John T. Patton2

This article comments on work published by Davis et al. (mBio, 2017), which describes molecular requirements that govern NSP1 recognition of β-TrCP, including an essential degron phosphorylation event, and the step-wise incorporation of NSP1 into hijacked cullin-RING E3 ligases (CRLs) that ubiquitinate and tag β-TrCP for degradation.

Breaking the bad: Bacillus blocks fungal virulence factors

François L. Mayer1 and James W. Kronstad1

This article comments on work published by Mayer & Kronstad (mBio, 2017), which identified the soil bacterium, Bacillus safensis as a potent inhibitor of virulence factor production by two major fungal pathogens of humans, Cryptococcus neoformans, and Candida albicans.

The integrated stress response in budding yeast lifespan extension

Spike D.L. Postnikoff1, Jay E. Johnson2 and Jessica K. Tyler1

 

This article summarizes how the budding yeast Saccharomyces cerevisiae has been instrumental in unraveling the molecular and cellular determinants of aging, and how the induction of cellular stress responses has been associated with experimental lifespan extension, thus underscoring the value of yeast as a model for developing potential aging therapies for humans.

Yeast for virus research

Richard Yuqi Zhao

This article summarizes the use of budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe) in virus research, highlighting their advantages for studying viral replication, interaction with host cells, and fundamental cellular processes affected by viruses, while discussing their potential in analyzing small viral genomes and facilitating the discovery of antiviral drugs.

Macrophages as drivers of an opportunistic infection

Annette C. Vergunst1, Nazareth Lopez Carranza1, Lili Zhang1,2, Margarida C. Gomes1, Yara Tasrini1,
Annemarie H. Meijer3 and David O’Callaghan1

This article comments on work published by Mesureur et al. (PloS Pathog, 2017), which shows that macrophages are essential for proliferation of B. cenocepacia in the host. This suggests a new paradigm for Bcc infections and urges the development of novel anti-infectious therapies to efficiently disarm these intrinsically antibiotic resistant facultative intracellular pathogens.

A yeast model for the mechanism of the Epstein-Barr virus immune evasion identifies a new therapeutic target to interfere with the virus stealthiness

María José Lista1, Rodrigo Prado Martins2, Gaelle Angrand1, Alicia Quillévéré1, Chrysoula Daskalogianni2, Cécile Voisset1, Marie-Paule Teulade-Fichou3, Robin Fåhraeus2 and Marc Blondel1

This article comments on a publication by Lista et al. (Nature Communications, 2017) that uncovered the role of the host cell nucleolin (NCL) in EBNA1 self-limitation of expression via a direct interaction of this protein with G-quadruplexes (G4) formed in GAr-encoding sequence of EBNA1 mRNA.

Exacerbating and reversing lysosomal storage diseases: from yeast to humans

Tamayanthi Rajakumar1, Andrew B. Munkacsi1,2 and Stephen L. Sturley3

This article summarizes the use of yeast models in advancing our understanding of lysosomal storage diseases (LSDs), where they have been instrumental in researching LSD mechanisms, screening for therapeutic compounds, and exploring genetic and gene-environment interactions relevant to diseases like Batten disease, cystinosis, and Niemann-Pick type C disease, as well as their connection to broader health issues such as viral infections and obesity.

Live fast, die fast principle in a single cell of fission yeast

Hidenori Nakaoka1

This article comments on a recent study (Nakaoka and Wakamoto, PLoS Biol, 2017), which developed a microfluidics-based platform to track multiple single cell lineages until death.

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A plant Bcl-2-associated athanogene is proteolytically activated to confer fungal resistance

April 16, 2016

This article comments on work published by Li et al. (Plant Cell, 2016), which focuses on the role of Bcl-2-associated athanogene 6 (BAG6) in plant innate immunity, showing that BAG6 plays a key role in basal plant defense against fungal pathogens.

The molecular and cellular action properties of artemisinins: what has yeast told us?

April 14, 2016

Artemisinin (ART) or Qinghaosu is a natural compound possessing superior anti-malarial activity. Although intensive studies have been done in the medicinal chemistry field to understand the structure-effect relationship, the biological actions of artemisinin are poorly understood and controversial. This review summarizes what we have learned from yeast about the basic biological properties of ARTs, as well as some key unanswered questions.

Metabolic network structure and function in bacteria goes beyond conserved enzyme components

April 14, 2016

This article comments on work published by Bazurto et al. (MBio, 2016), which demonstrated that conservation of metabolic components was not sufficient to predict network structure and function Escherichia coli.

Chemical proteomics approach reveals the direct targets and the heme-dependent activation mechanism of artemisinin in Plasmodium falciparum using an activity-based artemisinin probe

April 5, 2016

This article comments on work published by Wang et al. (Nat Commun, 2014), which provides insights into the mode-of-action of artemisinin and its specificity against malaria parasites.

Translational repression in malaria sporozoites

April 5, 2016

This article comments on work published by Zhang et al. (PLoS Pathog, 2016), which summarizea recent advances in the translational repression of gene expression in the malaria sporozoite.

Chromatin binding and silencing: Two roles of the same protein Lem2

April 4, 2016

This article comments on work published by Barrales et al. (Genes Dev, 2016), which identifies the nuclear envelope protein Lem2, a homolog of metazoan lamin-associated proteins (LAPs), as a relevant factor for heterochromatin silencing and perinuclear localization in the fission yeast Schizosaccharomyces pombe.

When and where? Pathogenic Escherichia coli differentially sense host D-serine using a universal transporter system to monitor their environment

March 31, 2016

This article comments on work published by Connolly et al. (PLoS Pathog, 2016), which describes the discovery of a functional and previously uncharacterized D-serine uptake system in E. coli.

Signaling pathways and posttranslational modifications of tau in Alzheimer’s disease: the humanization of yeast cells

March 27, 2016

In the past decade, yeast have been frequently employed to study the molecular mechanisms of human neurodegenerative diseases, generally by means of heterologous expression of genes encoding the relevant hallmark proteins. Substantial posttranslational modifications of many of these proteins are required for the development and progression of potentially disease relevant changes. We give an overview on common modifications as they occur in tau during AD and discuss potential approaches to humanize yeast in order to create modification patterns resembling the situation in mammalian cells.

The bacterial cell cycle checkpoint protein Obg and its role in programmed cell death

March 16, 2016

This article comments on work published by Dewachter et al. (mBio, 2015), which identified a programmed cell death mechanism in Escherichia coli that is triggered by a mutant isoform of the essential GTPase ObgE.

Bactericidal antibiotics induce programmed metabolic toxicity

March 9, 2016

This article comments on work published by Lobritz et al. (PNAS, 2015), which demonstrates that bactericidal antibiotics induce metabolic perturbations that are linked to and required for bactericidal antibiotic toxicity.

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