Research – Page 9 – Microbial Cell

Research Articles

Dissecting the cell cycle regulation, DNA damage sensitivity and lifespan effects of caffeine in fission yeast

John-Patrick Alao¹, Juhi Kumar¹, Despina Stamataki² and Charalampos Rallis¹

Our findings show that caffeine accelerates mitotic division and is beneficial for CLS through AMPK. Direct pharmacological targeting of AMPK may serve towards healthspan and lifespan benefits beyond yeasts, given the highly conserved nature of this key regulatory cellular energy sensor.

An adenine model of inborn metabolism errors alters TDP-43 aggregation and reduces its toxicity in yeast revealing insights into protein misfolding diseases

Sangeun Park, Sei-Kyoung Park, Peter Blair and Susan W. Liebman

This work offers new insights into the potential interactions between me-tabolite-based amyloids and pathological protein aggregates, with broad implications for understanding protein misfolding diseases.

Microbiota and metabolome dynamics induced by Shiga toxin-producing <i>E. coli</i> in an <i>in vitro</i> model of an infant’s colon

Microbiota and metabolome dynamics induced by Shiga toxin-producing E. coli in an in vitro model of an infant’s colon

Mariana Izquierdo^1,a, Deborah O’Sullivan^2,a, Ophélie Uriot², Morgane Brun², Claude Durif², Sylvain Denis², Pablo Gallardo¹, Cormac G M Gahan^3-5, Lucie Etienne-Mesmin², Stéphanie Blanquet-Diot^2,b and Mauricio J. Farfan^1.b

This study provides new evidence of the impact of EHEC in the microbiota and metabolome dynamics in an in vitro gut model that could be useful in understanding their physiopathology in this at-risk population, considering inter-individual variabilities in gut microbiota.

Ampicillin treatment in persister cell studies may cause non-physiological artifacts

Michel Fasnacht^1,2, Hena Comic^1,2, Isabella Moll^1,2

This study shows at the example of L2 how insufficient purification of ampicillin persister cells can lead to the generation of non-physiological artifacts and provides a novel tool to improve the removal of residual cell debris.

<i>Clostridium scindens</i> promotes gallstone formation by inducing intrahepatic neutrophil extracellular traps through CXCL1 produced by colonic epithelial cells

Clostridium scindens promotes gallstone formation by inducing intrahepatic neutrophil extracellular traps through CXCL1 produced by colonic epithelial cells

Wenchao Yao^1,a, Yuanhang He^2,3,a, Zhihong Xie^2,3, Qiang Wang^2,3, Yang Chen^2,4, Jingjing Yu^2,3, Xuxu Liu^2,3, Dongbo Xue^2,3 , Liyi Wang^2,3 and Chenjun Hao^2,3

Through in vivo and in vitro experiments, we validated the reliability of C. scindens stimulating colonic epithelial cells to produce TLR2, activating the NF-κB signaling pathway, promoting CXCL1 expres-sion, and inducing intrahepatic neutrophil NETosis, which may be associated with gallstone formation.

Integrative Omics reveals changes in the cellular landscape of peroxisome-deficient <i>pex3</i> yeast cells

Integrative Omics reveals changes in the cellular landscape of peroxisome-deficient pex3 yeast cells

Tjasa Kosir^1,a, Hirak Das^2,a, Marc Pilegaard Pedersen¹, Ann-Kathrin Richard², Marco Anteghini^3,4, Vitor Martins dos Santos^4,5, Silke Oeljeklaus², Ida J. van der Klei¹ and Bettina Warscheid²

To uncover the consequences of peroxisome deficiency, we compared Saccharomyces cerevisiae wild-type with pex3 cells, which lack peroxisomes, employing quantitative proteomics and transcriptomics technologies.

