Quantifying yeast lipidomics by high-performance thin-layer chromatography (HPTLC) and comparison to mass spectrometry-based shotgun lipidomics
Authors:Thorsten Meyer1, Oskar Knittelfelder2, Martin Smolnig1 and Patrick Rockenfeller1
doi: 10.15698/mic2024.02.815
Volume 11, pp. 57 to 68, published 21/02/2024.
1 Chair of Biochemistry and Molecular Medicine, Center for Biomedical Education and Research (ZBAF), University of Witten/Herdecke (UW/H), Stockumer Str. 10, 58453 Witten, Germany.
2 Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Keywords:
lipidomics, lipid metabolism, lipid analysis, HPTLC, thin-layer chromatography.
Corresponding Author(s):
Conflict of interest statement:
The authors declare no conflict of interest.
Please cite this article as:
Thorsten Meyer, Oskar Knittelfelder, Martin Smolnig and Patrick Rockenfeller (2024). Quantifying yeast lipidomics by high-performance thin-layer chromatography (HPTLC) and comparison to mass spectrometry-based shotgun lipidomics. Microbial Cell 11: 57-68. doi: 10.15698/mic2024.02.815
© 2024 Meyer et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
Abstract:
Lipidomic analysis in diverse biological settings has become a frequent tool to increase our understanding of the processes of life. Cellular lipids play important roles not only as being the main components of cellular membranes, but also in the regulation of cell homeostasis as lipid signaling molecules. Yeast has been harnessed for biomedical research based on its good conservation of genetics and fundamental cell organisation principles and molecular pathways. Further application in so-called humanised yeast models have been developed which take advantage of yeast as providing the basics of a living cell with full control over heterologous expression. Here we present evidence that high-performance thin-layer chromatography (HPTLC) represents an effective alternative to replace cost intensive mass spectrometry-based lipidomic analyses. We provide statistical comparison of identical samples by both methods, which support the use of HPTLC for quantitative analysis of the main yeast lipid classes.