Promoter methylation and increased expression of PD-L1 in patients with active tuberculosis

July 29, 2024

The PD-1/PD-L1 pathway plays a pivotal role in T cell activity and is involved in the pathophysiology of tuberculosis. Here we show that PD-L1 expression is increased in patients with active tuberculosis and is correlated with treatment outcomes.

Quantification methods of Candida albicans are independent irrespective of fungal morphology

July 26, 2024

Our study demonstrated that the quantification methods of C. albicans (cells/mL, CFU/mL, and vPCR) did not agree, regardless of the fungal morphology/growth, even though a significant and strong correlation is observed.

Pathogenic Escherichia coli change the adhesion between neutrophils and endotheliocytes in the experimental bacteremia model

July 22, 2024

In this work, we have demonstrated that in the model of experimental septicemia there is a disruption of adhesion contacts between neutrophils and endothelial cells, manifested by a decrease in adhesion force and work upon exposure to E. coli.

Arsenite treatment induces Hsp90 aggregates distinct from conventional stress granules in fission yeast

July 19, 2024

Given the conserved role of Hsp90 as a molecular chaperone protein, our findings presented in this study may suggest a novel type of arsenite-induced biological condensates, wherein Hsp90 plays a key role in maintaining its integrity.

Unresolved mystery of cyclic nucleotide second messengers, periplasmic acid phosphatases and bacterial natural competence

July 18, 2024

In this study we aimed to identify the promotors responsible for the expression of the non-specific acid phosphatase AphA during different starvation conditions, to confirm the requirement of the cAMP-dependent CRP regulon for aphA expression, and to finally identify regulators of its expression.

Characterising glycosaminoglycans in human breastmilk and their potential role in infant health

July 4, 2024

Glycosaminoglycans are bioactive components present in breast milk and play a potential key role in determining infant health yet are overlooked by many contemporary studies. This review explores their relevance, use and characterisation techniques.

Neutralizing the threat: harnessing broadly neutralizing antibodies against HIV-1 for treatment and prevention

July 3, 2024

This review provides an overview of the advancements in HIV- 1-specific broadly neutralizing antibodies and discusses the insights gathered from recent clinical trials regarding their application in treating and preventing HIV-1 infection.

Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U- ExM protocols

June 21, 2024

Understanding cellular ultrastructure is tightly bound to microscopic resolution and the ability to identify individual components at that resolution. In this study we demonstrate mostly isotropic 4.5-fold expansion of several different compartments in Giardia cells and present an optimised, shortened, and modular protocol that can be swiftly adjusted to the investigators needs.

From microbes to medicine: harnessing the gut microbiota to combat prostate cancer

May 23, 2024

The gut microbiome (GM) has been identified as a crucial factor in the development and progression of various diseases, including cancer. This review highlights the important role that the GM may play in the development and progression of prostate cancer, through its influence on chronic inflammation, immune modulation, and other pathogenic mechanisms.

Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome

May 22, 2024

In this investigation, we unveiled a novel functional role of the major nuclear 3′→5′ exoribonuclease, Rrp6p, and its cofactor Rrp47p in the degradation of polyadenylated versions of several mature sncRNAs, including 5S, 5.8S rRNAs, all sn- and some select snoRNAs in the baker’s yeast S. cerevisiae.

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