Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches

Authors:

Svenja Braam1, Farida Tripodi2, Linnea Österberg1,3, Sebastian Persson1, Niek Welkenhuysen1, Paola Coccetti2 and Marija Cvijovic1

doi: 10.15698/mic2024.05.822
Volume 11, pp. 143 to 154, published 16/05/2024.

PDF Download PubMed Link Supplemental Information
Affiliations:

1Department of Mathematical Sciences, Chalmers University of Technology, University of Gothenburg, Sweden. 2Department of Biotechnology and Biosciences, University of Milano-Bicocca, Italy. 3Department of Biology and Biological Engineering, Department of Mathematical Sciences, Chalmers University of Technology, Sweden.

Keywords: 

yeast, nutrient signaling, Snf1/Mig1 network, nuclear localization, fluorescence recovery after photobleaching, nonlinear mixed effect model.

Corresponding Author(s):

Farida Tripodi, Department of Biotechnology and BiosciencesUniversity of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy; phone: +39 264483513; farida.tripodi1@unimib.it Marija Cvijovic, Department of Mathematical Sciences, Chalmers University of Technology and University of Gothenburg, SE-412 96 Gothenburg, Sweden; phone: +46 317725321; marija.cvijovic@chalmers.se

Conflict of interest statement:

None declared.

Please cite this article as:

Svenja Braam, Farida Tripodi, Linnea Österberg, Sebastian Persson, Niek Welkenhuysen, Paola Coccetti, Marija Cvijovic (2024). Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based a pproaches. Microbial Cell 11: 143-154. doi: 10.15698/mic2024.05.822

© 2024 Braam et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.

Abstract:

The AMPK/SNF1 pathway governs energy balance in eukaryotic cells, notably influencing glucose de-repression. In S. cerevisiae, Snf1 is phosphorylated and hence activated upon glucose depletion. This activation is required but is not sufficient for mediating glucose de-repression, indicating further glucose- dependent regulation mechanisms. Employing fluorescence recovery after photobleaching (FRAP) in conjunction with non-linear mixed effects modelling, we explore the spatial dynamics of Snf1 as well as the relationship between Snf1 phosphorylation and its target Mig1 controlled by hexose sugars. Our results suggest that inactivation of Snf1 modulates Mig1 localization and that the kinetic of Snf1 localization to the nucleus is modulated by the presence of non-fermentable carbon sources. Our data offer insight into the true complexity of regulation of this central signaling pathway in orchestrating cellular responses to fluctuating environmental cues. These insights not only expand our understanding of glucose homeostasis but also pave the way for further studies evaluating the importance of Snf1 localization in relation to its phosphorylation state and regulation of downstream targets.