Vol. 01, 2014

On the link between cell cycle and infection of the Alphaproteobacterium Brucella abortus

Michaël Deghelt, Jean-Jacques Letesson, Xavier De Bolle

This article comments on work published by Deghelt et al. (Nat Comm, 2014), which describe a cell cycle arrest and resume during the Brucella abortus trafficking in host cell, suggesting that like the model Alphaproteobacterium Caulobacter crescentus, these bacteria are able to block their cell cycle at the G1 phase when starvation is sensed.

Divide and conquer: processive transport enables multidrug transporters to tackle challenging drugs

Nir Fluman and Eitan Bibi

This article comments on work published by Fluman et al. (Nat Comm, 2014), which describes the ability of bacterial multidrug transporters to move long molecules through the membrane in a processive manner.

The dual role of cyclin C connects stress regulated gene expression to mitochondrial dynamics

Randy Strich and Katrina F. Cooper

This work summarizes the role cyclin C plays in regulating stress-responsive transcription in the budding yeast Saccharomyces cerevisiae, including mitochondrial fission and regulated cell death.

Combinatorial stress responses: direct coupling of two major stress responses in Escherichia coli

Daniel R. Brown, Geraint Barton, Zhensheng Pan, Martin Buck and Sivaramesh Wigneshweraraj

This article comments on work published by Brown et al. (Nat Comm, 2014), which showed that the transcription of relA is activated by NtrC during nitrogen starvation, revealing that in E. coli and related bacteria, NtrC functions in combinatorial stress and serves to couple two major stress responses, the Ntr response and stringent response.

Time resolved DNA occupancy dynamics during the respiratory oscillation uncover a global reset point in the yeast growth program

Cornelia Amariei, Rainer Machné, Viktor Stolc, Tomoyoshi Soga, Masaru Tomita and Douglas B. Murray

Using multiple approaches, this work implies a nucleosome focusing event as a key step that resets transcription during the respiratory oscillation.

Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae

António Rego#, Ana Marta Duarte#, Flávio Azevedo#, Maria João Sousa, Manuela Côrte-Real and Susana R. Chaves

This work characterizes the involvement of MAPK signaling pathways in cell death induced by acetic acid in Saccharomyces cerevisiae.

Metabolic pathways further increase the complexity of cell size control in budding yeast

Jorrit M. Enserink

This article comments on work published by Soma et al. (Microbial Cell, 2014), which teased apart the effect of metabolism and growth rate on setting of critical cell size in Saccharomyces cerevisiae.

Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death in Neurospora crassa

A. Pedro Gonçalves1,2, João Monteiro2, Chiara Lucchi2, David J. Kowbel3, J. Miguel Cordeiro1,4, Paulo Correia-de-Sá1,4, Daniel J. Rigden5, N. Louise Glass3, Arnaldo Videira1,2

The results presented here reveal that in Neurospora crassa, extracellular Ca2+ modulates cell death and the transcriptional alterations induced by staurosporine, and lead to the identification of two novel putative Ca2+-binding proteins, encoded by the NCU08524 and NCU06607 genes.

Multiple metabolic requirements for size homeostasis and initiation of division in Saccharomyces cerevisiae

Shivatheja Soma, Kailu Yang, Maria I. Morales and Michael Polymenis

This article reveals an unexpected diversity in the G1 cell cycle phenotypes of metabolic and biosynthetic Saccharomyces cerevisiae mutants, suggesting that growth requirements for cell division are multiple, distinct and imposed throughout the G1 phase of the cell cycle.

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Plasmodium spp. membrane glutathione S-transferases: detoxification units and drug targets

Andreas Martin Lisewski

This article comments on work published by Lisewski et al. (Cell, 2014), which reported the first examples of membrane-associated proteins in eicosanoid and glutathione metabolism members among Plasmodium spp.

Proline cis-trans isomerization is influenced by local lysine acetylation-deacetylation

Françoise S. Howe and Jane Mellor

This article comments on work published by Howe et al. (Mol Cell, 2014), which shows that local lysine acetylation and deacetylation modulate proline cis-trans isomerization in Saccharomyces cerevisiae.

An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids

Harriet Allison1, Amanda J. O’Reilly1, Jeremy Sternberg2 and Mark C. Field1

This work describes a novel family of type I membrane proteins ("invariant glycoproteins") and proposes them as trypanosomatid-specific ER-localised glycoproteins, with potential contributions to life cycle progression and immunity, that utilise oligomerisation as an ER retention mechanism.

On the link between cell cycle and infection of the Alphaproteobacterium Brucella abortus

Michaël Deghelt, Jean-Jacques Letesson, Xavier De Bolle

This article comments on work published by Deghelt et al. (Nat Comm, 2014), which describe a cell cycle arrest and resume during the Brucella abortus trafficking in host cell, suggesting that like the model Alphaproteobacterium Caulobacter crescentus, these bacteria are able to block their cell cycle at the G1 phase when starvation is sensed.

