Research – Page 2 – Microbial Cell

Research, Research Articles

Sir2 regulates selective autophagy in stationary-phase yeast cells

Ji-In Ryu^a, Juhye Jung^a, and Jeong-Yoon Kim

This study establishes Sir2 as a previously unrecognized regulator of selective autophagy during the stationary phase and highlight how cells dynamically control organelle degradation.

Luminal acetylation of microtubules is not essential for <i>Plasmodium berghei</i> and <i>Toxoplasma gondii</i> survival

Luminal acetylation of microtubules is not essential for Plasmodium berghei and Toxoplasma gondii survival

Thrishla Kumar^1,a, Katharina Röver^2,a, Johannes F. Stortz^3,a, Annika M. Binder^2,a, Benjamin Spreng², Madlen Konert², Markus Meissner¹, Friedrich Frischknecht^2,4 and Elena Jimenez-Ruiz^1,*

Acetylation of α-tubulin at lysine 40 is not essential for cytoskeletal stability in Plasmodium berghei or Toxoplasma gondii, suggesting redundancy and plasticity in microtubule regulation in these parasites.

The dual-site agonist for human M2 muscarinic receptors Iper-8-naphtalimide induces mitochondrial dysfunction in <i>Saccharomyces cerevisiae</i>

The dual-site agonist for human M2 muscarinic receptors Iper-8-naphtalimide induces mitochondrial dysfunction in Saccharomyces cerevisiae

Angela Cirigliano^1,a, Antonia Amelina^2,a, Elena Passarini², Alessandra Ricelli¹, Nicole Balasco¹, Mattia Mori³, Bruno Botta⁴, Maria Egle De Stefano^2,5, Claudio Papotto⁶, Claudia Guerriero², Ada Maria Tata^2,5 and Teresa Rinaldi^2,*

S. cerevisiae is a model to study human GPCRs. N-8-Iper, active against glioblastoma via M2 receptor, causes mitochondrial damage in yeast by binding Ste2, highlighting evolutionary conservation of GPCRs.

The core genetic drivers of chronological aging in yeast are universal regulators of longevity

Erika Cruz-Bonilla¹, Sergio E. Campos², Soledad Funes³, Cei Abreu-Goodger⁴ and Alexander DeLuna^1,2,^*

This study provides an integrated view of the core genetic landscape underlying aging in yeast, highlighting the value of the chronological lifespan paradigm for investigating conserved mechanisms of aging.

Organelle activity organized by the endoplasmic reticulum-mitochondria encounter structure –ERMES– is essential for <i>Podospora anserina</i> development

Organelle activity organized by the endoplasmic reticulum-mitochondria encounter structure –ERMES– is essential for Podospora anserina development

Melisa Álvarez-Sánchez¹, Matías Ramírez-Noguez¹, Beatriz Aguirre-López¹ and Leonardo Peraza-Reyes¹

This study shows that the regulation of the activity of multiple organelles by the ERMES (Endoplasmic Reticulum-Mitochondria Encounter Structure) tethering complex is essential for fungal growth and sexual development.

Role of the putative <i>sit1</i> gene in normal germination of spores and virulence of the <i>Mucor lusitanicus</i>

Role of the putative sit1 gene in normal germination of spores and virulence of the Mucor lusitanicus

Bernadett Vágó^1,2, Kitti Bauer^1,2, Naomi Varghese^1,2, Sándor Kiss-Vetráb^1,2, Sándor Kocsubé^1,2, Mónika Varga^1,2, András Szekeres^1,2, Csaba Vágvölgyi^1,2, Tamás Papp^1,2,3,# and Gábor Nagy^1,2,3,#

Mucormycosis is a life-threatening infection caused by certain members of the fungal order Mucorales, with increased incidence in recent years. Individuals with untreated diabetes mellitus, and patients treated with deferoxamine are particularly susceptible to this infection.

Tumor microenvironment signatures enhances lung adenocarcinoma prognosis prediction: Implication of intratumoral microbiota

Fei Zhao^1,#, Lei Wang^2,3,4,#, Dongjie Du⁵, Heaven Zhao^6,7, Geng Tian^6,7, Yufeng Li^2,3,8, Yankun Liu^2,8,9, Zhiwu Wang^2,3,10, Dasheng Liu¹¹, Jingwu Li^2,3,12, Lei Ji^6,7 and Hong Zhao¹

The interaction between intratumoral microbiome and the tumor microenvironment (TME) has furthered our understanding of tumor ecology. Yet, the implications of their interaction for lung cancer management remain unclear.

