News and thoughts

Fancy a gene? A surprisingly complex evolutionary history of peroxiredoxins.

Alena Zíková1,2, Miroslav Oborník1,2,3 and Julius Lukeš1,2,4

In this comment, the authors discuss the article "Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites" (Djuika et al., Microbial Cell 2015).

The emerging role of complex modifications of tRNALysUUU in signaling pathways

Patrick C. Thiaville1,2,3,4 and Valérie de Crécy-Lagard2,4

This comment discusses the article "Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling" by Scheidt et al (Microbial Cell, 2014).

Metabolic pathways further increase the complexity of cell size control in budding yeast

Jorrit M. Enserink

This article comments on work published by Soma et al. (Microbial Cell, 2014), which teased apart the effect of metabolism and growth rate on setting of critical cell size in Saccharomyces cerevisiae.

Only functional localization is faithful localization

Roland Lill1,2,3

This article comments on work published by Peleh et al. (Microbial Cell 2014), which analyzes the localization of Dre2 in Saccharomyces cerevisiae.

What’s the role of autophagy in trypanosomes?

Katherine Figarella1 and Néstor L. Uzcátegui1,2

This article comments on Proto et al. (Microbial Cell, 2014), who report first insights into the molecular mechanism of autophagy in African trypanosomes by generating reporter bloodstream form cell lines.

Flagellated bacterial porter for in situ tumor vaccine

August 24, 2022

Cancer immunotherapy, which use the own immune system to attack tumors, are increasingly popular treatments. But, due to the tumor immunosuppressive microenvironment, the antigen presentation in the tumor is limited. Recently, a growing number of people use bacteria to stimulate the body's immunity for tumor treatment due to bacteria themselves have a variety of elements that activate Toll-like receptors. Here, we discuss the use of motility of flagellate bacteria to transport antigens to the tumor periphery to activate peritumoral dendritic cells to enhance the effect of in situ tumor vaccines.

Yeast goes viral: probing SARS-CoV-2 biology using S. cerevisiae

March 21, 2022

The budding yeast Saccharomyces cerevisiae has long been an outstanding platform for understanding the biology of eukaryotic cells. Robust genetics, cell biology, molecular biology, and biochemistry complement deep and detailed genome annotation, a multitude of genome-scale strain collections for functional genomics, and substantial gene conservation with Metazoa to comprise a powerful model for modern biological research. Recently, the yeast model has demonstrated its utility in a perhaps unexpected area, that of eukaryotic virology. Here we discuss three innovative applications of the yeast model system to reveal functions and investigate variants of proteins encoded by the SARS-CoV-2 virus.

Urm1, not quite a ubiquitin-like modifier?

September 21, 2021

This article comments on work published by Brachmann et al. (Redox Biol, 2020), which studied urmylation of the yeast 2-Cys peroxiredoxin Ahp1, uncovering that promiscuous lysine target sites and specific redox requirements determine the Urm1 acceptor activity of the peroxiredoxin.

Lichens – growing greenhouses en miniature

March 1, 2021

This commentary article provides an overview on different aspects of lichen biology and the remarkable symbiotic association between fungi and algae.

Raman-based sorting of microbial cells to link functions to their genes

February 10, 2020

In this article, the authors comment on the study "An automated Raman-based platform for the sorting of live cells by functional properties" by Lee et al. (Nat Microbiol, 2019), which presents a high-throughput optofluidic platform that integrates Raman microspectroscopy and microfluidics to accurately link microbial phenotypes to genotypes within complex communities, enabling efficient functional sorting and analysis of microbiome members.

Viral attenuation by Endonuclease G during yeast gametogenesis: insights into ancestral roles of programmed cell death?

December 17, 2019

This article relates to the study "Meiotic viral attenuation through an ancestral apoptotic pathway" by Gao et al. (Proc Natl Acad Sci, 2019), which shows that programmed cell death may have evolved as a viral defence mechanism, as demonstrated by yeast studies showing that the mitochondrial nuclease Nuc1 translocates to the cytosol during meiosis to attenuate dsRNA viruses, linking viral control to meiotic cell death processes.

Sulfur dioxide resistance in Saccharomyces cerevisiae: beyond SSU1

November 21, 2019

This article discusses the importance of understanding sulfite resistance in Saccharomyces cerevisiae due to its use in winemaking and the potential role of the transcription factor Com2. While the SSU1 gene and its activity have been correlated with sulfite tolerance, the work by Lage et al. (2019) indicates that Com2 might control a large percentage of the genes activated by SO2 and contribute to the yeast's protective response, offering new insights into the molecular factors influencing this oenological trait.

Targeting GATA transcription factors – a novel strategy for anti-aging interventions?

May 6, 2019

This article comments on work published by Carmona-Gutierrez et al. (Nat Commun., 2019), which identified a natural compound, 4,4'-dimethoxychalcone, inducing autophagy and prolonging lifespan in different organisms through a mechanism that involves GATA transcription factors.

Exploring the mechanism of amebic trogocytosis: the role of amebic lysosomes

December 6, 2017

In this article, the authors comment on the study "Inhibition of Amebic Lysosomal Acidification Blocks Amebic Trogocytosis and Cell Killing" by Gilmartin et al. (MBio, 2017), discussing the the role of amebic lysosomes in Trogocytosis, the intracellular transfer of fragments of cell material.

When a ribosomal protein grows up – the ribosome assembly path of Rps3

March 27, 2017

This article comments on two papers by Mitterer et al., which followed yeast protein Rps3, highlighting the sophisticated mechanisms for protein protection, nuclear transport, and integration into pre-ribosomal particles for final assembly with 40S subunits.

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