Divide and conquer: processive transport enables multidrug transporters to tackle challenging drugs

Nir Fluman and Eitan Bibi

This article comments on work published by Fluman et al. (Nat Comm, 2014), which describes the ability of bacterial multidrug transporters to move long molecules through the membrane in a processive manner.

The dual role of cyclin C connects stress regulated gene expression to mitochondrial dynamics

Randy Strich and Katrina F. Cooper

This work summarizes the role cyclin C plays in regulating stress-responsive transcription in the budding yeast Saccharomyces cerevisiae, including mitochondrial fission and regulated cell death.

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A non-proteolytic function of ubiquitin in transcription repression

Ada Ndoja and Tingting Yao

This article comments on work published by Ndoja et al. (Mol Cell, 2014), which demonstrates that monoubiquitination of some transcription activators can inhibit transcription by recruiting the AAA+ ATPase Cdc48 (also known in metazoans as p97 or VCP), which then extracts the ubiquitinated activator from DNA.

Where antibiotic resistance mutations meet quorum-sensing

Rok Krašovec1, Roman V. Belavkin2, John A.D. Aston3, Alastair Channon4, Elizabeth Aston4, Bharat M. Rash1, Manikandan Kadirvel5,6, Sarah Forbes6, and Christopher G. Knight1

This article comments on work published by Krašovec et al. (Nat Comm, 2014), which found that the modulation of de novo mutation to promote antibiotic resistance depends on the density of the bacterial population and cell-cell interactions (rather than, for instance, the level of stress).

Sphingolipids and mitochondrial function, lessons learned from yeast

Pieter Spincemaille1, Bruno P.A. Cammue1,2 and Karin Thevissen1

This article reviews recent research showing that Saccharomyces cerevisiae is an invaluable model to investigate sphingolipids as signaling molecules in modulating mitochondrial function, but can also be used as a tool to further enhance our current knowledge on sphingolipids and mitochondria in mammalian cells.

Genome evolution in yeast reveals connections between rare mutations in human cancer

Xinchen Teng1,2 and J. Marie Hardwick2

This article comments on work published by Teng et al. (Mol Cell, 2013), which, using the yeast knockout collections, provides hard evidence that single gene deletions/mutations in most non-essential genes can drive the selection for cancer-like mutations.

Cell-autonomous mechanisms of chronological aging in the yeast Saccharomyces cerevisiae

Anthony Arlia-Ciommo#, Anna Leonov#, Amanda Piano#, Veronika Svistkova# and Vladimir I. Titorenko

This article critically analyzes recent advances in the understanding of cell-autonomous mechanisms of chronological aging in the budding yeast Saccharomyces cerevisiae. It proposes a concept of a biomolecular network underlying the chronology of cellular aging in yeast, whichposits that such network progresses through a series of lifespan checkpoints.

Decoding the biosynthesis and function of diphthamide, an enigmatic modification of translation elongation factor 2 (EF2)

Raffael Schaffrath and Michael J. R. Stark

This article comments on work published by Uthman et al. (PLoS Genet, 2013), which suggests that Dph5 has a novel role as an EF2 inhibitor that affects cell growth when diphthamide synthesis is blocked or incomplete and shows that diphthamide promotes the accuracy of EF2 performance during translation.

Autophagy extends lifespan via vacuolar acidification

Christoph Ruckenstuhl1, Christine Netzberger1, Iryna Entfellner1, Didac Carmona-Gutierrez1, Thomas Kickenweiz1, Slaven Stekovic1, Christina Gleixner1, Christian Schmid1, Lisa Klug1, Ivan Hajnal1, Alice G. Sorgo1, Tobias Eisenberg1, Sabrina Büttner1, Guillermo Marin͂o2-4,  Rafal Koziel5, Christoph Magnes6, Frank Sinner6,7, Thomas R. Pieber6,7, Pidder Jansen-Dürr5, Kai-Uwe Fröhlich1, Guido Kroemer2,3,8-11, and Frank Madeo1

This article comments on work published by Ruckenstuhl et al. (PLoS Genet, 2014), which uses Saccharomyces cerevisiae to show that autophagy promotes lifespan extension upon MetR and requires the subsequent stimulation of vacuolar acidification, while it is epistatic to the equally autophagy-dependent anti-aging pathway triggered by TOR1 inhibition or deletion.

When less is more: hormesis against stress and disease

Andreas Zimmermann1, Maria A. Bauer1, Guido Kroemer2-5, Frank Madeo1 and Didac Carmona-Gutierrez1

This article condenses the conceptual and potentially therapeutic importance of hormesis by providing a short overview of current evidence in favor of the cytoprotective impact of hormesis, as well as of its underlying molecular mechanisms.

