Commentary, Viewpoint

The emerging role of complex modifications of tRNALysUUU in signaling pathways

Patrick C. Thiaville1,2,3,4 and Valérie de Crécy-Lagard2,4

This comment discusses the article "Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling" by Scheidt et al (Microbial Cell, 2014).

Metabolic pathways further increase the complexity of cell size control in budding yeast

Jorrit M. Enserink

This article comments on work published by Soma et al. (Microbial Cell, 2014), which teased apart the effect of metabolism and growth rate on setting of critical cell size in Saccharomyces cerevisiae.

Only functional localization is faithful localization

Roland Lill1,2,3

This article comments on work published by Peleh et al. (Microbial Cell 2014), which analyzes the localization of Dre2 in Saccharomyces cerevisiae.

Metabolites in aging and autophagy

Sabrina Schroeder1,#, Andreas Zimmermann1,#, Didac Carmona-Gutierrez1, Tobias Eisenberg1, Christoph Ruckenstuhl1, Aleksandra Andryushkova1, Tobias Pendl1, Alexandra Harger1,2 and Frank Madeo1

This article analyzes the implications of specific metabolites in aging and autophagy with special emphasis on polyamine metabolism.

One cell, one love: a journal for microbial research

Didac Carmona-Gutierrez1, Guido Kroemer2-6 and Frank Madeo1

In this inaugural article of Microbial Cell, we highlight the importance of microbial research in general and the journal's intention to serve as a publishing forum that supports and enfolds the scientific diversity in this area as it provides a unique, high-quality and universally accessible source of information and inspiration.

What’s the role of autophagy in trypanosomes?

Katherine Figarella1 and Néstor L. Uzcátegui1,2

This article comments on Proto et al. (Microbial Cell, 2014), who report first insights into the molecular mechanism of autophagy in African trypanosomes by generating reporter bloodstream form cell lines.

Previous

Patterns of protein synthesis in the budding yeast cell cycle: variable or constant?

August 20, 2024

Proteins are the principal macromolecular constituent of proliferating cells, and protein synthesis is viewed as a primary metric of cell growth. While there are celebrated examples of proteins whose levels are periodic in the cell cycle (e.g., cyclins), the concentration of most proteins was not thought to change in the cell cycle, but some recent results challenge this notion. The ‘bulk’ protein is the focus of this article, specifically the rate of its synthesis, in the budding yeast Saccharomyces cerevisiae.

Ribose 5-phosphate: the key metabolite bridging the metabolisms of nucleotides and amino acids during stringent response in Escherichia coli?

June 1, 2023

Here we propose the metabolite ribose 5’-phosphate as the key link between nucleotide and amino acid metabolisms and a working model integrating both the transcriptional and metabolic effects of (p)ppGpp on E. coli physiological adaptation during the stringent response.

Means of intracellular communication: touching, kissing, fusing

April 13, 2021

This work highlights different aspects of communication between organelles, including the importance of organellar contact sites.

Regulation of the mitochondrial permeability transition pore and its effects on aging

June 22, 2020

Aging is linked to mitochondrial function, with the mitochondrial permeability transition pore (mPTP) playing a key role. Yeast is a useful model for studying how mPTP affects cell survival, aging, and related diseases.

Digesting the crisis: autophagy and coronaviruses

May 4, 2020

This article reviews the multifaceted role of autophagy in antiviral defense and highlights how coronaviruses, including SARS-CoV-2, interact with this pathway, raising the possibility that targeting autophagy could offer novel therapeutic strategies against COVID-19.

Physiology, phylogeny, and LUCA

November 25, 2016

Genomes record their own history. But if we want to look all the way back to life's beginnings some 4 billion years ago, the record of microbial evolution that is preserved in prokaryotic genomes is not easy to read. The classical approach has been to look for genes that are universally distributed. Another approach is to make all trees for all genes, and sift out the trees where signals have been overwritten by lateral gene transfer. What is left ought to be ancient. If we do that, what do we find?

Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation.

August 1, 2016

Diploid budding yeast (Saccharomyces cerevisiae) can adopt one of several alternative differentiation fates in response to nutrient limitation, and each of these fates provides distinct biological functions. When different strain backgrounds are taken into account, these various fates occur in response to similar environmental cues, are regulated by the same signal transduction pathways, and share many of the same master regulators. I propose that the relationships between fate choice, environmental cues and signaling pathways are not Boolean, but involve graded levels of signals, pathway activation and master-regulator activity.

Yeast proteinopathy models: a robust tool for deciphering the basis of neurodegeneration

November 26, 2015

Protein quality control or proteostasis is an essential determinant of basic cell health and aging. Eukaryotic cells have evolved a number of proteostatic mechanisms to ensure that proteins retain functional conformation, or are rapidly degraded when proteins misfold or self-aggregate. This article discusses the use of budding yeast as a robust proxy to study the intersection between proteostasis and neurodegenerative disease.

Quorum protection, growth and survival

January 23, 2015

For the growth of a cell culture, one inoculates not with one cell but with a quorum of cells. This most often a requirement, not just a convenience, and most of us take this for granted without question. Here this observation is re-examined to understand why a quorum may be required to grow cells. The importance of quorums may be widespread in the aspects of microbiology they affect. It is very likely that quorums are connected with and have a large impact on the determination of Minimal Inhibitory Concentrations. It is also possible that low cell density may adversely affect cell survival, however, this is an area where even less is known. The need for a quorum might affect other aspects of microbial cell culture, cell isolation and cell preservation. Effects also extend to mammalian cell culture. Here I seek to review studies that have been documented and speculate on how the information might be utilized in the future.