Research – Page 9 – Microbial Cell

Research Articles

Measurement of apoptosis by SCAN©, a system for counting and analysis of fluorescently labelled nuclei

Neta Shlezinger^1,#, Elad Eizner^1,2,#, Stas Dubinchik², Anna Minz-Dub¹, Rachel Tetroashvili¹, Adi Reider¹, Amir Sharon¹

This work reports on a system for analyses of apoptosis-like programmed cell death in fungal hyphae that is composed of several modules, which enable automatic quantification of nuclei with chromatin condensation and DNA strand break in large datasets according to nuclei-associated fluorescent markers.

Rewiring yeast acetate metabolism through MPC1 loss of function leads to mitochondrial damage and decreases chronological lifespan

Ivan Orlandi^1,2, Damiano Pellegrino Coppola² and Marina Vai^1,2

This work shows that MPC1-deficient cells make up for their impairment in mitochondrial pyruvate with a metabolic rewiring which involves several intermediates of the mitochondrially localized TCA cycle and the cytosolic glyoxylate shunt but ultimately results in a pro-aging process.

Overexpression of the transcription factor Yap1 modifies intracellular redox conditions and enhances recombinant protein secretion

Marizela Delic^1,2, Alexandra B. Graf^2,3, Gunda Koellensperger^1,4, Christina Haberhauer-Troyer^1,4, Stephan Hann^1,4, Diethard Mattanovich^1,2, Brigitte Gasser^1,2

This article investigates the role of Yap1 during the production of recombinant secretory proteins in glucose based growth conditions in Pichia pastoris, and reports a novel role of Yap1 during ER-resident oxidative protein folding.

Functional analysis of lipid metabolism genes in wine yeasts during alcoholic fermentation at low temperature

María López-Malo^1,2, Estéfani García-Ríos¹, Rosana Chiva¹ and José Manuel Guillamon¹

This study confirms the importance of specific genes in growth and fermentation activity of Saccharomyces cerevisiae at low temperature.

Angiotensin II type 1 receptor blockers increase tolerance of cells to copper and cisplatin

Pieter Spincemaille^1,+, Gursimran Chandhok^2,+, Andree Zibert², Hartmut Schmidt², Jef Verbeek³, Patrick Chaltin^4,5, Bruno P.A. Cammue^1,6,#, David Cassiman³, Karin Thevissen^1,#

This study reports the identification of the drug class of Angiotensin II Type 1 receptor blockers (ARBs) and shows that specific ARBs increase yeast tolerance to Cu and Cp, and affect markers of Cu-induced apoptosis. Likewise, this study finds that specific ARBs increase human cell line tolerance to Cu and decrease the prevalence of apoptotic markers.

An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids

Harriet Allison¹, Amanda J. O’Reilly¹, Jeremy Sternberg² and Mark C. Field¹

This work describes a novel family of type I membrane proteins ("invariant glycoproteins") and proposes them as trypanosomatid-specific ER-localised glycoproteins, with potential contributions to life cycle progression and immunity, that utilise oligomerisation as an ER retention mechanism.

Time resolved DNA occupancy dynamics during the respiratory oscillation uncover a global reset point in the yeast growth program

Cornelia Amariei, Rainer Machné, Viktor Stolc, Tomoyoshi Soga, Masaru Tomita and Douglas B. Murray

Using multiple approaches, this work implies a nucleosome focusing event as a key step that resets transcription during the respiratory oscillation.

Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae

António Rego^#, Ana Marta Duarte^#, Flávio Azevedo^#, Maria João Sousa, Manuela Côrte-Real and Susana R. Chaves

This work characterizes the involvement of MAPK signaling pathways in cell death induced by acetic acid in Saccharomyces cerevisiae.

Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death in Neurospora crassa

A. Pedro Gonçalves^1,2, João Monteiro², Chiara Lucchi², David J. Kowbel³, J. Miguel Cordeiro^1,4, Paulo Correia-de-Sá^1,4, Daniel J. Rigden⁵, N. Louise Glass³, Arnaldo Videira^1,2

The results presented here reveal that in Neurospora crassa, extracellular Ca2+ modulates cell death and the transcriptional alterations induced by staurosporine, and lead to the identification of two novel putative Ca2+-binding proteins, encoded by the NCU08524 and NCU06607 genes.

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Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress

November 26, 2021

The budding yeast Saccharomyces cerevisiae is capable of surviving extreme water loss for a long time. However, less is known about the mechanism of its desiccation tolerance. In this study, we revealed that in an exponential culture, all desiccation tolerant yeast cells were in G1 phase and had condensed chromosomes. (...)

Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays

November 25, 2021

The early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections is required to identify and isolate contagious patients to prevent further transmission of SARS-CoV-2. In this study, we present a multitarget real-time TaqMan reverse transcription PCR (rRT-PCR) assay for the quantitative detection of SARS-CoV-2 and some of its circulating variants harboring mutations that give the virus a selective advantage. Seven different primer-probe sets that included probes containing locked nucleic acid (LNA) nucleotides were designed to amplify specific wild-type and mutant sequences in Orf1ab, Envelope (E), Spike (S), and Nucleocapsid (N) genes (...)

Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking

October 27, 2021

This work demonstrates that the yeast Synthetic Physical Interactions method is a rapid way to identify potential functions of ectopic viral proteins.

Airborne bacteria in show caves from Southern Spain

July 26, 2021

This study analyzes the factors conditioning the diversity of airborne bacteria recorded in three Andalusian show caves, subjected to different managements.

Landscapes and bacterial signatures of mucosa-associated intestinal microbiota in Chilean and Spanish patients with inflammatory bowel disease

June 18, 2021

This study investigates the landscapes and alterations of mucosa-associated intestinal microbiota in patients with inflammatory bowel diseases, which cause chronic inflammation of the gut, including ulcerative colitis and Crohn’s disease.

Genome, transcriptome and secretome analyses of the antagonistic, yeast-like fungus Aureobasidium pullulans to identify potential biocontrol genes

June 8, 2021

This study highlights the value of a sequential approach starting with genome mining and consecutive transcriptome and secretome analyses in order to identify a limited number of potential target genes for detailed, functional analyses in Aureobasidium pullulans.

Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast

May 10, 2021

This work presents two approaches to study chronological lifespan (CLS) for medium- to high-throughput applications, a method for Bar-seq to identify mutants showing altered CLS and a novel medium-throughput colony-forming units assay that can be largely automated by robotics.