Review, Reviews

Mnemons: encoding memory by protein super-assembly

Fabrice Caudron and Yves Barral

This article comments on work published by Caudron and Barral (Cell, 2013), which proposes that polyQ- and polyN-based elements, termed mnemons, act as cellular memory devices to encode previous environmental conditions.

Fatal attraction in glycolysis: how Saccharomyces cerevisiae manages sudden transitions to high glucose

Johan H. van Heerden1,3,4, Meike T. Wortel1,3,4, Frank J. Bruggeman1,4, Joseph J. Heijnen2,3, Yves J.M. Bollen4,5, Robert Planqué6, Josephus Hulshof6, Tom G. O’Toole7, S. Aljoscha Wahl2,3 and Bas Teusink1,3,4

This article comments on work published by van Heerden et al. (Science, 2014), which demonstrates that the startup of glycolysis exhibits two dynamic fates: a proper, functional, steady state or the imbalanced state described above. Both states are stable, attracting states, and the probability distribution of initial states determines the fate of a yeast cell exposed to glucose.

Intersubunit communications within KaiC hexamers contribute the robust rhythmicity of the cyanobacterial circadian clock

Yohko Kitayama1, Taeko Nishiwaki-Ohkawa1,2 and Takao Kondo1

This article comments on work published by Kitayama et al. (Nat Comm, 2013), which suggests that intersubunit communication precisely synchronizes KaiC subunits to avoid dephasing, and contributes to the robustness of circadian rhythms in cyanobacteria.

Mitochondrial protein import under kinase surveillance

Magdalena Opalińska1 and Chris Meisinger1,2

This article summarizes recent discoveries in the yeast Saccharomyces cerevisiae model system that point towards a vital role of reversible phosphorylation in regulation of mitochondrial protein import.

Building a flagellum in biological outer space

Lewis D. B. Evans, Colin Hughes and Gillian M. Fraser

This article comments on work published by Evans et al. (Nature, 2013), which presents a simple and elegant transit mechanism in which growth is powered by the subunits themselves as they link head-to-tail in a chain that is pulled through the length of the growing structure to the tip. This new mechanism answers an old question and may have resonance in other assembly processes.

A novel mechanism involved in the coupling of mitochondrial biogenesis to oxidative phosphorylation

Jelena Ostojić1, Jean-Paul di Rago2,3, Geneviève Dujardin1,*

This article comments on a study by Ostojić et al. (Cell Metabolism, 2013), which has uncovered a regulatory loop by which the biogenesis of a major enzyme of the OXPHOS pathway, the respiratory complex III, is coupled to the energy producing activity of the mitochondria.

Stalling autophagy: a new function for Listeria phospholipases

Ivan Tattoli1,2, Matthew T. Sorbara2, Dana J. Philpott2 and Stephen E. Girardin1,*

This article comments on a study biy Tattoli et al. (EMBO J, 2013), which demonstrated that Listeria PI-PLC and PC-PLC contribute to the bacterial escape from autophagy through a mechanism that involves direct inhibition of the autophagic flux in the infected cells

Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography

Wei Dai1, Michael F. Schmid1, Jonathan A. King2, Wah Chiu1,*

Thiswork comments on a study by Dai et al. (Nature 2013) that illustrates that electron cryo-tomography is an approach whereby one can capture directly structural snapshots of transient phage assembly intermediates during maturation process. Such analysis can be generalizable not only to human viruses in human cells but also various molecular machines undergoing biological processes.

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The integrated stress response in budding yeast lifespan extension

October 24, 2017

This article summarizes how the budding yeast Saccharomyces cerevisiae has been instrumental in unraveling the molecular and cellular determinants of aging, and how the induction of cellular stress responses has been associated with experimental lifespan extension, thus underscoring the value of yeast as a model for developing potential aging therapies for humans.

Yeast for virus research

September 18, 2017

This article summarizes the use of budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe) in virus research, highlighting their advantages for studying viral replication, interaction with host cells, and fundamental cellular processes affected by viruses, while discussing their potential in analyzing small viral genomes and facilitating the discovery of antiviral drugs.

Macrophages as drivers of an opportunistic infection

September 13, 2017

This article comments on work published by Mesureur et al. (PloS Pathog, 2017), which shows that macrophages are essential for proliferation of B. cenocepacia in the host. This suggests a new paradigm for Bcc infections and urges the development of novel anti-infectious therapies to efficiently disarm these intrinsically antibiotic resistant facultative intracellular pathogens.

A yeast model for the mechanism of the Epstein-Barr virus immune evasion identifies a new therapeutic target to interfere with the virus stealthiness

August 31, 2017

This article comments on a publication by Lista et al. (Nature Communications, 2017) that uncovered the role of the host cell nucleolin (NCL) in EBNA1 self-limitation of expression via a direct interaction of this protein with G-quadruplexes (G4) formed in GAr-encoding sequence of EBNA1 mRNA.

Exacerbating and reversing lysosomal storage diseases: from yeast to humans

August 25, 2017

This article summarizes the use of yeast models in advancing our understanding of lysosomal storage diseases (LSDs), where they have been instrumental in researching LSD mechanisms, screening for therapeutic compounds, and exploring genetic and gene-environment interactions relevant to diseases like Batten disease, cystinosis, and Niemann-Pick type C disease, as well as their connection to broader health issues such as viral infections and obesity.

Live fast, die fast principle in a single cell of fission yeast

August 13, 2017

This article comments on a recent study (Nakaoka and Wakamoto, PLoS Biol, 2017), which developed a microfluidics-based platform to track multiple single cell lineages until death.

Out with the old: Hsp90 finds amino acid residue more useful than co-chaperone protein

August 1, 2017

This article comments on work published by Zuehlke et al (Nat Commun, 2017), which demonstrates that the function of one co-chaperone in yeast is replaced by posttranslational modification (PTM) of a single amino acid within Hsp90 in higher eukaryotes.

Having your cake and eating it – Staphylococcus aureus small colony variants can evolve faster growth rate without losing their antibiotic resistance

August 1, 2017

This article comments on work published by Cao et al. (mBio, 2027), which shows that Staphylococcus aureus can produce small colony variants (SCVs) that are challenging to detect and lead to persistent infections due to mutations affecting respiration and ATP production, with recent findings indicating various evolutionary paths for SCVs to increase growth rate while maintaining antibiotic resistance, suggesting greater adaptability and clinical challenge.

Integrative metabolomics as emerging tool to study autophagy regulation

July 14, 2017

This review summarizes the advancements in metabolomics, particularly using NMR spectroscopy and mass spectrometry, and its increasing role in biological research, offering insights into autophagy regulation with a focus on key metabolites, recent studies, and future prospects in elucidating complex regulatory mechanisms of autophagy and related diseases.

The interplay between transcription and mRNA degradation in Saccharomyces cerevisiae

July 3, 2017

This review summarizes how the integration of mRNA synthesis and degradation, mediated by specialized promoters and "coordinators," shapes the cellular transcriptome and plays a significant role in regulating gene expression profiles in various biological processes and potentially enhances evolutionary rates.

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