Regulation of extracellular vesicles for protein secretion in Aspergillus nidulans
This study reveals that Aspergillus nidulans boosts extracellular vesicle production when ER-trafficked enzymes are induced, uncovering how fungi remodel their secretome through vesicle-mediated secretion to adapt to changing environments and biofilm formation.
Transcriptomic response to different heme sources in Trypanosoma cruzi epimastigotes
This study uncovers how the Chagas disease parasite adapts to changes in heme, an essential molecule for its survival, providing transcriptional clues to heme metabolism and identifying a previously unreported heme-binding protein in T. cruzi.
Luminal acetylation of microtubules is not essential for Plasmodium berghei and Toxoplasma gondii survival
Acetylation of α-tubulin at lysine 40 is not essential for cytoskeletal stability in Plasmodium berghei or Toxoplasma gondii, suggesting redundancy and plasticity in microtubule regulation in these parasites.
The dual-site agonist for human M2 muscarinic receptors Iper-8-naphtalimide induces mitochondrial dysfunction in Saccharomyces cerevisiae
S. cerevisiae is a model to study human GPCRs. N-8-Iper, active against glioblastoma via M2 receptor, causes mitochondrial damage in yeast by binding Ste2, highlighting evolutionary conservation of GPCRs.
Integrative Omics reveals changes in the cellular landscape of peroxisome-deficient pex3 yeast cells
To uncover the consequences of peroxisome deficiency, we compared Saccharomyces cerevisiae wild-type with pex3 cells, which lack peroxisomes, employing quantitative proteomics and transcriptomics technologies.
Regulation of extracellular vesicles for protein secretion in Aspergillus nidulans
Rebekkah E. Pope1, Patrick Ballmann2, Lisa Whitworth3 and Rolf A. Prade1,*
This study reveals that Aspergillus nidulans boosts extracellular vesicle production when ER-trafficked enzymes are induced, uncovering how fungi remodel their secretome through vesicle-mediated secretion to adapt to changing environments and biofilm formation.
Transcriptomic response to different heme sources in Trypanosoma cruzi epimastigotes
Evelyn Tevere1,a, María G. Mediavilla1,a, Cecilia B. Di Capua1, Marcelo L. Merli1, Carlos Robello2,3, Luisa Berná2,4 and Julia A. Cricco
This study uncovers how the Chagas disease parasite adapts to changes in heme, an essential molecule for its survival, providing transcriptional clues to heme metabolism and identifying a previously unreported heme-binding protein in T. cruzi.
Sir2 regulates selective autophagy in stationary-phase yeast cells
Ji-In Ryua, Juhye Junga, and Jeong-Yoon Kim
This study establishes Sir2 as a previously unrecognized regulator of selective autophagy during the stationary phase and highlight how cells dynamically control organelle degradation.
Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress
Zhaojie Zhang1 and Gracie R. Zhang2
The budding yeast Saccharomyces cerevisiae is capable of surviving extreme water loss for a long time. However, less is known about the mechanism of its desiccation tolerance. In this study, we revealed that in an exponential culture, all desiccation tolerant yeast cells were in G1 phase and had condensed chromosomes. (…)
Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays
Mathieu Durand1, Philippe Thibault1, Simon Lévesque2,3, Ariane Brault4, Alex Carignan2, Louis Valiquette2, Philippe Martin2 and Simon Labbé4
The early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections is required to identify and isolate contagious patients to prevent further transmission of SARS-CoV-2. In this study, we present a multitarget real-time TaqMan reverse transcription PCR (rRT-PCR) assay for the quantitative detection of SARS-CoV-2 and some of its circulating variants harboring mutations that give the virus a selective advantage. Seven different primer-probe sets that included probes containing locked nucleic acid (LNA) nucleotides were designed to amplify specific wild-type and mutant sequences in Orf1ab, Envelope (E), Spike (S), and Nucleocapsid (N) genes (…)
Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking
Cinzia Klemm1,#, Henry Wood1,#, Grace Heredge Thomas1,#, Guðjón Ólafsson1,2, Mara Teixeira Torres1 and Peter H. Thorpe1
This work demonstrates that the yeast Synthetic Physical Interactions method is a rapid way to identify potential functions of ectopic viral proteins.
Airborne bacteria in show caves from Southern Spain
Irene Dominguez-Moñino1, Valme Jurado1, Miguel Angel Rogerio-Candelera1, Bernardo Hermosin1 and Cesareo Saiz-Jimenez1
This study analyzes the factors conditioning the diversity of airborne bacteria recorded in three Andalusian show caves, subjected to different managements.
