Microreviews, Review

Too much of a good thing: Overproduction of virulence factors impairs cryptococcal pathogenicity

Julia C. V. Reuwsaat1, Tamara L. Doering2, and Livia Kmetzsch1,3

This article comments on work published by Reuwsaat et al. (mBio, 2021), which identified the transcription factor Pdr802 as essential for Cryptococcus neoformans adaptation to and survival under host conditions both in vitro and in vivo.

Host-bacteria metabolic crosstalk drives S. aureus biofilm

Kira L. Tomlinson1 and Sebastián A. Riquelme1

This article comments on work published by Tomlinson et al. (Nat Comm, 2021), which demonstrates that Staphylococcus aureus induces the production of the immunoreglatory metabolite itaconate in airway immune cells by stimulating mitochondrial oxidant stress. Itaconate in turn inhibits S. aureus glycolysis and growth, and promoted carbon flux through bacterial metabolic pathways that support biofilm production.

Mechanisms underlying lactic acid tolerance and its influence on lactic acid production in Saccharomyces cerevisiae

Arne Peetermans1,2, María R. Foulquié-Moreno1,2 and Johan M. Thevelein1,2,3

This article reviews the manner in which Saccharomyces cerevisiae deals with the accumulation of lactic acid as a singular stress factor as well as in combination with other stresses. In addition, different methods to improve lactic acid tolerance in S. cerevisiae using targeted and non-targeted engineering methods are discussed.

When the pandemic opts for the lockdown: Secretion system evolution in the cholera bacterium

Francis J. Santoriello1,2 and Stefan Pukatzki1,2

This article comments on work published by Santoriello et al. (Nat Comm, 2020), which demonstrates that the T6SS island Auxiliary Cluster 3 (Aux3) is unique to pandemic strains of V. cholerae.

Biofilms by bacterial human pathogens: Clinical relevance – development, composition and regulation – therapeutical strategies

Adina Schulze1,#, Fabian Mitterer1,#, Joao P. Pombo1 and Stefan Schild1,2,3

This review focuses on bacterial biofilms formed by human pathogens, highlights their relevance for diverse diseases and discusses therapeutical intervention strategies targeting biofilms.

Maintaining phagosome integrity during fungal infection: do or die?

Mabel Yang1, Glenn F.W. Walpole1,2 and Johannes Westman1

This article refers to the paper "Lysosome Fusion Maintains Phagosome Integrity during Fungal Infection" by Westman et al. (Cell Host Microbe, 2020), which shows that macrophages respond to pathogen growth by expanding the phagosome membrane through a calcium-dependent mechanism involving lysosome insertion, maintaining membrane integrity and preventing rupture.

Milestones in Bacillus subtilis sporulation research

Eammon P. Riley1, Corinna Schwarz2, Alan I. Derman2 and Javier Lopez-Garrido2

In this review, the foundational discoveries that shaped the sporulation field are discussed, from its origins to the present day, tracing a chronology that spans more than one hundred eighty years.

A novel antibacterial strategy: histone and antimicrobial peptide synergy

Leora Duong1, Steven P. Gross2,3 and Albert Siryaporn1,3

This article refers to the study "Mammalian histones facilitate antimicrobial synergy by disrupting the bacterial proton gradient and chromosome organization" by Doolin et al. (Nat Comm, 2020) that shows that histones enhance the antimicrobial activity of peptides, disrupt bacterial membranes, and inhibit transcription, offering new insights into natural antimicrobial mechanisms.

Extracellular vesicles: An emerging platform in gram-positive bacteria

Swagata Bose1,#, Shifu Aggarwal1,#, Durg Vijai Singh1,2 and Narottam Acharya1

Extracellular vesicles (EVs) are secreted by both pathogenic and non-pathogenic bacteria to transfer biomolecules and facilitate intercellular communication. While EV secretion in gram-negative bacteria is well understood, less is known about gram-positive bacteria. This review explores the role of EVs involved in bacterial competition, survival, immune evasion, and infection of gram-positive bacteria and compares them to gram-negative counterparts.

