Research, Research Reports
Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina
Matthias Wiemer and Heinz D. Osiewacz
In this article, paraquat is used to experimentally induce strong cellular oxidative stress in Podospora anserina wild-type cultures of different age, finding that this treatment has profound effects on gene expression, growth and lifespan.
Heat shock protein 90 and calcineurin pathway inhibitors enhance the efficacy of triazoles against Scedosporium prolificans via induction of apoptosis
Fazal Shirazi and Dimitrios P. Kontoyiannis
This article reports for the first time that posaconazole (PCZ) or itraconazolein (ICZ) in combination with the calcineurin inhibitor tacrolimus or the Hsp90 inhibitor 17-demethoxy-17-(2-propenylamino) geldanamycin renders S. prolificans exquisitely sensitive to PCZ or ICZ via apoptosis.
At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations
Adam Kawałek and Ida J. van der Klei
The data presented in this work indicate that in H. polymorpha at neutral pH the chronological lifespan invariably extends upon increasing the carbon source concentration.
Salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA
Qiuqiang Gao1, Liang-Chun Liou2, Qun Ren2, Xiaoming Bao3 and Zhaojie Zhang2
In this work, the authors report that salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA (ρ0) and that this damage is related to elevated levels of SCW11 and salt stress-induced reactive oxygen species.
Protein oxidation in the intermembrane space of mitochondria is substrate-specific rather than general
Valentina Peleh1, Jan Riemer2, Andrew Dancis3 and Johannes M. Herrmann1
In this work, the authors suggest that in Saccharomyces cerevisiae, the Mia40-dependent oxidation of proteins in the intermembrane space only takes place in specific proteins and presumably relies on the presence of Mia40-binding sites.
Deletion of AIF1 but not of YCA1/MCA1 protects Saccharomyces cerevisiae and Candida albicans cells from caspofungin-induced programmed cell death
Christopher Chin1,2,#, Faith Donaghey1,#, Katherine Helming1,3,#, Morgan McCarthy1,#, Stephen Rogers1, and Nicanor Austriaco1
This work suggests that deleting AIF1 but not YCA1/MCA1 protects S. cerevisiae and Candida albicans from caspofungin-induced cell death. This is not only the first time that AIF1 has been specifically tied to cell death in Candida but also the first time that caspofungin resistance has been linked to the cell death machinery in yeast.
Reduced TORC1 signaling abolishes mitochondrial dysfunctions and shortened chronological lifespan of Isc1p-deficient cells
Vitor Teixeira1,2, Tânia C. Medeiros1, Rita Vilaça1,2, Pedro Moradas-Ferreira1,2, and Vítor Costa1,2
Overall, this article shows that the TORC1-Sch9p axis is deregulated in Isc1p-deficient Saccharomyces cerevisiae cells, contributing to mitochondrial dysfunction, enhanced oxidative stress sensitivity and premature aging of isc1Δ cells.
Early manifestations of replicative aging in the yeast Saccharomyces cerevisiae.
Maksim I. Sorokin1,3, Dmitry A. Knorre2,3, and Fedor F. Severin2,3
The data preseted herein suggest that retrograde signaling starts to malfunction in relatively young cells, leading to accumulation of heterogeneous mitochondria within one cell. The latter may further contribute to a decline in stress resistances.
Tracking autophagy during proliferation and differentiation of Trypanosoma brucei
William R. Proto1, Nathaniel G. Jones1, Graham H. Coombs2, and Jeremy C. Mottram1
This article provides insights into the function of autophagy, a cellular degradation and recycling pathway, in the protozoan parasite Trypanosoma brucei.
A time-resolved high-throughput screening of fission yeast deletion mutants for oxidative stress resistance
July 22, 2026
By capturing dynamic trajectories rather than static outcomes, this study exposes hidden layers of growth under oxidative stress and identifies new genetic determinants of cellular resilience in fission yeast.
Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy
November 18, 2024
Budding yeast Saccharomyces cerevisiae is widely used as a model organism to study the biogenesis and architecture of organellar membranes, which can be visualized by transmission electron microscopy (TEM).
Direct detection of stringent alarmones (pp)pGpp using malachite green
August 5, 2024
In this study, we demonstrate the surprising discovery of a commercially available, low-cost malachite green (MG) detection kit, originally designed for orthophosphate (Pi) detection, for detecting (p)ppGpp and its analogues, especially pGpp
Unresolved mystery of cyclic nucleotide second messengers, periplasmic acid phosphatases and bacterial natural competence
July 18, 2024
In this study we aimed to identify the promotors responsible for the expression of the non-specific acid phosphatase AphA during different starvation conditions, to confirm the requirement of the cAMP-dependent CRP regulon for aphA expression, and to finally identify regulators of its expression.
Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2
August 8, 2023
We show here that apart from its effector role for Elongator-dependent tRNA modification in yeast, Kti13 alias Dph8 also operates in step one of the diphthamide modification pathway.
Atg1, a key regulator of autophagy, functions to promote MAPK activation and cell death upon calcium overload in fission yeast
May 10, 2023
Here, we provide evidence that the fission yeast Atg1 regulates cell death responses upon intracellular calcium load in addition to its role in promoting Pmk1 MAPK.
Investigating the role of G-quadruplexes at Saccharomyces cerevisiae telomeres
May 19, 2022
In this study, the authors examine the in vivo relevance of telomeric G-quadruplexes in the budding yeast Saccharomyces cerevisiae by expressing a mutant telomerase RNA subunit (tlc1-tm) that introduces mutant telomeric repeats instead to the distal ends of telomeres. (...)