Research, Research Articles
Scp160 deletion suppresses TDP-43 aggregation and toxicity in Saccharomyces cerevisiae
Joan Castells-Ballestera and Natalia Shcherbik
Deletion of SCP160 or BFR1 reduces TDP-43 toxicity without lowering TDP-43 protein abundance and suppresses prominent TDP-43, Htt103Q, and VHL aggregation, while SCP160 deletion also shifts residual TDP-43 foci toward the ER.
The p53 DNA-Binding (Core) Domain can form a prion that seeds prion formation in full-length p53
Mathilde Kadouch1, Christelle Marchal1, Sei-Kyoung Park2, Sangeun Park2, Sabine Castano1, Susan W. Liebman2 and Christophe Cullin1
In yeast the p53 core-domain forms a self-propagating prion that inactivates a reporter and converts untagged full-length p53 to a prion. R175H in the tagged chimera shows 18x higher prionization, linking it to p53 inactivation in human cancers.
Sorbic acid triggers rapid formation of proteasome storage granules in Saccharomyces cerevisiae
Shoei Tanaka1, Mitsuki Imajo1, Mieko Hayashi2, Akira Matsuura2 and Shingo Izawa1
This study demonstrates that sorbic acid, a common food preservative, induces the fastest reported proteasome storage granule (PSG) formation in yeast within 30 min, highlighting differences from other PSG-inducing conditions like acetic acid stress.
A time-resolved high-throughput screening of fission yeast deletion mutants for oxidative stress resistance
Mohammadtaha Pirsalehi, Rowshan Ara Islam, Kristal Ng, Olga Xintarakou, Peter Harold Thorpe and Charalampos Rallis
By capturing dynamic trajectories rather than static outcomes, this study exposes hidden layers of growth under oxidative stress and identifies new genetic determinants of cellular resilience in fission yeast.
Clonal spread and environmental persistence of carbapenem-resistant high-risk Pseudomonas aeruginosa in critical-care units of a Chilean national referral center for burn and trauma patients (2022)
Camila Ibarra1, Gustavo Araya2, Alhejandra Alvarez2, Leonardo Enríquez2, Carolina Arellano2, Fabricio Alarcón1, Constanza Fernández1, Claudio Vargas1, Rodrigo Vera1 and Roberto M. Vidal2
This study highlight the circulation of high-risk P. aeruginosa clones in Chile and underscore the importance of molecular epidemiology in guiding infection control, optimizing antimicrobial therapy, and mitigating the clinical and economic burden of CRPA.
The transcriptome of dendritic cells redraws the boundaries between pathogenicity and commensalism in yeast
Lisa Rizzetto1, Irene Stefanini2, Stefano Nenciarini3, Monica Di Paola3, Samantha Riccadonna1, Ivo G. Gut4,5, Marta Gut4,5, Simon Heath4,5 and Duccio Cavalieri3
Our immune system meets many fungi, but reacts strongly to only a few. The comparison of key fungal species showed that responses differ mainly in timing and not just genes, highlighting the need to rethink what makes fungi harmful vs. harmless.
Yippee-like protein Moh1 links gene expression to metabolism and selective stress resistance in Saccharomyces cerevisiae
Çağla Ece Olgun1,a, Gizem Turan Duman1,a, Gizem Güpür1, Hamit İzgi1, Mariam Huda2, Demet Çetin3, Zekiye Suludere4, Fatma Küçük Baloğlu5, Ayşe Koca Çaydaşı2 and Mesut Muyan1
This study demonstrates that the conserved yeast protein Moh1 links metabolism, gene expression, and cell structure, thereby altering cell envelope permeability and leading to selective stress responses.
Fungistatic effect of sorbic acid on yeast cells via translational repression involving eIF2α phosphorylation and formation of Ded1- and eIF2B-granules
Haruka Yoshiyama1, Wataru Nomura2 and Shingo Izawa1
The findings of this study provide new insights into the physiological effects of sorbic acid on yeast cells, specifically regarding the regulation of translation-related factors.
