Reviews

Regulation of anti-microbial autophagy by factors of the complement system

Christophe Viret1, Aurore Rozières1, Rémi Duclaux-Loras1, Gilles Boschetti1, Stéphane Nancey1 and
Mathias Faure1,2

This review explores emerging evidence that components of the complement system, beyond their traditional immune roles, modulate autophagy - particularly xenophagy - thereby influencing cell-autonomous antimicrobial responses during host-pathogen interactions.

Microfluidic techniques for separation of bacterial cells via taxis

Jyoti P. Gurung1, Murat Gel2,3 and Matthew A. B. Baker1,3

Microfluidic tools, ideal for studying microbial motility due to their control over laminar flows at microscopic scales, enable precise analysis of various taxis behaviors and have advanced applications in synthetic biology, directed evolution, and medical microbiology.

Influence of delivery and feeding mode in oral fungi colonization – a systematic review

Maria Joao Azevedo1,2,3,4, Maria de Lurdes Pereira1,5, Ricardo Araujo2,3,6, Carla Ramalho3,7,8, Egija Zaura4 and Benedita Sampaio-Maia1,2,3

A systematic review of oral fungal colonization in infants found that while breastfeeding did not significantly affect the oral mycobiome, vaginal delivery was associated with higher oral yeast colonization, particularly of Candida albicans.

The role of Lactobacillus species in the control of Candida via biotrophic interactions

Isabella Zangl1, Ildiko-Julia Pap2, Christoph Aspöck2 and Christoph Schüller1,3

Microbial communities, including Candida and Lactobacillus species, play a crucial role in human health, particularly in the context of mucosal infections, but our understanding of their interactions and effects is still incomplete due to the variability of species and isolates as well as the complexity of the human host.

Gut microbial metabolites in depression: understanding the biochemical mechanisms

Giorgia Caspani1, Sidney Kennedy2-5, Jane A. Foster6 and Jonathan Swann1

This article shows how the gut microbiota contributes to the pathophysiology of depression and examines the mechanisms by which microbially-derived molecules may influence depressive behavior, highlighting the potential of dietary interventions as novel therapeutic strategies.

The multiple functions of the numerous Chlamydia trachomatis secreted proteins: the tip of the iceberg

Joana N. Bugalhão1 and Luís Jaime Mota1

CThis article shows an in-depth review on the current knowledge and outstanding questions about secreted proteins from Chlamydia trachomatis, detailing their roles in host cell interaction and immune response evasion.

Diverse conditions support near-zero growth in yeast: Implications for the study of cell lifespan

Jordan Gulli1, Emily Cook1, Eugene Kroll1, Adam Rosebrock2,3, Amy Caudy2 and Frank Rosenzweig1

This review discusses alternative cultivation methods for baker's yeast to study its chronological lifespan, with the aim of better understanding the ageing of non-dividing cells and their potential implications for the lifespan of multicellular eukaryotes such as humans.

Integrins in disguise – mechanosensors in Saccharomyces cerevisiae as functional integrin analogues

Tarek Elhasi1 and Anders Blomberg1

This article shows that although yeast lack integrin-like proteins, they possess WSC- and MID-type mechanosensors that functionally resemble integrins in animal cells, playing a role in sensing external mechanical stimuli and activating the conserved PKC1-SLT1 cell wall integrity pathway, with potential implications for understanding mechanosensing in yeast biology.

Beyond cells – The virome in the human holobiont

Rodrigo García-López1,2,3, Vicente Pérez-Brocal1,2,3 and Andrés Moya1,2,3

This article provides an overview of viromics—viral metagenomics—and its evolution, highlighting the complexity and dynamic nature of viruses beyond their traditional view as pathogens. It acknowledges the technological advancements in molecular biology and sequencing that have allowed the field to grow, while also noting that viromic research still faces significant challenges and is not as developed as bacterial metagenomics. The review underscores the importance of viruses in various ecosystems, their role in shaping the genetic landscape, and their potential impact, and looks forward to future directions in viromic research.

