Microreviews, Review

Where antibiotic resistance mutations meet quorum-sensing

Rok Krašovec1, Roman V. Belavkin2, John A.D. Aston3, Alastair Channon4, Elizabeth Aston4, Bharat M. Rash1, Manikandan Kadirvel5,6, Sarah Forbes6, and Christopher G. Knight1

This article comments on work published by Krašovec et al. (Nat Comm, 2014), which found that the modulation of de novo mutation to promote antibiotic resistance depends on the density of the bacterial population and cell-cell interactions (rather than, for instance, the level of stress).

Sphingolipids and mitochondrial function, lessons learned from yeast

Pieter Spincemaille1, Bruno P.A. Cammue1,2 and Karin Thevissen1

This article reviews recent research showing that Saccharomyces cerevisiae is an invaluable model to investigate sphingolipids as signaling molecules in modulating mitochondrial function, but can also be used as a tool to further enhance our current knowledge on sphingolipids and mitochondria in mammalian cells.

Genome evolution in yeast reveals connections between rare mutations in human cancer

Xinchen Teng1,2 and J. Marie Hardwick2

This article comments on work published by Teng et al. (Mol Cell, 2013), which, using the yeast knockout collections, provides hard evidence that single gene deletions/mutations in most non-essential genes can drive the selection for cancer-like mutations.

Cell-autonomous mechanisms of chronological aging in the yeast Saccharomyces cerevisiae

Anthony Arlia-Ciommo#, Anna Leonov#, Amanda Piano#, Veronika Svistkova# and Vladimir I. Titorenko

This article critically analyzes recent advances in the understanding of cell-autonomous mechanisms of chronological aging in the budding yeast Saccharomyces cerevisiae. It proposes a concept of a biomolecular network underlying the chronology of cellular aging in yeast, whichposits that such network progresses through a series of lifespan checkpoints.

Decoding the biosynthesis and function of diphthamide, an enigmatic modification of translation elongation factor 2 (EF2)

Raffael Schaffrath and Michael J. R. Stark

This article comments on work published by Uthman et al. (PLoS Genet, 2013), which suggests that Dph5 has a novel role as an EF2 inhibitor that affects cell growth when diphthamide synthesis is blocked or incomplete and shows that diphthamide promotes the accuracy of EF2 performance during translation.

Autophagy extends lifespan via vacuolar acidification

Christoph Ruckenstuhl1, Christine Netzberger1, Iryna Entfellner1, Didac Carmona-Gutierrez1, Thomas Kickenweiz1, Slaven Stekovic1, Christina Gleixner1, Christian Schmid1, Lisa Klug1, Ivan Hajnal1, Alice G. Sorgo1, Tobias Eisenberg1, Sabrina Büttner1, Guillermo Marin͂o2-4,  Rafal Koziel5, Christoph Magnes6, Frank Sinner6,7, Thomas R. Pieber6,7, Pidder Jansen-Dürr5, Kai-Uwe Fröhlich1, Guido Kroemer2,3,8-11, and Frank Madeo1

This article comments on work published by Ruckenstuhl et al. (PLoS Genet, 2014), which uses Saccharomyces cerevisiae to show that autophagy promotes lifespan extension upon MetR and requires the subsequent stimulation of vacuolar acidification, while it is epistatic to the equally autophagy-dependent anti-aging pathway triggered by TOR1 inhibition or deletion.

When less is more: hormesis against stress and disease

Andreas Zimmermann1, Maria A. Bauer1, Guido Kroemer2-5, Frank Madeo1 and Didac Carmona-Gutierrez1

This article condenses the conceptual and potentially therapeutic importance of hormesis by providing a short overview of current evidence in favor of the cytoprotective impact of hormesis, as well as of its underlying molecular mechanisms.

Morphed and moving: TNFα-driven motility promotes cell dissemination through MAP4K4-induced cytoskeleton remodeling

Min Ma1,2 and Martin Baumgartner1

This article comments on work published by Ma and Baumgartner (PLoS Patho, 2014), which investigated Theileria parasite control of host cell motile properties in the context of inflammatory signaling.