Occlusal types shape oral microbiome stomatotypes and metabolic landscapes: A multi-omics perspective on host-microbe interaction
Yufeng Duan1,a, Zheng Liu2,3,a, Wenting Lu1,a, Ning Zhao1, Lingjun Yuan1, Zhenxia Li1, Ting Zhou1, Shengzhao Xiao1, Dian Jing1, Xiaowen Zheng1, Wentao Shi4, Chao Liu1,*, Haixia Lu5,*, Qiang Feng6,* and Bing Fang1,*
This study reveals how occlusal types shape oral microbiome “stomatotypes” and metabolic profiles in adolescents. It offers fresh insights that host anatomy drives microecology which may be associated with personalized oral health.
Fat storage-inducing transmembrane (FIT or FITM) proteins are related to lipid phosphatase/phosphotransferase enzymes
December 28, 2017
Fat storage-inducing transmembrane (FIT or FITM) proteins have been implicated in the partitioning of triacylglycerol to lipid droplets and the budding of lipid droplets from the ER. Saccharomyces cerevisiae has two FITM homologues and the presented results suggest that Scs3p and Yft2p as well as FITMs in general are lipid phosphatase/phosphotransferase (LPT) enzymes involved in an as yet unknown critical step in phospholipid metabolism.
A versatile plasmid system for reconstitution and analysis of mammalian ubiquitination cascades in yeast
December 5, 2017
In this article Avagliano Trezza et al. describe a versatile vector system that allows the reconstitution of specific ubiquitination cascades in the model eukaryote Saccharomyces cerevisae (baker’s yeast) that provides a versatile tool to study complex post-translational modifications in a cellular setting.
Alcohols enhance the rate of acetic acid diffusion in S. cerevisiae : biophysical mechanisms and implications for acetic acid tolerance
December 1, 2017
Microbial cell factories with the ability to maintain high productivity in the presence of weak organic acids, such as acetic acid, are required in many industrial processes. This study demonstrates that the rate of acetic acid diffusion can be strongly affected by compounds that partition into the cell membrane, and highlights the need for considering interaction effects between compounds in the design of microbial processes.
A genome-wide screen for FTY720-sensitive mutants reveals genes required for ROS homeostasis
November 27, 2017
Fingolimod hydrochloride (FTY720) is an immune modulator for multiple sclerosis that also induces cancer cell apoptosis through reactive oxygen species generation, with a new study using fission yeast uncovering a gene network related to ROS homeostasis as a possible mechanism of FTY720's toxicity.
The cytosolic glyoxalases of Plasmodium falciparum are dispensable during asexual blood-stage development
November 20, 2017
In this study the authors demonstrate that, PfGlo1 and PfcGlo2 are dispensable during asexual blood-stage development while the loss of PfcGlo2 may induce the formation of transmissible gametocytes. These combined data show that PfGlo1 and PfcGlo2 are most likely not suited as targets for selective drug development against the malaria parasite Plasmodium falciparum.
Aminoglycoside resistance profile and structural architecture of the aminoglycoside acetyltransferase AAC(6’)-Im
November 9, 2017
AAC(6')-Im, a monofunctional acetyltransferase, imparts increased resistance to certain aminoglycosides compared to its bifunctional homolog AAC(6')-Ie, with structural studies revealing differences in substrate binding that explain the discrepancies in their enzymatic activity and resistance profiles.
Cross-species complementation of bacterial- and eukaryotic-type cardiolipin synthases
November 3, 2017
This article shows that cardiolipin is crucial for cellular respiration and membrane integrity, with cardiolipin synthase enzymes like TbCLS in Trypanosoma brucei being potential drug targets due to their essential role in survival. The study demonstrates TbCLS's ability to restore cardiolipin production in yeast, highlighting the specificity and potential co-localization required for cardiolipin synthesis and remodeling, and underscoring the differences between eukaryotic and prokaryotic cardiolipin synthase mechanisms.
Identification of SUMO conjugation sites in the budding yeast proteome
October 2, 2017
The authors present a proteomic study that mapped SUMO acceptor lysines in budding yeast, identifying 257 potential conjugation sites, including both known and novel substrates, and providing a significant resource for future research into the functional implications of SUMOylation in yeast.
Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2
October 2, 2017
The authors descibe the role of the Hsp40 chaperone Ydj1 in Candida albicans, noting its localization to the cytosol and mitochondrial membrane, its necessity for stress responses and filamentation, and its involvement in a protein interaction network related to co-chaperones, filamentation regulators, and mitochondrial processing peptidases, with a particular focus on the impact of Ydj1 on mitochondrial morphology, function, and the import of precursor proteins.