Research Articles – Page 9

Research, Research Articles

Depletion of SNAP-23 and Syntaxin 4 alters lipid droplet homeostasis during Chlamydia infection

Tiago Monteiro-Brás^1,2,3, Jordan Wesolowski¹ and Fabienne Paumet¹

This study reveals that the plasma membrane SNARE proteins SNAP-23 and Syntaxin 4 are crucial for Chlamydia trachomatis development by regulating lipid droplet homeostasis and supporting the formation of infectious progeny within host cells.

Yeast can express and assemble bacterial secretins in the mitochondrial outer membrane

Janani Natarajan¹, Anasuya Moitra¹, Sussanne Zabel^1,§, Nidhi Singh², Samuel Wagner^2,3 and Doron Rapaport¹

Secretins, essential components of bacterial secretion systems, can be expressed in yeast and show differential dependencies on mitochondrial import and assembly factors for membrane integration, suggesting diverse pathways for their assembly into the bacterial outer membrane.

Metabolic reprogramming of Salmonella infected macrophages and its modulation by iron availability and the mTOR pathway

Julia Telser^1,2,#, Chiara Volani^1,3,#, Richard Hilbe^1,2, Markus Seifert^1,2, Natascha Brigo¹, Giuseppe Paglia⁴ and Günter Weiss^1,2

This article shows that iron plays a critical role in both the immune response and metabolic reprogramming of macrophages during infection, influencing the TCA cycle and mTOR pathway, with implications for the growth of intracellular bacteria like Salmonella.

Type II-Metacaspases are involved in cell stress but not in cell death in the unicellular green alga Dunaliella tertiolecta

M. Teresa Mata^1,&, Armando Palma¹, Candela García-Gómez^1,#, María López-Parages¹, Víctor Vázquez¹, Iván Cheng-Sánchez², Francisco Sarabia², Félix López-Figueroa¹, Carlos Jiménez¹ and María Segovia¹

This article shows that in the green alga Dunaliella tertiolecta, Type-II metacaspases are involved in the stress response to ultraviolet radiation but are not linked to cell death, suggesting their role in survival strategies under stressful environmental conditions.

Transcriptomic and chemogenomic analyses unveil the essential role of Com2-regulon in response and tolerance of Saccharomyces cerevisiae to stress induced by sulfur dioxide

Patrícia Lage^1,2, Belém Sampaio-Marques^3,4, Paula Ludovico^3,4, Nuno P. Mira⁵ and Ana Mendes-Ferreira^1,2

This article shows that in the presence of sulfur dioxide (SO2), the transcription factor Com2 plays a critical role in the tolerance and response of Saccharomyces cerevisiae, affecting the expression of a majority of SO2-activated genes and contributing to the protection against stress induced by SO2 at an enologically relevant pH.

Proline metabolism regulates replicative lifespan in the yeast Saccharomyces cerevisiae

Yukio Mukai¹, Yuka Kamei¹, Xu Liu¹, Shan Jiang¹, Yukiko Sugimoto², Noreen Suliani binti Mat Nanyan², Daisuke Watanabe² and Hiroshi Takagi²

This article shows that intracellular proline levels in the budding yeast Saccharomyces cerevisiae are correlated with its replicative lifespan, suggesting a protective role of proline against cellular senescence due to various stresses.

Network dynamics of the yeast methyltransferome

Guri Giaever¹, Elena Lissina¹and Corey Nislow¹

This article presents a systematic genetic analysis of methyltransferases (MTases) under normal and stress conditions, uncovering the complex and adaptive nature of the methyltransferome and discovering a potential connection between phospholipid methylation and histone methylation, suggesting interplay between lipid homeostasis and epigenetic regulation.

Functional link between mitochondria and Rnr3, the minor catalytic subunit of yeast ribonucleotide reductase

Isaac Corcoles-Saez¹, Jean-Luc Ferat², Michael Costanzo³, Charles M. Boone³ and Rita S. Cha¹

This article shows that the carbon source affects the abundance of ribonucleotide reductase (RNR) subunits in yeast, with a novel Mec1 signaling axis regulating Rnr3 independently of known DNA damage response pathways, and reveals Rnr3's unexpected role in mitochondrial function.

Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release

Alexandre Légiot¹, Claire Céré¹, Thibaud Dupoiron¹, Mohamed Kaabouni¹, Nadine Camougrand¹ and Stéphen Manon¹

This study investigated the role of Mitochondria-Associated Membranes (MAMs) in the regulation of apoptosis by analyzing the localization and function of the pro-apoptotic protein Bax in yeast, finding that disruption of MAMs by deletion of the MDM34 gene affects Bax's mitochondrial localization and the release of cytochrome c.

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Broad-spectrum antifungal activities and mechanism of drimane sesquiterpenoids

March 12, 2020

This study identifies (-)-drimenol as a potent broad-spectrum antifungal agent effective against multiple pathogenic fungi, including drug-resistant strains, and reveals its mechanism of action involves disruption of fungal membranes and targeting Crk1-related pathways, with potential for structural optimization to enhance efficacy.

Stable and destabilized GFP reporters to monitor calcineurin activity in Saccharomyces cerevisiae

February 5, 2020

This study introduces GFP-based transcriptional reporters driven by a calcineurin-dependent response element, enabling real-time monitoring of calcineurin activity in live yeast cells for studying stress responses, aging, and antifungal drug screening.

The euchromatic histone mark H3K36me3 preserves heterochromatin through sequestration of an acetyltransferase complex in fission yeast

January 3, 2020

This study reveals that the loss of heterochromatin silencing in Set2-deficient cells is due to unrestrained Mst2C activity, highlighting the need for spatially restricted chromatin-modifying enzymes to maintain distinct chromatin states.