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Why are essential genes essential? – The essentiality of Saccharomyces genes

July 25, 2015

Essential genes are defined as required for the survival of an organism or a cell. This article reviews and analyzes the levels of essentiality of the Saccharomyces cerevisiae genes and groups the genes into four categories: (1) Conditional essential: essential only under certain circumstances or growth conditions; (2) Essential: required for survival under optimal growth conditions; (3) Redundant essential: synthetic lethal due to redundant pathways or gene duplication; and (4) Absolute essential: the minimal genes required for maintaining a cellular life under a stress-free environment. The essential and non-essential functions of the essential genes are further analyzed.

Membrane depolarization-triggered responsive diversification leads to antibiotic tolerance

July 24, 2015

In this article, the authors discuss the article "Obg and membrane depolarization are part of a microbial bet-hedging strategy that leads to antibiotic tolerance", Verstraeten et al., Mol. Cell 2015 Jul 2; 59 (1): 9-21.

Evolutionary rewiring of bacterial regulatory networks

July 6, 2015

Bacteria have evolved complex regulatory networks that enable integration of multiple intracellular and extracellular signals to coordinate responses to environmental changes. However, our knowledge of how regulatory systems function and evolve is still relatively limited. There is often extensive homology between components of different networks, due to past cycles of gene duplication, divergence, and horizontal gene transfer, raising the possibility of cross-talk or redundancy. Consequently, evolutionary resilience is built into gene networks – homology between regulators can potentially allow rapid rescue of lost regulatory function across distant regions of the genome. This article discusses Taylor, et al. Science (2015), 347(6225), reporting mutations that facilitate cross-talk between pathways can contribute to gene network evolution, but which come with severe pleiotropic costs. Arising from this work are a number of questions surrounding how this phenomenon occurs.

Wanted Plasmodium falciparum, dead or alive

June 23, 2015

In this article, mechanisms of cell death in unicellular parasites are discussed, focussing on “programmed cell death” in Plasmodium.

Yeast as a tool to explore cathepsin D function

June 11, 2015

Cathepsin D has garnered increased attention in recent years, mainly since it has been associated with several human pathologies. This review summarizes how cathepsin D can have both anti- and pro-survival functions depending on its proteolytic activity, cellular context and stress stimulus.

Coordinate responses to alkaline pH stress in budding yeast

May 22, 2015

This review summarizes the modulation of a substantial number of signaling pathways whose participate in the alkaline response in yeast. These regulatory inputs involve not only the conserved Rim101/PacC pathway, but also the calcium-activated phosphatase calcineurin, the Wsc1-Pkc1-Slt2 MAP kinase, the Snf1 and PKA kinases and oxidative stress-response pathways.

Handcuffs for bacteria – NDP52 orchestrates xenophagy of intracellular Salmonella

May 21, 2015

This microreview discusses the article "Autophagy Receptor NDP52 Regulates Pathogen-Containing Autophagosome Maturation" by Verlhac et al. (2015), Cell Host Microbe.

Struggling for breath in Sherbrooke: 1st Symposium on “One mitochondrion, many diseases” in Sherbrooke, Québec, Canada, March 11th, 2015

May 20, 2015

This meeting report summarizes discussions during the "1st symposium on “One mitochondrion, many diseases," which took place in Sherbrooke in southern Québec in 2015.

Understanding grapevine-microbiome interactions: implications for viticulture industry

May 4, 2015

This microreview discusses the article "The soil microbiome influences grapevine-associated microbiota" by Zarraonaindia et al. (2015), MBio, which reports that the grapevine-associated microbiota depends on the soil microbiome.

Cytokinins beyond plants: synthesis by Mycobacterium tuberculosis

May 4, 2015

This microreview discusses "Proteasomal Control of Cytokinin Synthesis Protects Mycobacterium tuberculosis against Nitric Oxide" by Samanovic et al. (2015), Mol Cell.

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