Landscapes and bacterial signatures of mucosa-associated intestinal microbiota in Chilean and Spanish patients with inflammatory bowel disease
Nayaret Chamorro1,#, David A. Montero1,2,#, Pablo Gallardo3, Mauricio Farfán3, Mauricio Contreras4, Marjorie De la Fuente2, Karen Dubois2, Marcela A. Hermoso2, Rodrigo Quera5,6, Marjorie Pizarro-Guajardo7,8,9, Daniel Paredes-Sabja7,8,9, Daniel Ginard10, Ramon Rosselló-Móra11 and Roberto Vidal1,8,12
This study investigates the landscapes and alterations of mucosa-associated intestinal microbiota in patients with inflammatory bowel diseases, which cause chronic inflammation of the gut, including ulcerative colitis and Crohn’s disease.
Genome, transcriptome and secretome analyses of the antagonistic, yeast-like fungus Aureobasidium pullulans to identify potential biocontrol genes
Maria Paula Rueda-Mejia1, Lukas Nägeli1, Stefanie Lutz2, Richard D. Hayes3, Adithi R. Varadarajan2, Igor V. Grigoriev3,4, Christian H. Ahrens2,5 and Florian M. Freimoser1
This study highlights the value of a sequential approach starting with genome mining and consecutive transcriptome and secretome analyses in order to identify a limited number of potential target genes for detailed, functional analyses in Aureobasidium pullulans.
Proanthocyanidin-enriched cranberry extract induces resilient bacterial community dynamics in a gnotobiotic mouse model
Catherine C. Neto1,2,#, Benedikt M. Mortzfeld3,#, John R. Turbitt1,2, Shakti K. Bhattarai3, Vladimir Yeliseyev4, Nicholas DiBenedetto4, Lynn Bry4 and Vanni Bucci2,3
This study investigates the effect of a water-soluble, proanthocyanidin-rich cranberry juice extract on the short-term dynamics of a human-derived bacterial community in a gnotobiotic mouse model.
Dry biocleaning of artwork: an innovative methodology for Cultural Heritage recovery?
Giancarlo Ranalli1, Pilar Bosch-Roig2, Simone Crudele1, Laura Rampazzi3,4, Cristina Corti3 and Elisabetta Zanardini5
This work proposes an innovative methodology based on applied biotechnology for the recovery of altered stonework: the “dry biocleaning”, which envisages the use of dehydrated microbial cells without the use of free water or gel-based matrices.
New insights in the mode of action of anti-leishmanial drugs by using chemical mutagenesis screens coupled to next-generation sequencing
Arijit Bhattacharya1, Sophia Bigot2, Prasad Kottayil Padmanabhan2, Angana Mukherjee2, Adriano Coelho3, Philippe Leprohon2, Barbara Papadopoulou2 and Marc Ouellette2
In this article, the authors comment on the study “Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania” by Bhattacharya et al. (Nat Commun, 2019), which introduces Mut-seq, a chemical mutagenesis and sequencing approach, to uncover drug resistance mechanisms in Leishmania, revealing links between lipid metabolism genes and miltefosine resistance, and a protein kinase involved in translation conferring paromomycin resistance.
Microfluidic techniques for separation of bacterial cells via taxis
Jyoti P. Gurung1, Murat Gel2,3 and Matthew A. B. Baker1,3
Microfluidic tools, ideal for studying microbial motility due to their control over laminar flows at microscopic scales, enable precise analysis of various taxis behaviors and have advanced applications in synthetic biology, directed evolution, and medical microbiology.
Influence of delivery and feeding mode in oral fungi colonization – a systematic review
Maria Joao Azevedo1,2,3,4, Maria de Lurdes Pereira1,5, Ricardo Araujo2,3,6, Carla Ramalho3,7,8, Egija Zaura4 and Benedita Sampaio-Maia1,2,3
A systematic review of oral fungal colonization in infants found that while breastfeeding did not significantly affect the oral mycobiome, vaginal delivery was associated with higher oral yeast colonization, particularly of Candida albicans.
A holobiont view on thrombosis: unravelling the microbiota’s influence on arterial thrombus growth
Giulia Pontarollo1, Klytaimnistra Kiouptsi1 and Christoph Reinhardt1,2
In this article, the authors comment on the study “The microbiota promotes arterial thrombosis in low-density lipoprotein receptor-deficient mice” by Kiouptsi et al. (mBio, 2019) that showed that commensal microbiota, intricately linked to host physiology, may influence cardiovascular disease, as shown by studies using germ-free atherosclerosis-prone mice to examine how microbial presence and diet affect arterial thrombosis and lesion development.