Hormesis: a fundamental concept in biology

Edward J. Calabrese

This article addresses the concept of hormetic dose response, which describes the limits to which integrative endpoints can be modulated (i.e., enhanced or diminished) by pharmaceutical, chemical and physical means.

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Inhibitors of glycosomal protein import provide new leads against trypanosomiasis

July 3, 2017

This article comments on work published by Dawidowski et al. (Science, 2017), which provides the grounds for further development of the glycosome inhibitors into clinical candidates and validates the parasite protein-protein interactions as drug targets.

Impact of the host on Toxoplasma stage differentiation

June 22, 2017

This review summarizes how Toxoplasma gondii transitions from an acute to a chronic infection in warm-blooded animals and humans through a developmental switch influenced by host cell physiology, which determines parasite persistence mainly in neural and muscular tissues.

Chlamydia and mitochondria – an unfragmented relationship

June 14, 2017

This article comments on work published by Chowdhury et al (J Cell Biol, 2017), which demonstrated that Chlamydia infection induces and requires an upregulation of the host miRNA, miR-30c-5p (miR-30c) to ameliorate infection induced stress on the host mitochondrial architecture and hinders induction of apoptosis.

Protein aggregation triggers a declining libido in elder yeasts that still have a lust for life

May 29, 2017

This article comments on work published by Schlissel et al (Science 2017), showing that aging in yeast does not lead to the expected loss of heterochromatin silencing due to Sir2 inactivity, but rather to reduced mating pheromone sensitivity caused by the aggregation of the RNA-binding protein Whi3, which can be reversed by eliminating Whi3's polyglutamine domain.

Post-transcriptional regulation of ribosome biogenesis in yeast

May 1, 2017

Microorganisms adapt to environmental changes by regulating their metabolism, and one key survival strategy is to decrease energy use during adverse conditions by halting ribosome production, with recent findings showing yeast can switch between pre-rRNA processing pathways in response to environmental shifts, adding complexity to ribosome biogenesis regulation.

Placeholder factors in ribosome biogenesis: please, pave my way

April 27, 2017

In ribosome synthesis, "placeholder" factors are crucial trans-acting elements that regulate the timing and assembly of ribosomal proteins, ensuring speed and accuracy in this intricate process by preventing premature interactions and guiding the proper formation of functional ribosomal subunits.

Insights from the redefinition of Helicobacter pylori lipopolysaccharide O-antigen and core-oligosaccharide domains

April 25, 2017

This article comments on work published by Li et al. (PloS Pathog, 2017), focusing on Helicobacter pylori infections. They are mostly asymptomatic but can lead to serious conditions, and H. pylori lipopolysaccharide (LPS) is crucial for colonization and persistence, making the study of its structure and biosynthesis pathway vital for understanding pathogenesis and developing treatments.

Evading plant immunity: feedback control of the T3SS in Pseudomonas syringae

March 17, 2017

This article comments on work published by Waite et al. (mBio, 2017), which indicates that a negative autogenous control mechanism, where the sigma factor HrpL represses its own expression, permits the plant pathogen Pseudomonas syringae to fine-tune its type III secretion system, potentially reducing the elicitation of plant immunity and enhancing its ability to cause disease.

Microbial flora, probiotics, Bacillus subtilis and the search for a long and healthy human longevity

March 16, 2017

This article comments on work published by Donato et al. (Nat Commun, 2017), which reveals that the probiotic Bacillus subtilis extends the lifespan of Caenorhabditis elegans via mechanisms including the formation of biofilms and the production of signaling molecules like NO and CSF, suggesting a potential pathway through insulin-like signaling that could impact human longevity and age-related diseases.

Chlamydia trachomatis’ struggle to keep its host alive

March 2, 2017

This article comments on work published by Sixt et al. (Cell Host Microbe, 2016), which analyzed a CpoS-deficient mutant yielding unique insights into the nature of cell-autonomous defense responses against Chlamydia.

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