Persistence phenotype of adherent-invasive <i>Escherichia coli</i> in response to ciprofloxacin, revealing high-persistence strains

Persistence phenotype of adherent-invasive Escherichia coli in response to ciprofloxacin, revealing high-persistence strains

Valeria Pérez-Villalobos¹, Roberto Vidal², Marcela A. Hermoso^3,4 and Paula Bustamante¹

We investigated the roles of the resident antibiotic resistance plasmid, the stress response protein HtrA, and macrophage-induced persister formation. Our results revealed broad variability in persister cell formation among AIEC strains.

Knocking out histidine ammonia-lyase by using CRISPR-Cas9 abolishes histidine role in the bioenergetics and the life cycle of <i>Trypanosoma cruzi</i>

Knocking out histidine ammonia-lyase by using CRISPR-Cas9 abolishes histidine role in the bioenergetics and the life cycle of Trypanosoma cruzi

Janaína de Freitas Nascimento¹, María Julia Barisón¹, Gabriela Torres Montanaro¹, Letícia Marchese¹, Rodolpho Ornitz Oliveira Souza¹, Letícia Sophia Silva², Alessandra Aparecida Guarnieri² and Ariel Mariano Silber¹

Recent studies have highlighted the importance of this pathway in ATP production, redox balance, and the maintenance of cellular homeostasis in T. cruzi. In this work, we focus on the first step of the histidine degradation pathway, which is performed by the enzyme histidine ammonia lyase. Here we determined the kinetic and biochemical parameters of the T. cruzi histidine ammonia-lyase.

Previous Next

Sugar-induced cell death (SICD) in Saccharomyces cerevisiae: insights into nitrogen-mediated rescue and apoptotic cell death pathways

May 11, 2026

This study examined mechanisms through which yeast sugar-induced cell death can be prevented. High concentrations of glucose induced a catastrophic response that was only rescued by highly preferred nitrogen sources and by preventing nuclear localization of specific cell death proteins.

TOR-dependent regulation of the yeast homolog of the juvenile Batten Disease-associated gene CLN3

March 11, 2026

This study identifies conditions and genes that induce BTN1 expression in yeast. We show that BTN1 expression is regulated by translational control and by the mTOR1 pathway. An understanding of when and why BTN1 expression will aid in understanding the expression of CLN3, which may be helpful in the treatment of this devastating disease.

Metagenomic and microbiological analyses of historical manuscripts for bacterial community profiling and bacteria-related biodeterioration assessment

March 1, 2026

By documenting both culturable and non-culturable taxa, this work provides a foundational dataset for understanding bacterial contributions to manuscript stability and offers a methodological framework for future research on biodeterioration dynamics in Islamic and global documentary heritage.

Overcoming phagocytosis resistance of hypervirulent Klebsiella pneumoniae by directly targeting capsules

February 16, 2026

This study highlights a promising strategy for disarming hypervirulent K. pneumoniae by directly targeting its key virulence factors and provides novel insights into antibacterial therapeutic approaches against this clinically significant pathogen.

VapA/Scs2 sustains polarized growth in Aspergillus nidulans by maintaining AP-2-mediated apical endocytosis

February 5, 2026

To explore the functional significance of ER–PM contact sites in filamentous fungi, we identified and genetically characterized all Aspergillus nidulans proteins homologous to Snc2/VAP, Ist2, or tricalbins.

Genetic make-up and regulation of the L-lysine biosynthesis pathway in Vibrio natriegens

February 3, 2026

This study analysed the make-up and regulation of the biosynthetic pathway for L-lysine and related L-aspartate family amino acids (AFAAs) in Vibrio natriegens DSM759 to provide a comprehensive basis for future metabolic engineering endeavours aiming at developing this strain into an amino acid overproducer.

Regulation of extracellular vesicles for protein secretion in Aspergillus nidulans

January 28, 2026

This study reveals that Aspergillus nidulans boosts extracellular vesicle production when ER-trafficked enzymes are induced, uncovering how fungi remodel their secretome through vesicle-mediated secretion to adapt to changing environments and biofilm formation.