A complex remodeling of cellular homeostasis distinguishes RSV/SARS-CoV-2 co-infected A549-hACE2 expressing cell lines

Claudia Vanetti¹, Irma Saulle^1,2, Valentina Artusa^1,2, Claudia Moscheni¹, Gioia Cappelletti¹, Silvia Zecchini¹, Sergio Strizzi¹, Micaela Garziano^1,2, Claudio Fenizia^1,2, Antonella Tosoni¹, Martina Broggiato¹, Pasquale Ogno¹, Manuela Nebuloni¹, Mario Clerici^2,3, Daria Trabattoni¹, Fiona Limanaqi¹ and Mara Biasin¹

Given the common tropism of SARS-CoV-2 and RSV, and the unclear consequences of their mutual influence, we developed an in vitro lung epithelial cell model to study the molecular mechanisms and cellular pathways modulated in viral co-infection.

RidA proteins contribute to ﬁtness of S. enterica and E. coli by reducing 2AA stress and moderating ﬂux to isoleucine biosynthesis

Ronnie L. Fulton, Bryce R. Sawyer and Diana M Downs

This study solidifies the established role of RidA in removing 2AA, while also presenting evidence for a role of RidA in enhancing ﬂux towards isoleucine biosynthesis in E. coli. Overall, these data emphasize that metabolic networks can generate distinct responses to perturbation, even when the individual components are conserved.

Fecal gelatinase does not predict mortality in patients with alcohol-associated hepatitis

Yongqiang Yang^1,a, Philipp Hartmann^2,3,a and Bernd Schnabl^1,4

This study aimed to investigate the significance of fecal gelatinase on clinical outcomes in patients with alcohol-associated hepatitis. In conclusion, in our cohort, fecal gelatinase does not predict mortality and does not indicate higher disease severity in patients with alcohol-associated hepatitis.

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Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress

November 26, 2021

The budding yeast Saccharomyces cerevisiae is capable of surviving extreme water loss for a long time. However, less is known about the mechanism of its desiccation tolerance. In this study, we revealed that in an exponential culture, all desiccation tolerant yeast cells were in G1 phase and had condensed chromosomes. (...)

Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays

November 25, 2021

The early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections is required to identify and isolate contagious patients to prevent further transmission of SARS-CoV-2. In this study, we present a multitarget real-time TaqMan reverse transcription PCR (rRT-PCR) assay for the quantitative detection of SARS-CoV-2 and some of its circulating variants harboring mutations that give the virus a selective advantage. Seven different primer-probe sets that included probes containing locked nucleic acid (LNA) nucleotides were designed to amplify specific wild-type and mutant sequences in Orf1ab, Envelope (E), Spike (S), and Nucleocapsid (N) genes (...)

Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking

October 27, 2021

This work demonstrates that the yeast Synthetic Physical Interactions method is a rapid way to identify potential functions of ectopic viral proteins.

Airborne bacteria in show caves from Southern Spain

July 26, 2021

This study analyzes the factors conditioning the diversity of airborne bacteria recorded in three Andalusian show caves, subjected to different managements.

Landscapes and bacterial signatures of mucosa-associated intestinal microbiota in Chilean and Spanish patients with inflammatory bowel disease

June 18, 2021

This study investigates the landscapes and alterations of mucosa-associated intestinal microbiota in patients with inflammatory bowel diseases, which cause chronic inflammation of the gut, including ulcerative colitis and Crohn’s disease.

Genome, transcriptome and secretome analyses of the antagonistic, yeast-like fungus Aureobasidium pullulans to identify potential biocontrol genes

June 8, 2021

This study highlights the value of a sequential approach starting with genome mining and consecutive transcriptome and secretome analyses in order to identify a limited number of potential target genes for detailed, functional analyses in Aureobasidium pullulans.

Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast

May 10, 2021

This work presents two approaches to study chronological lifespan (CLS) for medium- to high-throughput applications, a method for Bar-seq to identify mutants showing altered CLS and a novel medium-throughput colony-forming units assay that can be largely automated by robotics.