The role of Lactobacillus species in the control of Candida via biotrophic interactions
Isabella Zangl1, Ildiko-Julia Pap2, Christoph Aspöck2 and Christoph Schüller1,3
Microbial communities, including Candida and Lactobacillus species, play a crucial role in human health, particularly in the context of mucosal infections, but our understanding of their interactions and effects is still incomplete due to the variability of species and isolates as well as the complexity of the human host.
Tribal warfare: Commensal Neisseria kill pathogen Neisseria gonorrhoeae using its DNA
Magdalene So1 and Maria A. Rendón1
This article comments on work published by Kim et al (Cell Host Microbe, 2019), which adds a new dimension to the concept of commensal protection. It shows that commensal Neisseria kill the closely related pathogen N. gonorrhoeae through an unexpected mechanism, one that involves genetic competence, DNA methylation state and recombination.
Yet another job for the bacterial ribosome
Andrea Origi1,2, Ana Natriashivili1,2, Lara Knüpffer1, Clara Fehrenbach1, Kärt Denks1,2, Rosella Asti1 and Hans-Georg Koch1
This article comments on work published by Knüpffer et al (mBio, 2019), which revealed the intricate interaction of uL23 with yet another essential player in bacteria, the ATPase SecA, which is best known for its role during post-translational secretion of proteins across the bacterial SecYEG translocon
Gut microbial metabolites in depression: understanding the biochemical mechanisms
Giorgia Caspani1, Sidney Kennedy2-5, Jane A. Foster6 and Jonathan Swann1
This article shows how the gut microbiota contributes to the pathophysiology of depression and examines the mechanisms by which microbially-derived molecules may influence depressive behavior, highlighting the potential of dietary interventions as novel therapeutic strategies.
The multiple functions of the numerous Chlamydia trachomatis secreted proteins: the tip of the iceberg
Joana N. Bugalhão1 and Luís Jaime Mota1
CThis article shows an in-depth review on the current knowledge and outstanding questions about secreted proteins from Chlamydia trachomatis, detailing their roles in host cell interaction and immune response evasion.
Targeting GATA transcription factors – a novel strategy for anti-aging interventions?
Andreas Zimmermann1, Katharina Kainz1,2, Sebastian J. Hofer1,3, Maria A. Bauer1, Sabrina Schroeder1, Jörn Dengjel4, Federico Pietrocola5, Oliver Kepp6-9, Christoph Ruckenstuhl1, Tobias Eisenberg1,3,10,11, Stephan J. Sigrist12, Frank Madeo1,3,10, Guido Kroemer6-9, 13-15 and Didac Carmona-Gutierrez1
This article comments on work published by Carmona-Gutierrez et al. (Nat Commun., 2019), which identified a natural compound, 4,4′-dimethoxychalcone, inducing autophagy and prolonging lifespan in different organisms through a mechanism that involves GATA transcription factors.
In the beginning was the word: How terminology drives our understanding of endosymbiotic organelles
Miroslav Oborník 1,2
This In the Pit article argues that the naming conventions for biological entities influence research perspectives and methodologies, advocating for mitochondria and plastids to be classified and named as bacteria due to their endosymbiotic origins, with potential implications for our understanding of bacterial prevalence, definitions of the microbiome and multicellularity, and the concept of endosymbiotic domestication.
What’s in a name? How organelles of endosymbiotic origin can be distinguished from endosymbionts
Ansgar Gruber1
This In the Pit article suggests redefining the relationship between hosts and endosymbionts, like mitochondria and plastids, as a single species based on “sexual symbiont integration,” the loss of independent speciation, and congruence in genetic recombination and population sizes, rather than solely on historic classifications or structural properties.
Microbial wars: competition in ecological niches and within the microbiome
Maria A. Bauer1, Katharina Kainz1, Didac Carmona-Gutierrez1 and Frank Madeo1,2
In this Editorial Bauer et al. provide a brief overview on microbial competition and discuss some of its roles and consequences that directly affect humans.
Exploring the mechanism of amebic trogocytosis: the role of amebic lysosomes
Allissia A. Gilmartin1 and William A. Petri, Jr1,2,3
In this article, the authors comment on the study “Inhibition of Amebic Lysosomal Acidification Blocks Amebic Trogocytosis and Cell Killing” by Gilmartin et al. (MBio, 2017), discussing the the role of amebic lysosomes in Trogocytosis, the intracellular transfer of fragments of cell material.
