Editorial

Groupthink: chromosomal clustering during transcriptional memory

Kevin A. Morano

In this article, the authors comment on the study "NO1 transcriptional memory leads to DNA zip code-dependent interchromosomal clustering." by Brickner et al. (Microbial Cell, 2015), discussing the importance and molecular mechanisms of chromosomal clustering during transcriptional memory.

Starting with a degron: N-terminal formyl-methionine of nascent bacterial proteins contributes to their proteolytic control

R. Jürgen Dohmen

In this article, the author comments on the study "Formyl-methionine as a degradation signal at the N-termini of bacterial proteins." by Piatkov et al. (Microbial Cell, 2015), discussing a novel N-terminal degradation signal (N-degron) that targets nascent proteins for degradation in Escherichia coli by a new branch of the bacterial N-end rule pathway, termed the fMet/N-end rule pathway

Feelin’ it: Differential oxidative stress sensing mediated by Cyclin C

W. Scott Moye-Rowley

Microbial cells that live exposed directly to their environmental milieu are faced with the challenge of adapting to the dynamic stress conditions that will inevitably be encountered. These stress conditions may vary over wide ranges and the most efficient responses would be tuned to produce a proportional buffering change. A mild stress would most efficiently be dealt with by a mild metabolic reprogramming that would prevent serious damage. A more severe environmental challenge would demand a more dramatic cellular compensatory response.

Subverting lysosomal function in Trypanosoma brucei

Sam Alsford

This article discusses Koh et al. (2015) "The lysosomotropic drug LeuLeu-OMe induces lysosome disruption and autophagy-independent cell death in Trypanosoma brucei (Microbial Cell 2(8): 288-298).

Entamoeba histolytica – tumor necrosis factor: a fatal attraction

Serge Ankri

This article comments on the study "In Entamoeba histolytica, a BspA family protein is required for chemotaxis toward tumour necrosis factor" by Silvestre et al. (Microbial Cell, 2015).

A safety catch for ornithine decarboxylase degradation

Christof Taxis

Feedback inhibition is a common mechanism to adjust the activity of an enzyme in accordance with the abundance of a product. This article comments on the study "Polyamines directly promote antizyme-mediated degradation of ornithine decarboxylase by the proteasome" by Beenukumar et al. (2015), Microbial Cell.

Metabolites in aging and autophagy

Sabrina Schroeder1,#, Andreas Zimmermann1,#, Didac Carmona-Gutierrez1, Tobias Eisenberg1, Christoph Ruckenstuhl1, Aleksandra Andryushkova1, Tobias Pendl1, Alexandra Harger1,2 and Frank Madeo1

This article analyzes the implications of specific metabolites in aging and autophagy with special emphasis on polyamine metabolism.

One cell, one love: a journal for microbial research

Didac Carmona-Gutierrez1, Guido Kroemer2-6 and Frank Madeo1

In this inaugural article of Microbial Cell, we highlight the importance of microbial research in general and the journal's intention to serve as a publishing forum that supports and enfolds the scientific diversity in this area as it provides a unique, high-quality and universally accessible source of information and inspiration.

Using microbes as a key tool to unravel the mechanism of autophagy and the functions of the ATG proteins

December 30, 2016

Microbes have served to discover and characterize unconventional functions of the ATG proteins, which are uncoupled from their role in autophagy. In our recent study, we have taken advantage of viruses as a screening tool to determine the extent of the unconventional functions of the ATG proteome and characterize one of them.

Autophagy: one more Nobel Prize for yeast

December 5, 2016

The recent announcement of the 2016 Nobel Prize in Physiology or Medicine, awarded to Yoshinori Ohsumifor the discoveries of mechanisms governing autophagy, underscores the importance of intracellular degradation and recycling. Here we provide a quick historical overview that mirrors both the importance of autophagy as a conserved and essential process for cellular life and death as well as the crucial role of yeast in its mechanistic characterization.

Physiology, phylogeny, and LUCA

November 25, 2016

Genomes record their own history. But if we want to look all the way back to life's beginnings some 4 billion years ago, the record of microbial evolution that is preserved in prokaryotic genomes is not easy to read. The classical approach has been to look for genes that are universally distributed. Another approach is to make all trees for all genes, and sift out the trees where signals have been overwritten by lateral gene transfer. What is left ought to be ancient. If we do that, what do we find?

The curious case of vanishing mitochondria

September 30, 2016

Due to their involvement in the energy metabolism, mitochondria are essential for most eukaryotic cells. Microbial eukaryotes living in low oxygen environments possess reduced forms of mitochondria, namely mitochondrion-related organelles (MROs). Recently, the first microbial eukaryote with neither mitochondrion nor MRO was characterized – Monocercomonoides sp. The discovery of such bona fide amitochondriate eukaryote broadens our knowledge about the diversity and plasticity of eukaryotic cells and provides a substantial contribution to our understanding of eukaryotic cell evolution.

Accumulation of metabolic side products might favor the production of ethanol in Pho13 knockout strains

September 23, 2016

This article comments on work published by Collard et al. (Nat Chem Biol, 2016), which describes the discovery of a striking example illustrating the metabolite repair concept.

Sexually transmitted infections: old foes on the rise

September 4, 2016

Sexually transmitted infections (STIs) are commonly spread via sexual contact. It is estimated that one million STIs are acquired every day worldwide. Besides their impact on sexual, reproductive and neonatal health, they can cause disastrous and life-threatening complications if left untreated. In addition to this personal burden, STIs also represent a socioeconomic problem, deriving in treatment costs of tremendous proportions. Despite a substantial progress in diagnosis, treatment and prevention, the incidence of many common STIs is increasing, and STIs continue to represent a global public health problem and a major cause for morbidity and mortality. With this Special Issue, Microbial Cell provides an in-depth overview of the eight major STIs, covering all relevant features of each infection.

Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation.

August 1, 2016

Diploid budding yeast (Saccharomyces cerevisiae) can adopt one of several alternative differentiation fates in response to nutrient limitation, and each of these fates provides distinct biological functions. When different strain backgrounds are taken into account, these various fates occur in response to similar environmental cues, are regulated by the same signal transduction pathways, and share many of the same master regulators. I propose that the relationships between fate choice, environmental cues and signaling pathways are not Boolean, but involve graded levels of signals, pathway activation and master-regulator activity.

Phosphatidylthreonine: An exclusive phospholipid regulating calcium homeostasis and virulence in a parasitic protist

May 1, 2016

This article comments on work published by Kuchipudi et al. (Microbial Cell, 2016), which describes the role of phohsphatidylthreonine in the regulation of calcium homeostasis and virulence in the protozoan parasite Toxoplasma gondii.

Non-genetic impact factors on chronological lifespan and stress resistance of baker’s yeast

April 13, 2016

This article comments on work published by Bisschops et al. (Microbial Cell, 2015), which illustrates how important the choice of the experimental setup is and how culture conditions influcence cellular aging and survival in biotechnological processes.

What’s old is new again: yeast mutant screens in the era of pooled segregant analysis by genome sequencing

April 4, 2016

This article comments on work published by Den Abt et al. (Microbial Cell, 2016), which identified genes involved in ethyl acetate formation in a yeast mutant screen based on a new approach combining repeated rounds of chemical mutagenesis and pooled segregant analysis by whole genome sequencing.