Review, Reviews

The molecular and cellular action properties of artemisinins: what has yeast told us?

Chen Sun and Bing Zhou

Artemisinin (ART) or Qinghaosu is a natural compound possessing superior anti-malarial activity. Although intensive studies have been done in the medicinal chemistry field to understand the structure-effect relationship, the biological actions of artemisinin are poorly understood and controversial. This review summarizes what we have learned from yeast about the basic biological properties of ARTs, as well as some key unanswered questions.

Metabolic network structure and function in bacteria goes beyond conserved enzyme components

Jannell V. Bazurto# and Diana M. Downs

This article comments on work published by Bazurto et al. (MBio, 2016), which demonstrated that conservation of metabolic components was not sufficient to predict network structure and function Escherichia coli.

Chemical proteomics approach reveals the direct targets and the heme-dependent activation mechanism of artemisinin in Plasmodium falciparum using an activity-based artemisinin probe

Jigang Wang1,2,# and Qingsong Lin2

This article comments on work published by Wang et al. (Nat Commun, 2014), which provides insights into the mode-of-action of artemisinin and its specificity against malaria parasites.

Translational repression in malaria sporozoites

Oliver Turque1, Tiffany Tsao1, Thomas Li1 and Min Zhang1,2

This article comments on work published by Zhang et al. (PLoS Pathog, 2016), which summarizea recent advances in the translational repression of gene expression in the malaria sporozoite.

Chromatin binding and silencing: Two roles of the same protein Lem2

Ramón Ramos Barrales and Sigurd Braun

This article comments on work published by Barrales et al. (Genes Dev, 2016), which identifies the nuclear envelope protein Lem2, a homolog of metazoan lamin-associated proteins (LAPs), as a relevant factor for heterochromatin silencing and perinuclear localization in the fission yeast Schizosaccharomyces pombe.

When and where? Pathogenic Escherichia coli differentially sense host D-serine using a universal transporter system to monitor their environment

James P. R. Connolly and Andrew J. Roe

This article comments on work published by Connolly et al. (PLoS Pathog, 2016), which describes the discovery of a functional and previously uncharacterized D-serine uptake system in E. coli.

Signaling pathways and posttranslational modifications of tau in Alzheimer’s disease: the humanization of yeast cells

Jürgen J. Heinisch1 and Roland Brandt2

In the past decade, yeast have been frequently employed to study the molecular mechanisms of human neurodegenerative diseases, generally by means of heterologous expression of genes encoding the relevant hallmark proteins. Substantial posttranslational modifications of many of these proteins are required for the development and progression of potentially disease relevant changes. We give an overview on common modifications as they occur in tau during AD and discuss potential approaches to humanize yeast in order to create modification patterns resembling the situation in mammalian cells.

The bacterial cell cycle checkpoint protein Obg and its role in programmed cell death

Liselot Dewachter1, Natalie Verstraeten1, Maarten Fauvart1,2 and Jan Michiels1

This article comments on work published by Dewachter et al. (mBio, 2015), which identified a programmed cell death mechanism in Escherichia coli that is triggered by a mutant isoform of the essential GTPase ObgE.

Bactericidal antibiotics induce programmed metabolic toxicity

Aislinn D. Rowan, Damien J. Cabral and Peter Belenky

This article comments on work published by Lobritz et al. (PNAS, 2015), which demonstrates that bactericidal antibiotics induce metabolic perturbations that are linked to and required for bactericidal antibiotic toxicity.

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Gut microbial metabolites in depression: understanding the biochemical mechanisms

September 27, 2019

This article shows how the gut microbiota contributes to the pathophysiology of depression and examines the mechanisms by which microbially-derived molecules may influence depressive behavior, highlighting the potential of dietary interventions as novel therapeutic strategies.

The multiple functions of the numerous Chlamydia trachomatis secreted proteins: the tip of the iceberg

August 21, 2019

CThis article shows an in-depth review on the current knowledge and outstanding questions about secreted proteins from Chlamydia trachomatis, detailing their roles in host cell interaction and immune response evasion.

Diverse conditions support near-zero growth in yeast: Implications for the study of cell lifespan

August 20, 2019

This review discusses alternative cultivation methods for baker's yeast to study its chronological lifespan, with the aim of better understanding the ageing of non-dividing cells and their potential implications for the lifespan of multicellular eukaryotes such as humans.

Integrins in disguise – mechanosensors in Saccharomyces cerevisiae as functional integrin analogues

July 15, 2019

This article shows that although yeast lack integrin-like proteins, they possess WSC- and MID-type mechanosensors that functionally resemble integrins in animal cells, playing a role in sensing external mechanical stimuli and activating the conserved PKC1-SLT1 cell wall integrity pathway, with potential implications for understanding mechanosensing in yeast biology.

Beyond cells – The virome in the human holobiont

July 1, 2019

This article provides an overview of viromics—viral metagenomics—and its evolution, highlighting the complexity and dynamic nature of viruses beyond their traditional view as pathogens. It acknowledges the technological advancements in molecular biology and sequencing that have allowed the field to grow, while also noting that viromic research still faces significant challenges and is not as developed as bacterial metagenomics. The review underscores the importance of viruses in various ecosystems, their role in shaping the genetic landscape, and their potential impact, and looks forward to future directions in viromic research.

Laundry and textile hygiene in healthcare and beyond

July 1, 2019

This article shows that while institutional laundering is regulated to ensure hygiene, the trend towards energy-efficient washing at lower temperatures raises concerns about the antimicrobial efficacy of domestic laundering, with a focus on addressing microbial contamination in both clinical and home settings.

Yeast AP-1 like transcription factors (Yap) and stress response: a current overview

May 28, 2019

This review summarizes current understanding of the eight Yap transcription factors in Saccharomyces cerevisiae, detailing their activation by specific stress conditions and discussing their function and evolution across various fungal species.

The influence of the microbiota on immune development, chronic inflammation, and cancer in the context of aging

May 13, 2019

This article shows that the microbiota is crucial for immune system development and that its relationship with the immune system during aging and the pathogenesis of age-related diseases, including cancer, needs further research to inform disease treatment and prevention.

Ser/Thr protein phosphatases in fungi: structure, regulation and function

April 24, 2019

In this work we present the members of this family in S. cerevisiae and other fungal species, and review the most recent findings concerning their regulation and the roles they play in the most diverse aspects of cell biology.

Forty-five-year evolution of probiotic therapy

April 2, 2019

The field of probiotics has greatly expanded over the past 45 years, driven by the need for safer alternatives to drugs, interest in natural microbial products, and clinical proof of effectiveness, with scientific formulations increasingly defining the market and promising applications for various health areas expected in the future.

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