Research – Page 9 – Microbial Cell

Research, Research Articles

Genome, transcriptome and secretome analyses of the antagonistic, yeast-like fungus Aureobasidium pullulans to identify potential biocontrol genes

Maria Paula Rueda-Mejia¹, Lukas Nägeli¹, Stefanie Lutz², Richard D. Hayes³, Adithi R. Varadarajan², Igor V. Grigoriev^3,4, Christian H. Ahrens^2,5 and Florian M. Freimoser¹

This study highlights the value of a sequential approach starting with genome mining and consecutive transcriptome and secretome analyses in order to identify a limited number of potential target genes for detailed, functional analyses in Aureobasidium pullulans.

Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast

Catalina A. Romila^1,#, StJohn Townsend^1,2,#, Michal Malecki^1,3, Stephan Kamrad^1,2,4, María Rodríguez-López¹, Olivia Hillson¹, Cristina Cotobal¹, Markus Ralser^2,5 and Jürg Bähler¹

This work presents two approaches to study chronological lifespan (CLS) for medium- to high-throughput applications, a method for Bar-seq to identify mutants showing altered CLS and a novel medium-throughput colony-forming units assay that can be largely automated by robotics.

Proanthocyanidin-enriched cranberry extract induces resilient bacterial community dynamics in a gnotobiotic mouse model

Catherine C. Neto^1,2,#, Benedikt M. Mortzfeld^3,#, John R. Turbitt^1,2, Shakti K. Bhattarai³, Vladimir Yeliseyev⁴, Nicholas DiBenedetto⁴, Lynn Bry⁴ and Vanni Bucci^2,3

This study investigates the effect of a water-soluble, proanthocyanidin-rich cranberry juice extract on the short-term dynamics of a human-derived bacterial community in a gnotobiotic mouse model.

Dry biocleaning of artwork: an innovative methodology for Cultural Heritage recovery?

Giancarlo Ranalli¹, Pilar Bosch-Roig², Simone Crudele¹, Laura Rampazzi^3,4, Cristina Corti³ and Elisabetta Zanardini⁵

This work proposes an innovative methodology based on applied biotechnology for the recovery of altered stonework: the "dry biocleaning", which envisages the use of dehydrated microbial cells without the use of free water or gel-based matrices.

Aeration mitigates endoplasmic reticulum stress in Saccharomyces cerevisiae even without mitochondrial respiration

Huong Thi Phuong¹, Yuki Ishiwata-Kimata¹, Yuki Nishi¹, Norie Oguchi¹, Hiroshi Takagi¹ and Yukio Kimata¹

This work demonstrates a scenario, in which aeration acts beneficially on Saccharmyces cerevisiae cells even under fermentative conditions.

A novel BR-SMAD is required for larval development in barber’s pole worm Haemonchus contortus

Fangfang Li¹, Peixi Qin¹, Lisha Ye¹, Nishith Gupta^1,2,3 and Min Hu¹

The herein presented results show a BMP-like receptor-regulated SMAD in Haemonchus contortus that is required for larval differentiation and underscore an adaptive functional repurposing of BMP-signaling in parasitic worms.

Nutrient sensing and cAMP signaling in yeast: G-protein coupled receptor versus transceptor activation of PKA

Griet Van Zeebroeck^1,2,†, Liesbeth Demuyser^1,2,†, Zhiqiang Zhang^1,2, Ines Cottignie^1,2 and Johan M. Thevelein^1,2

The herein presented work supports a model, in which nutrient transceptors are evolutionary ancestors of GPCRs, employing a more primitive direct signaling mechanism compared to the indirect cAMP second-messenger signaling mechanism used by GPCRs for activation of PKA.

Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts

Rintaro Tsuchikado^1,#, Satoshi Kami^1,#, Sawako Takahashi¹ and Hiromi Nishida¹

In this study Tsuchikado et al. show that DNA replication is crucial for plasma membrane biosynthesis and vacuole formation in Enterococcus faecalis protoplasts. Novobiocin inhibits DNA replication, blocking cell enlargement and vacuole formation. Extended treatment prevents re-enlargement after removal.

Variants of the human RAD52 gene confer defects in ionizing radiation resistance and homologous recombination repair in budding yeast

Alissa D. Clear^1,2,3, Glenn M. Manthey^1,2, Olivia Lewis^4,5, Isabelle Y. Lopez^4,6, Rossana Rico^4,7, Shannon Owens^8,9, M. Cristina Negritto¹⁰, Elise W. Wolf^10,11, Jason Xu^10,12, Nikola Kenjić¹³, J. Jefferson P. Perry¹³, Aaron W. Adamson¹⁴, Susan L. Neuhausen¹⁴, Adam M. Bailis^1,2,15

RAD52 is a key protein in DNA repair and suppresses DNA damage in yeast; however, certain variants affecting BRCA2 mutations fail to correct HRR defects. This suggests that HsRAD52 aids multiple DNA repair mechanisms and could be targeted for use in treating BRCA2-deficient cancers.

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Landscapes and bacterial signatures of mucosa-associated intestinal microbiota in Chilean and Spanish patients with inflammatory bowel disease

June 18, 2021

This study investigates the landscapes and alterations of mucosa-associated intestinal microbiota in patients with inflammatory bowel diseases, which cause chronic inflammation of the gut, including ulcerative colitis and Crohn’s disease.

Genome, transcriptome and secretome analyses of the antagonistic, yeast-like fungus Aureobasidium pullulans to identify potential biocontrol genes

June 8, 2021

Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast

May 10, 2021

Variants of the human RAD52 gene confer defects in ionizing radiation resistance and homologous recombination repair in budding yeast

July 20, 2020

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