Morphed and moving: TNFα-driven motility promotes cell dissemination through MAP4K4-induced cytoskeleton remodeling

Min Ma1,2 and Martin Baumgartner1

This article comments on work published by Ma and Baumgartner (PLoS Patho, 2014), which investigated Theileria parasite control of host cell motile properties in the context of inflammatory signaling.

Metabolic pathways further increase the complexity of cell size control in budding yeast

Jorrit M. Enserink

This article comments on work published by Soma et al. (Microbial Cell, 2014), which teased apart the effect of metabolism and growth rate on setting of critical cell size in Saccharomyces cerevisiae.

Only functional localization is faithful localization

Roland Lill1,2,3

This article comments on work published by Peleh et al. (Microbial Cell 2014), which analyzes the localization of Dre2 in Saccharomyces cerevisiae.

Metabolites in aging and autophagy

Sabrina Schroeder1,#, Andreas Zimmermann1,#, Didac Carmona-Gutierrez1, Tobias Eisenberg1, Christoph Ruckenstuhl1, Aleksandra Andryushkova1, Tobias Pendl1, Alexandra Harger1,2 and Frank Madeo1

This article analyzes the implications of specific metabolites in aging and autophagy with special emphasis on polyamine metabolism.

One cell, one love: a journal for microbial research

Didac Carmona-Gutierrez1, Guido Kroemer2-6 and Frank Madeo1

In this inaugural article of Microbial Cell, we highlight the importance of microbial research in general and the journal's intention to serve as a publishing forum that supports and enfolds the scientific diversity in this area as it provides a unique, high-quality and universally accessible source of information and inspiration.

What’s the role of autophagy in trypanosomes?

Katherine Figarella1 and Néstor L. Uzcátegui1,2

This article comments on Proto et al. (Microbial Cell, 2014), who report first insights into the molecular mechanism of autophagy in African trypanosomes by generating reporter bloodstream form cell lines.

At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations

May 20, 2014

The data presented in this work indicate that in H. polymorpha at neutral pH the chronological lifespan invariably extends upon increasing the carbon source concentration.

Autophagy extends lifespan via vacuolar acidification

May 5, 2014

This article comments on work published by Ruckenstuhl et al. (PLoS Genet, 2014), which uses Saccharomyces cerevisiae to show that autophagy promotes lifespan extension upon MetR and requires the subsequent stimulation of vacuolar acidification, while it is epistatic to the equally autophagy-dependent anti-aging pathway triggered by TOR1 inhibition or deletion.

When less is more: hormesis against stress and disease

May 5, 2014

This article condenses the conceptual and potentially therapeutic importance of hormesis by providing a short overview of current evidence in favor of the cytoprotective impact of hormesis, as well as of its underlying molecular mechanisms.

Morphed and moving: TNFα-driven motility promotes cell dissemination through MAP4K4-induced cytoskeleton remodeling

April 24, 2014

This article comments on work published by Ma and Baumgartner (PLoS Patho, 2014), which investigated Theileria parasite control of host cell motile properties in the context of inflammatory signaling.

Hormesis: a fundamental concept in biology

April 23, 2014

This article addresses the concept of hormetic dose response, which describes the limits to which integrative endpoints can be modulated (i.e., enhanced or diminished) by pharmaceutical, chemical and physical means.

Live longer on MARS: a yeast paradigm of mitochondrial adaptive ROS signaling in aging

April 23, 2014

In this article, the potential relevance of Mitochondrial Adaptive ROS Signaling (MARS) to the human disease Ataxia-Telangiectasia and as a potential anti-aging target is discussed.

Prokaryotic Argonautes – variations on the RNA interference theme

April 15, 2014

This article comments on work published by Swarts et al. (Nature, 2014), which demonstrates that Argonaute family protein of the bacterium Thermus thermophilus acts as a barrier for the uptake and propagation of foreign DNA.

Longevity pathways and maintenance of the proteome: the role of autophagy and mitophagy during yeast ageing

April 7, 2014

This review describes recent findings that shed light on how longevity pathways and metabolic status impact maintenance of the proteome in both yeast ageing paradigms. These findings demonstrate that yeast remain a powerful model system for elucidating these relationships and their influence on ageing regulation.

Only functional localization is faithful localization

April 7, 2014

This article comments on work published by Peleh et al. (Microbial Cell 2014), which analyzes the localization of Dre2 in Saccharomyces cerevisiae.

Metabolites in aging and autophagy

April 7, 2014

This article analyzes the implications of specific metabolites in aging and autophagy with special emphasis on polyamine metabolism.