Review, Reviews

Out with the old: Hsp90 finds amino acid residue more useful than co-chaperone protein

Abbey D. Zuehlke1 and Leonard Neckers1

This article comments on work published by Zuehlke et al (Nat Commun, 2017), which demonstrates that the function of one co-chaperone in yeast is replaced by posttranslational modification (PTM) of a single amino acid within Hsp90 in higher eukaryotes.

Having your cake and eating it – Staphylococcus aureus small colony variants can evolve faster growth rate without losing their antibiotic resistance

Gerrit Brandis1, Sha Cao1, Douglas L. Huseby1 and Diarmaid Hughes1

This article comments on work published by Cao et al. (mBio, 2027), which shows that Staphylococcus aureus can produce small colony variants (SCVs) that are challenging to detect and lead to persistent infections due to mutations affecting respiration and ATP production, with recent findings indicating various evolutionary paths for SCVs to increase growth rate while maintaining antibiotic resistance, suggesting greater adaptability and clinical challenge.

Integrative metabolomics as emerging tool to study autophagy regulation

Sarah Stryeck1, Ruth Birner-Gruenberger2, Tobias Madl1,*

This review summarizes the advancements in metabolomics, particularly using NMR spectroscopy and mass spectrometry, and its increasing role in biological research, offering insights into autophagy regulation with a focus on key metabolites, recent studies, and future prospects in elucidating complex regulatory mechanisms of autophagy and related diseases.

The interplay between transcription and mRNA degradation in Saccharomyces cerevisiae

Subhadeep Das1, Debasish Sarkar2 and Biswadip Das1

This review summarizes how the integration of mRNA synthesis and degradation, mediated by specialized promoters and "coordinators," shapes the cellular transcriptome and plays a significant role in regulating gene expression profiles in various biological processes and potentially enhances evolutionary rates.

Inhibitors of glycosomal protein import provide new leads against trypanosomiasis

Vishal C. Kalel1, Leonidas Emmanouilidis2,3, Maciej Dawidowski2,3,4, Wolfgang Schliebs1, Michael Sattler2,3, Grzegorz M. Popowicz2,3, Ralf Erdmann1

This article comments on work published by Dawidowski et al. (Science, 2017), which provides the grounds for further development of the glycosome inhibitors into clinical candidates and validates the parasite protein-protein interactions as drug targets.

Impact of the host on Toxoplasma stage differentiation

Carsten G.K. Lüder1 and Taibur Rahman1

This review summarizes how Toxoplasma gondii transitions from an acute to a chronic infection in warm-blooded animals and humans through a developmental switch influenced by host cell physiology, which determines parasite persistence mainly in neural and muscular tissues.

Chlamydia and mitochondria – an unfragmented relationship

Suvagata Roy Chowdhury1 and Thomas Rudel1

This article comments on work published by Chowdhury et al (J Cell Biol, 2017), which demonstrated that Chlamydia infection induces and requires an upregulation of the host miRNA, miR-30c-5p (miR-30c) to ameliorate infection induced stress on the host mitochondrial architecture and hinders induction of apoptosis.

Protein aggregation triggers a declining libido in elder yeasts that still have a lust for life

Fabrice Caudron

This article comments on work published by Schlissel et al (Science 2017), showing that aging in yeast does not lead to the expected loss of heterochromatin silencing due to Sir2 inactivity, but rather to reduced mating pheromone sensitivity caused by the aggregation of the RNA-binding protein Whi3, which can be reversed by eliminating Whi3's polyglutamine domain.

Post-transcriptional regulation of ribosome biogenesis in yeast

Isabelle C. Kos-Braun and Martin Koš

Microorganisms adapt to environmental changes by regulating their metabolism, and one key survival strategy is to decrease energy use during adverse conditions by halting ribosome production, with recent findings showing yeast can switch between pre-rRNA processing pathways in response to environmental shifts, adding complexity to ribosome biogenesis regulation.

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Extracellular vesicles: An emerging platform in gram-positive bacteria

October 5, 2020

Extracellular vesicles (EVs) are secreted by both pathogenic and non-pathogenic bacteria to transfer biomolecules and facilitate intercellular communication. While EV secretion in gram-negative bacteria is well understood, less is known about gram-positive bacteria. This review explores the role of EVs involved in bacterial competition, survival, immune evasion, and infection of gram-positive bacteria and compares them to gram-negative counterparts.

Structural insights into the architecture and assembly of eukaryotic flagella

September 21, 2020

Cilia and flagella are key structures in motility and signaling. This review highlights recent findings of cryo-EM studies that have mapped the structure of axonemal microtubules in Chlamydomonas reinhardtii, revealing over 30 associated proteins as well as recent researcht which focused on the trafficking complexes that transport components between the cell body and cilium.

Plant and fungal products that extend lifespan in Caenorhabditis elegans

July 9, 2020

Caenorhabditis elegans' lifespan is extended by plant and fungal extracts activating pathways like autophagy and mitochondrial biogenesis. Low to moderate concentrations promote longevity, while high doses are harmful. This review explores the health benefits of these substances in humans.

From the Uncharacterized Protein Family 0016 to the GDT1 family: Molecular insights into a newly-characterized family of cation secondary transporters

June 15, 2020

This review outlines how the formerly uncharacterized UPF0016 family, now known as the Gdt1 family, plays key roles in cation transport - especially Mn²⁺ - across species from bacteria to humans. These proteins are crucial for processes like glycosylation, photosynthesis, and calcium signaling, with functions linked to their localization in membranes such as the Golgi, chloroplast, and plasma membrane and by that highlighting their evolutionary conservation and physiological relevance, offering insights into their shared and distinct features across organisms.

Regulation of Cdc42 for polarized growth in budding yeast

May 19, 2020

This review highlights how studies in budding yeast have revealed a biphasic mechanism of Cdc42 activation that governs cell polarity establishment, with implications for understanding similar processes in mammalian cells and the role of Cdc42 in aging.

Yeast-based assays for the functional characterization of cancer-associated variants of human DNA repair genes

May 18, 2020

This article highlights how the genetic tractability and conserved DNA repair pathways of yeast make it a powerful system for functionally characterizing human cancer-associated variants in DNA repair genes, aiding in risk assessment and therapeutic decision-making.

Regulation of anti-microbial autophagy by factors of the complement system

March 19, 2020

This review explores emerging evidence that components of the complement system, beyond their traditional immune roles, modulate autophagy - particularly xenophagy - thereby influencing cell-autonomous antimicrobial responses during host-pathogen interactions.

Microfluidic techniques for separation of bacterial cells via taxis

January 15, 2020

Microfluidic tools, ideal for studying microbial motility due to their control over laminar flows at microscopic scales, enable precise analysis of various taxis behaviors and have advanced applications in synthetic biology, directed evolution, and medical microbiology.

Influence of delivery and feeding mode in oral fungi colonization – a systematic review

January 7, 2020

A systematic review of oral fungal colonization in infants found that while breastfeeding did not significantly affect the oral mycobiome, vaginal delivery was associated with higher oral yeast colonization, particularly of Candida albicans.

The role of Lactobacillus species in the control of Candida via biotrophic interactions

November 25, 2019

Microbial communities, including Candida and Lactobacillus species, play a crucial role in human health, particularly in the context of mucosal infections, but our understanding of their interactions and effects is still incomplete due to the variability of species and isolates as well as the complexity of the human host.

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