Uncovering the hidden: complexity and strategies for diagnosing latent tuberculosis
Mario Alberto Flores-Valdez
This editorial postulates that advanced proteomic and transcriptomic techniques are evolving and may enhance the detection of latent tuberculosis, thereby distinguishing true M. tuberculosis infections from other conditions, which is vital for controlling potential reactivation and transmission.
The Yin & Yang of Mitochondrial Architecture – Interplay of MICOS and F1Fo-ATP synthase in cristae formation
Heike Rampelt1 and Martin van der Laan2
This Editorial posits that mitochondrial cristae architecture is shaped by the interplay of MICOS and ATP synthase, with a recent study illuminating their roles in cristae formation and maintenance.
When a ribosomal protein grows up – the ribosome assembly path of Rps3
Brigitte Pertschy
This article comments on two papers by Mitterer et al., which followed yeast protein Rps3, highlighting the sophisticated mechanisms for protein protection, nuclear transport, and integration into pre-ribosomal particles for final assembly with 40S subunits.
Microbial Cell
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Peer-reviewed, open-access research using unicellular organisms (and multicellular microorganisms) to understand cellular responses and human disease.
The journal (founded in 2014) is led by its Editors-in-Chief Frank Madeo, Didac Carmona-Gutierrez, and Guido Kroemer
Microbial Cell has been publishing original scientific literature since 2014, and from the very beginning has been managed by active scientists through an independent Publishing House (Shared science Publishers). The journal was conceived as a platform to acknowledge the importance of unicellular organisms, both as model systems as well as in the biological context of human health and disease.
Ever since, Microbial Cell has very positively developed and strongly grown into a respected journal in the unicellular research community and even beyond. This scientific impact is reflected in the yearly number of citations obtained by articles published in Microbial Cell, as recorded by the Web of Science (Clarivate, formerly Thomson/Reuters):
The scientific impact of Microbial Cell is also mirrored in a series of milestones:
2015: Microbial Cell is included in the Emerging Sources Citation Index (ESCI), a selection of developing journals drafted by Clarivate Analytics based on the candidate’s publishing standards, quality, editorial content, and citation data. Note: As an ESCI-selected journal, Microbial Cell is currently being evaluated in a rigorous and long process to determine an inclusion in the Science Citation Index Expanded (SCIE), which allows the official calculation of Clarivate Analytics’ impact factor.
2016: Microbial Cell is awarded the so-called DOAJ Seal by the selective Directory of Open Access Journals (DOAJ). The DOAJ Seal is an exclusive mark of certification for open access journals granted by DOAJ to journals that adhere to outstanding best practice and achieve an extra high and clear commitment to open access and high publishing standards.
2017: Microbial Cell is included in Pubmed Central (PMC), allowing the archiving of all the journal’s articles in PMC and PubMed.
2019: Microbial Cell is indexed in the prestigious abstract and citation database Scopus after a thorough selection process. This also means that Microbial Cell obtains, for the first time, an official Scopus CiteScore as well as an official journal ranking in the Scimago Journal and Country Ranking.
2022: Microbial Cell’s CiteScore reaches a value of 7.2 for the year 2021, positioning Microbial Cell among the top microbiology journals (previously available CiteScores: 2019: 5.4; 2020: 5.1).
2022: Microbial Cell is indexed in the highly selective Science Citation Index Expanded™, which covers approx. 9,500 of the world’s most impactful journals across 178 scientific disciplines. In their journal selection and curation process, Clarivate´s editors apply 24 ‘quality’ criteria and four ‘impact’ criteria to select the most influential journals in their respective fields. This selection is also a pre-requisite for inclusion in the JCR, which features the impact factor.
2022: Microbial Cell is listed in the Journal Citation Reports™ (JCR), and obtains its first official Journal Impact Factor™ (JIF) for the year 2021: 5.316.Check Article Types and Manuscript Preparation guidelines. Submit online via Scholastica.
Sulfur dioxide resistance in Saccharomyces cerevisiae: beyond SSU1
Estéfani García-Ríos1 and José Manuel Guillamón1
This article discusses the importance of understanding sulfite resistance in Saccharomyces cerevisiae due to its use in winemaking and the potential role of the transcription factor Com2. While the SSU1 gene and its activity have been correlated with sulfite tolerance, the work by Lage et al. (2019) indicates that Com2 might control a large percentage of the genes activated by SO2 and contribute to the yeast’s protective response, offering new insights into the molecular factors influencing this oenological trait.