A novel system to monitor mitochondrial translation in yeast
Authors:Tamara Suhm1, Lukas Habernig2, Magdalena Rzepka1, Jayasankar Mohanakrishnan Kaimal3, Claes Andréasson3, Sabrina Büttner2,3 and Martin Ott1
doi: 10.15698/mic2018.03.621
Volume 5, pp. 158 to 164, published 13/01/2018.
1 Department of Biochemistry and Biophysics, Stockholm University, SE-10691 Stockholm, Sweden.
2 Institute of Molecular Biosciences, University of Graz, A-8010 Graz, Austria.
3 Department of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden.
Keywords:
mitochondrial translation, flow cytometry, superfolder GFP, strain engineering
Corresponding Author(s):
Conflict of interest statement:
The authors declare no conflict of interest.
Please cite this article as:
Tamara Suhm, Lukas Habernig, Magdalena Rzepka, Jayasankar Mohanakrishnan Kaimal, Claes Andréasson, Sabrina Büttner and Martin Ott (2018). A novel system to monitor mitochondrial translation in yeast. Microbial Cell 5(3): 158-164. doi: 10.15698/mic2018.03.621
© 2018 Suhm et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
Abstract:
The mitochondrial genome is responsible for the production of a handful of polypeptides that are core subunits of the membrane-bound oxidative phosphorylation system. Until now the mechanistic studies of mitochondrial protein synthesis inside cells have been conducted with inhibition of cytoplasmic protein synthesis to reduce the background of nuclear gene expression with the undesired consequence of major disturbances of cellular signaling cascades. Here we have generated a system that allows direct monitoring of mitochondrial translation in unperturbed cells. A recoded gene for superfolder GFP was inserted into the yeast (Saccharomyces cerevisiae) mitochondrial genome and enabled the detection of translation through fluorescence microscopy and flow cytometry in functional mitochondria. This novel tool allows the investigation of the function and regulation of mitochondrial translation during stress signaling, aging and mitochondrial biogenesis.