Research – Page 9 – Microbial Cell

Research Articles

Promoter methylation and increased expression of PD-L1 in patients with active tuberculosis

Yen-Han Tseng^1,2, Sheng-Wei Pan^1,2,3, Jhong-Ru Huang^2,4, Chang-Ching Lee¹, Jung-Jyh Hung^2,5, Po-Kuei Hsu^2,5, Nien-Jung Chen⁶, Wei-Juin Su^2,7, Yuh-Min Chen^1,2 and Jia-Yih Feng^1,2,8

The PD-1/PD-L1 pathway plays a pivotal role in T cell activity and is involved in the pathophysiology of tuberculosis. Here we show that PD-L1 expression is increased in patients with active tuberculosis and is correlated with treatment outcomes.

Quantiﬁcation methods of Candida albicans are independent irrespective of fungal morphology

Amanda B Soares¹, Maria C de Albuquerque¹, Leticia M Rosa¹, Marlise I Klein ², Ana C Paravina¹, Paula A Barbugli¹, Livia N Dovigo³ and Ewerton G de O Mima¹

Our study demonstrated that the quantification methods of C. albicans (cells/mL, CFU/mL, and vPCR) did not agree, regardless of the fungal morphology/growth, even though a significant and strong correlation is observed.

Pathogenic Escherichia coli change the adhesion between neutrophils and endotheliocytes in the experimental bacteremia model

Svetlana N Pleskova^1,2,*, Nikolay A Bezrukov¹, Sergey Z Bobyk¹, Ekaterina N Gorshkova¹ and Dimitri V Novikov³

In this work, we have demonstrated that in the model of experimental septicemia there is a disruption of adhesion contacts between neutrophils and endothelial cells, manifested by a decrease in adhesion force and work upon exposure to E. coli.

Arsenite treatment induces Hsp90 aggregates distinct from conventional stress granules in ﬁssion yeast

Naofumi Tomimoto^a, Teruaki Takasaki^a and Reiko Sugiura

Given the conserved role of Hsp90 as a molecular chaperone protein, our findings presented in this study may suggest a novel type of arsenite-induced biological condensates, wherein Hsp90 plays a key role in maintaining its integrity.

Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U- ExM protocols

Clirim Jetishi^1,2,a,Erina A. Balmer^1,2,a, Bianca M. Berger^1,2,a, Carmen Faso^1,3,4and Torsten Ochsenreiter¹

Understanding cellular ultrastructure is tightly bound to microscopic resolution and the ability to identify individual components at that resolution. In this study we demonstrate mostly isotropic 4.5-fold expansion of several different compartments in Giardia cells and present an optimised, shortened, and modular protocol that can be swiftly adjusted to the investigators needs.

Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome

Anusha Chaudhuri^1,#, Soumita Paul^2,#, Mayukh Banerjea² and Biswadip Das²

In this investigation, we unveiled a novel functional role of the major nuclear 3′→5′ exoribonuclease, Rrp6p, and its cofactor Rrp47p in the degradation of polyadenylated versions of several mature sncRNAs, including 5S, 5.8S rRNAs, all sn- and some select snoRNAs in the baker’s yeast S. cerevisiae.

Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches

Svenja Braam¹, Farida Tripodi², Linnea Österberg^1,3, Sebastian Persson¹, Niek Welkenhuysen¹, Paola Coccetti² and Marija Cvijovic¹

In this work we set out to explore the relationship between the subcellular localization and regulation of kinases in the context of carbon source signaling. The data presented in this paper reinforce the notion that not only the activation/inactivation of kinases but also their subcellular localization and that of their targets influence fate decisions in response to environmental changes.

A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae

Katrin Hieronimus^1,2,#, Tabea Donauer^1,2,#, Jonas Klein^1,#, Bastian Hinkel^1,#, Julia Vanessa Spänle^1,#, Anna Probst^1,#, Justus Niemeyer^1,#, Salina Kibrom¹, Anna Maria Kiefer¹, Luzia Schneider², Britta Husemann², Eileen Bischoff², Sophie Möhring², Nicolas Bayer¹, Dorothée Klein¹, Adrian Engels¹, Benjamin Gustav Ziehmer², Julian Stieß³, Pavlo Moroka¹, Michael Schroda¹, and Marcel Deponte²

Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs. We established a MoClo toolkit and exemplified its application for the production of recombinant proteins in L. tarentolae.

A metagenomic approach to unveil the association between fecal gut microbiota and short-chain fatty acids in diarrhea caused by diarrheagenic Escherichia coli in children

Pablo Gallardo^1,2, Mariana Izquierdo², Tomeu Viver³, Esteban Bustos-Caparros³, Dana Piras², Roberto M. Vidal⁴, Hermie J.M. Harmsen¹ and Mauricio J. Farfan²

Diarrheagenic Escherichia coli (DEC) is the main cause of diarrhea in children under five years old. The virulence of DEC is tightly regulated by environmental signals influenced by the gut microbiota and its metabolites. Our results increase the knowledge of the association between short chain fatty acids during diarrhea and changes in the microbiota composition associated with the presence of DEC pathogens.

Previous Next

Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress

November 26, 2021

The budding yeast Saccharomyces cerevisiae is capable of surviving extreme water loss for a long time. However, less is known about the mechanism of its desiccation tolerance. In this study, we revealed that in an exponential culture, all desiccation tolerant yeast cells were in G1 phase and had condensed chromosomes. (...)

Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays

November 25, 2021

The early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections is required to identify and isolate contagious patients to prevent further transmission of SARS-CoV-2. In this study, we present a multitarget real-time TaqMan reverse transcription PCR (rRT-PCR) assay for the quantitative detection of SARS-CoV-2 and some of its circulating variants harboring mutations that give the virus a selective advantage. Seven different primer-probe sets that included probes containing locked nucleic acid (LNA) nucleotides were designed to amplify specific wild-type and mutant sequences in Orf1ab, Envelope (E), Spike (S), and Nucleocapsid (N) genes (...)

Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking

October 27, 2021

This work demonstrates that the yeast Synthetic Physical Interactions method is a rapid way to identify potential functions of ectopic viral proteins.

Airborne bacteria in show caves from Southern Spain

July 26, 2021

This study analyzes the factors conditioning the diversity of airborne bacteria recorded in three Andalusian show caves, subjected to different managements.

Landscapes and bacterial signatures of mucosa-associated intestinal microbiota in Chilean and Spanish patients with inflammatory bowel disease

June 18, 2021

This study investigates the landscapes and alterations of mucosa-associated intestinal microbiota in patients with inflammatory bowel diseases, which cause chronic inflammation of the gut, including ulcerative colitis and Crohn’s disease.

Genome, transcriptome and secretome analyses of the antagonistic, yeast-like fungus Aureobasidium pullulans to identify potential biocontrol genes

June 8, 2021

This study highlights the value of a sequential approach starting with genome mining and consecutive transcriptome and secretome analyses in order to identify a limited number of potential target genes for detailed, functional analyses in Aureobasidium pullulans.

Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast

May 10, 2021

This work presents two approaches to study chronological lifespan (CLS) for medium- to high-throughput applications, a method for Bar-seq to identify mutants showing altered CLS and a novel medium-throughput colony-forming units assay that can be largely automated by robotics.