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A comparative approach to decipher intestinal animal-microbe associations

October 31, 2018

In this article, the authors comment on the study "Chitin-based barrier immunity and its loss predated mucus-colonization by indigenous gut microbiota" by Nakashima et al. (Nat Commun, 2018) that used comparative analyses of chordates to investigate the development of animal-microbe associations, suggesting that microbial colonization of the mucus layer over mammalian gastrointestinal epithelium was established upon the loss of ancestral chitin-based barrier immunity, providing insights into the establishment of these associations in an evolutionary context.

Pathways of host cell exit by intracellular pathogens

October 18, 2018

This review provides an overview of the diverse host cell exit strategies employed by intracellular-living bacterial, fungal, and protozoan pathogens, highlighting the commonalities and system-specific variations of these strategies, and discussing potential microbial molecules involved in host cell exit as targets for future intervention approaches.

Conventional and emerging roles of the energy sensor Snf1/AMPK in Saccharomyces cerevisiae

September 29, 2018

This review consolidates current knowledge on the conventional and non-conventional functions of the effector kinase Snf1 in yeast, shedding light on its diverse roles in cellular physiology and energy homeostasis.

An unexpected benefit from E. coli: how enterobactin benefits host health

September 28, 2018

In this article, the authors comment on the study "Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase" by Qi et al. (Cell, 2018) that uncovered a surprising role for the Escherichia coli-produced siderophore enterobactin (Ent) in facilitating iron uptake by the host, marking a major shift in the understanding of its function and indicating potential new benefits from commensal bacteria in aiding the host's iron homeostasis.

Protective roles of ginseng against bacterial infection

September 19, 2018

This review highlights the antibacterial effects of ginseng against pathogenic bacterial infections, discussing its regulation of pathogenic factors and proposing the therapeutic potential of ginseng as a natural antibacterial drug to address antibiotic resistance and toxicity in the context of global public health challenges.

A Cinderella story: how the vacuolar proteases Pep4 and Prb1 do more than cleaning up the cell’s mass degradation processes

September 18, 2018

This review summarizes the expanded roles of the Saccharomyces cerevisiae vacuolar proteases Pep4 and Prb1 in non-vacuolar activities outside of autophagy, such as programmed cell death, protection from harmful protein forms, and gene expression regulation. The potential implications of these findings for fungal biology and drug target discovery, including insights for mammalian cell studies, are highlighted, emphasizing the need for a deeper understanding of these molecular processes.

The biosynthesis of pyoverdines

August 28, 2018

This review provides an overview of pyoverdine biosynthesis, emphasizing the distinctive fluorophore shared by various pyoverdines derived from ferribactins and the role of periplasmic processes in the maturation and modification of these siderophores, critical for the growth and colonization of hosts by fluorescent pseudomonads.

Toxin release mediated by the novel autolysin Cwp19 in Clostridium difficile

August 10, 2018

In this article, the authors comment on the study "Cwp19 is a novel lytic transglycosylase involved in stationary-phase autolysis resulting in toxin release in Clostridium difficile" by Wydau-Dematteis (MBio, 2018) that characterizes a novel peptidoglycan hydrolase, Cwp19, in Clostridioides difficile, highlighting its glucose-dependent mediation of toxins secretion and suggesting a potential role in the pathogenesis of this bacterium, contributing to the understanding of these enzymes in C. difficile and their implication in pathogenicity.

Escherichia coli hijack Caspr1 receptor to invade cerebral vascular and neuronal hosts

August 7, 2018

In this article, the authors comment on the study "Caspr1 is a host receptor for meningitis-causing Escherichia coli" by Zhao et al. (Nat Commun, 2ß18) that identified Caspr1 as a key host receptor for E. coli virulence factor IbeA, facilitating E. coli penetration through the blood-brain barrier (BBB). The research demonstrated that targeting the interaction between IbeA and Caspr1 could potentially neutralize E. coli virulence and prevented meningitis, shedding light on the mechanisms of bacterial invasion into brain endothelial cells and hippocampal neurons.

A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit

July 25, 2018

In this article, the authors comment on the study "Phosphoproteome dynamics during mitotic exit in budding yeast" by Touati (EMBO J, 2018) that described a time-resolved global phosphoproteome analysis during a cell cycle phase known as mitotic exit in budding yeast revealed the principles of phosphoregulation governing the ordered sequence of events such as spindle elongation, chromosome decondensation, and completion of cell division.

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