Review, Reviews

Bacterial maze runners reveal hidden diversity in chemotactic performance

M. Mehdi Salek1,#, Francesco Carrara1,#, Vicente Fernandez1 and Roman Stocker1

This article comments on work published by Salek et al. (Nat Commun, 2019), which combined microfluidic experiments with mathematical modeling to demonstrate that even in clonal populations, bacteria are individuals with different abilities to climb chemical gradients.

Beyond cells – The virome in the human holobiont

Rodrigo García-López1,2,3, Vicente Pérez-Brocal1,2,3 and Andrés Moya1,2,3

This article provides an overview of viromics—viral metagenomics—and its evolution, highlighting the complexity and dynamic nature of viruses beyond their traditional view as pathogens. It acknowledges the technological advancements in molecular biology and sequencing that have allowed the field to grow, while also noting that viromic research still faces significant challenges and is not as developed as bacterial metagenomics. The review underscores the importance of viruses in various ecosystems, their role in shaping the genetic landscape, and their potential impact, and looks forward to future directions in viromic research.

Laundry and textile hygiene in healthcare and beyond

Dirk P. Bockmühl1, Jan Schages1 and Laura Rehberg1

This article shows that while institutional laundering is regulated to ensure hygiene, the trend towards energy-efficient washing at lower temperatures raises concerns about the antimicrobial efficacy of domestic laundering, with a focus on addressing microbial contamination in both clinical and home settings.

Bacterial pathogens under high-tension: Staphylococcus aureus adhesion to von Willebrand factor is activated by force

Felipe Viela1, Pietro Speziale2,3, Giampiero Pietrocola2 and Yves F. Dufrêne1,4

This article comments on work published by Viela et al (mBio, 2019), which shows that the bacterial cell surface protein A to the large plasma glycoprotein von Willebrand factor interaction is tightly regulated by mechanical force.

Yeast AP-1 like transcription factors (Yap) and stress response: a current overview

Claudina Rodrigues-Pousada1, Frédéric Devaux2, Soraia M. Caetano1, Catarina Pimentel1, Sofia da Silva1, Ana Carolina Cordeiro1 and Catarina Amaral1

This review summarizes current understanding of the eight Yap transcription factors in Saccharomyces cerevisiae, detailing their activation by specific stress conditions and discussing their function and evolution across various fungal species.

Septin clearance from the division site triggers cytokinesis in budding yeast

Davide Tamborrini1 and Simonetta Piatti1

This article comments on work published by Tamborrini et al (Nat Commun., 2019), which shows that septin displacement during splitting is an essential prerequisite for contractile actomyosin ring constriction during mitosis.

The influence of the microbiota on immune development, chronic inflammation, and cancer in the context of aging

Taylor N. Tibbs1,#, Lacey R. Lopez1,#, and Janelle C. Arthur1,2,3

This article shows that the microbiota is crucial for immune system development and that its relationship with the immune system during aging and the pathogenesis of age-related diseases, including cancer, needs further research to inform disease treatment and prevention.

Ser/Thr protein phosphatases in fungi: structure, regulation and function

Joaquín Ariño1, Diego Velázquez1 and Antonio Casamayor1

In this work we present the members of this family in S. cerevisiae and other fungal species, and review the most recent findings concerning their regulation and the roles they play in the most diverse aspects of cell biology.

Forty-five-year evolution of probiotic therapy

Scarlett Puebla-Barragan1,2 and Gregor Reid1,2

The field of probiotics has greatly expanded over the past 45 years, driven by the need for safer alternatives to drugs, interest in natural microbial products, and clinical proof of effectiveness, with scientific formulations increasingly defining the market and promising applications for various health areas expected in the future.

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Functions and regulation of the MRX complex at DNA double-strand breaks

July 27, 2016

DNA double-strand breaks (DSBs) pose a serious threat to genome stability and cell survival. Cells possess mechanisms that recognize DSBs and promote their repair through either homologous recombination (HR) or non-homologous end joining (NHEJ). The present review focuses mainly on recent works in the budding yeast Saccharomyces cerevisiae to highlight structure and regulation of the evolutionary conserved Mre11-Rad50-Xrs2 (MRX) complex as well as its interplays with Tel1.

Syphilis: Re-emergence of an old foe

June 27, 2016

Syphilis is caused by infection with Treponema pallidum subsp. pallidum, a not-yet-cultivable spiral-shaped bacterium that is usually transmitted by sexual contact with an infected partner or by an infected pregnant woman to her fetus. This review provides insights into the etiology, epidemiology, clinical manifestation, diagnosis, treatment and prevention of syphilis.

Genital Herpes: Insights into Sexually Transmitted Infectious Disease

June 27, 2016

Genital herpes is one of the most common, persistent and highly infectious sexually transmitted viral infections. This review provides an insight into the epidemiology, pathology, our current understanding of the molecular mechanisms of infection and the currently available and upcoming treatments for genital herpes.

Trichomoniasis – are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide?

June 27, 2016

Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in the world. This article contributes to claim the attention of public health policies to control this STD.

Evidence for the hallmarks of human aging in replicatively aging yeast

June 20, 2016

Recently, efforts have been made to characterize the hallmarks that accompany and contribute to the phenomenon of aging, as most relevant for humans. Remarkably, studying the finite lifespan of the single cell eukaryote budding yeast has been paramount for our understanding of aging. Here, we compile observations from literature over the past decades of research on replicatively aging yeast to highlight how the hallmarks of aging in humans are present in yeast.

The molecular and cellular action properties of artemisinins: what has yeast told us?

April 14, 2016

Artemisinin (ART) or Qinghaosu is a natural compound possessing superior anti-malarial activity. Although intensive studies have been done in the medicinal chemistry field to understand the structure-effect relationship, the biological actions of artemisinin are poorly understood and controversial. This review summarizes what we have learned from yeast about the basic biological properties of ARTs, as well as some key unanswered questions.

Chromatin binding and silencing: Two roles of the same protein Lem2

April 4, 2016

This article comments on work published by Barrales et al. (Genes Dev, 2016), which identifies the nuclear envelope protein Lem2, a homolog of metazoan lamin-associated proteins (LAPs), as a relevant factor for heterochromatin silencing and perinuclear localization in the fission yeast Schizosaccharomyces pombe.

Signaling pathways and posttranslational modifications of tau in Alzheimer’s disease: the humanization of yeast cells

March 27, 2016

In the past decade, yeast have been frequently employed to study the molecular mechanisms of human neurodegenerative diseases, generally by means of heterologous expression of genes encoding the relevant hallmark proteins. Substantial posttranslational modifications of many of these proteins are required for the development and progression of potentially disease relevant changes. We give an overview on common modifications as they occur in tau during AD and discuss potential approaches to humanize yeast in order to create modification patterns resembling the situation in mammalian cells.

Histone modifications as regulators of life and death in Saccharomyces cerevisiae

December 31, 2015

The mechanism by which chromosomes restructure during apoptosis is still poorly understood, but it is becoming increasingly clear that altered epigenetic histone modifications are fundamental parameters that influence the chromatin state and the nuclear rearrangements within apoptotic cells. This review highlights recent work on the epigenetic regulation of programmed cell death in budding yeast.

Electron microscopy for ultrastructural analysis and protein localization in Saccharomyces cerevisiae

October 12, 2015

The yeast Saccharomyces cerevisiae is a key model system for studying of a multitude of cellular processes because of its amenability to genetics, molecular biology and biochemical procedures. The goal of this review is to guide researchers that want to investigate a particular process at the ultrastructural level in yeast by aiding in the selection of the most appropriate approach to visualize a specific structure or subcellular compartment.

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