Review, Reviews

Too much of a good thing: Overproduction of virulence factors impairs cryptococcal pathogenicity

Julia C. V. Reuwsaat1, Tamara L. Doering2, and Livia Kmetzsch1,3

This article comments on work published by Reuwsaat et al. (mBio, 2021), which identified the transcription factor Pdr802 as essential for Cryptococcus neoformans adaptation to and survival under host conditions both in vitro and in vivo.

Host-bacteria metabolic crosstalk drives S. aureus biofilm

Kira L. Tomlinson1 and Sebastián A. Riquelme1

This article comments on work published by Tomlinson et al. (Nat Comm, 2021), which demonstrates that Staphylococcus aureus induces the production of the immunoreglatory metabolite itaconate in airway immune cells by stimulating mitochondrial oxidant stress. Itaconate in turn inhibits S. aureus glycolysis and growth, and promoted carbon flux through bacterial metabolic pathways that support biofilm production.

Mechanisms underlying lactic acid tolerance and its influence on lactic acid production in Saccharomyces cerevisiae

Arne Peetermans1,2, María R. Foulquié-Moreno1,2 and Johan M. Thevelein1,2,3

This article reviews the manner in which Saccharomyces cerevisiae deals with the accumulation of lactic acid as a singular stress factor as well as in combination with other stresses. In addition, different methods to improve lactic acid tolerance in S. cerevisiae using targeted and non-targeted engineering methods are discussed.

When the pandemic opts for the lockdown: Secretion system evolution in the cholera bacterium

Francis J. Santoriello1,2 and Stefan Pukatzki1,2

This article comments on work published by Santoriello et al. (Nat Comm, 2020), which demonstrates that the T6SS island Auxiliary Cluster 3 (Aux3) is unique to pandemic strains of V. cholerae.

Biofilms by bacterial human pathogens: Clinical relevance – development, composition and regulation – therapeutical strategies

Adina Schulze1,#, Fabian Mitterer1,#, Joao P. Pombo1 and Stefan Schild1,2,3

This review focuses on bacterial biofilms formed by human pathogens, highlights their relevance for diverse diseases and discusses therapeutical intervention strategies targeting biofilms.

Maintaining phagosome integrity during fungal infection: do or die?

Mabel Yang1, Glenn F.W. Walpole1,2 and Johannes Westman1

This article refers to the paper "Lysosome Fusion Maintains Phagosome Integrity during Fungal Infection" by Westman et al. (Cell Host Microbe, 2020), which shows that macrophages respond to pathogen growth by expanding the phagosome membrane through a calcium-dependent mechanism involving lysosome insertion, maintaining membrane integrity and preventing rupture.

Milestones in Bacillus subtilis sporulation research

Eammon P. Riley1, Corinna Schwarz2, Alan I. Derman2 and Javier Lopez-Garrido2

In this review, the foundational discoveries that shaped the sporulation field are discussed, from its origins to the present day, tracing a chronology that spans more than one hundred eighty years.

A novel antibacterial strategy: histone and antimicrobial peptide synergy

Leora Duong1, Steven P. Gross2,3 and Albert Siryaporn1,3

This article refers to the study "Mammalian histones facilitate antimicrobial synergy by disrupting the bacterial proton gradient and chromosome organization" by Doolin et al. (Nat Comm, 2020) that shows that histones enhance the antimicrobial activity of peptides, disrupt bacterial membranes, and inhibit transcription, offering new insights into natural antimicrobial mechanisms.

Extracellular vesicles: An emerging platform in gram-positive bacteria

Swagata Bose1,#, Shifu Aggarwal1,#, Durg Vijai Singh1,2 and Narottam Acharya1

Extracellular vesicles (EVs) are secreted by both pathogenic and non-pathogenic bacteria to transfer biomolecules and facilitate intercellular communication. While EV secretion in gram-negative bacteria is well understood, less is known about gram-positive bacteria. This review explores the role of EVs involved in bacterial competition, survival, immune evasion, and infection of gram-positive bacteria and compares them to gram-negative counterparts.

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The integrated stress response in budding yeast lifespan extension

October 24, 2017

This article summarizes how the budding yeast Saccharomyces cerevisiae has been instrumental in unraveling the molecular and cellular determinants of aging, and how the induction of cellular stress responses has been associated with experimental lifespan extension, thus underscoring the value of yeast as a model for developing potential aging therapies for humans.

Yeast for virus research

September 18, 2017

This article summarizes the use of budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe) in virus research, highlighting their advantages for studying viral replication, interaction with host cells, and fundamental cellular processes affected by viruses, while discussing their potential in analyzing small viral genomes and facilitating the discovery of antiviral drugs.

Macrophages as drivers of an opportunistic infection

September 13, 2017

This article comments on work published by Mesureur et al. (PloS Pathog, 2017), which shows that macrophages are essential for proliferation of B. cenocepacia in the host. This suggests a new paradigm for Bcc infections and urges the development of novel anti-infectious therapies to efficiently disarm these intrinsically antibiotic resistant facultative intracellular pathogens.

A yeast model for the mechanism of the Epstein-Barr virus immune evasion identifies a new therapeutic target to interfere with the virus stealthiness

August 31, 2017

This article comments on a publication by Lista et al. (Nature Communications, 2017) that uncovered the role of the host cell nucleolin (NCL) in EBNA1 self-limitation of expression via a direct interaction of this protein with G-quadruplexes (G4) formed in GAr-encoding sequence of EBNA1 mRNA.

Exacerbating and reversing lysosomal storage diseases: from yeast to humans

August 25, 2017

This article summarizes the use of yeast models in advancing our understanding of lysosomal storage diseases (LSDs), where they have been instrumental in researching LSD mechanisms, screening for therapeutic compounds, and exploring genetic and gene-environment interactions relevant to diseases like Batten disease, cystinosis, and Niemann-Pick type C disease, as well as their connection to broader health issues such as viral infections and obesity.

Live fast, die fast principle in a single cell of fission yeast

August 13, 2017

This article comments on a recent study (Nakaoka and Wakamoto, PLoS Biol, 2017), which developed a microfluidics-based platform to track multiple single cell lineages until death.

Out with the old: Hsp90 finds amino acid residue more useful than co-chaperone protein

August 1, 2017

This article comments on work published by Zuehlke et al (Nat Commun, 2017), which demonstrates that the function of one co-chaperone in yeast is replaced by posttranslational modification (PTM) of a single amino acid within Hsp90 in higher eukaryotes.

Having your cake and eating it – Staphylococcus aureus small colony variants can evolve faster growth rate without losing their antibiotic resistance

August 1, 2017

This article comments on work published by Cao et al. (mBio, 2027), which shows that Staphylococcus aureus can produce small colony variants (SCVs) that are challenging to detect and lead to persistent infections due to mutations affecting respiration and ATP production, with recent findings indicating various evolutionary paths for SCVs to increase growth rate while maintaining antibiotic resistance, suggesting greater adaptability and clinical challenge.

Integrative metabolomics as emerging tool to study autophagy regulation

July 14, 2017

This review summarizes the advancements in metabolomics, particularly using NMR spectroscopy and mass spectrometry, and its increasing role in biological research, offering insights into autophagy regulation with a focus on key metabolites, recent studies, and future prospects in elucidating complex regulatory mechanisms of autophagy and related diseases.

The interplay between transcription and mRNA degradation in Saccharomyces cerevisiae

July 3, 2017

This review summarizes how the integration of mRNA synthesis and degradation, mediated by specialized promoters and "coordinators," shapes the cellular transcriptome and plays a significant role in regulating gene expression profiles in various biological processes and potentially enhances evolutionary rates.

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